The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes

Crampton, Jason; Davies, Kay E.; Knapp, Teresa

doi:10.1093/nar/9.15.3821

Cited by 43 publications

(14 citation statements)

References 28 publications

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“…M33336). The most distant splice junction occurs in the 5Ј UTR (10,15,16). These elements may be found in all mRNA regions, with the highest probability of occurrence in the 3Ј UTR and the lowest in coding sequences.…”

Section: Resultsmentioning

confidence: 99%

Evolutionary parameters of the transcribed mammalian genome: An analysis of 2,820 orthologous rodent and human sequences

Makałowski

Boguski

1998

Proc. Natl. Acad. Sci. U.S.A.

415

231

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We have rigorously defined 2,820 orthologous mRNA and protein sequence pairs from rats, mice, and humans. Evolutionary rate analyses indicate that mammalian genes are evolving 17-30% more slowly than previous textbook values. Data are presented on the average properties of mRNA and protein sequences, on variations in sequence conservation in coding and noncoding regions, and on the absolute and relative frequencies of repetitive elements and splice sites in untranslated regions of mRNAs. Our data set contains 1,880 unique human͞rodent sequence pairs that represent about 2-4% of all mammalian genes. Of the 1,880 human orthologs, 70% are present on a new gene map of the human genome, thus providing a valuable resource for cross-referencing human and rodent genomes. In addition to comparative mapping, these results have practical applications in the interpretation of noncoding sequence conservation between syntenic regions of human and mouse genomic sequence, and in the design and calibration of gene expression arrays.

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Section: Resultsmentioning

confidence: 99%

Evolutionary parameters of the transcribed mammalian genome: An analysis of 2,820 orthologous rodent and human sequences

Makałowski

Boguski

1998

Proc. Natl. Acad. Sci. U.S.A.

415

231

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“…Only 1% of the selected cDNAs detectably hybridized to radiolabeled total human genomic DNA (data not shown). In contrast, between 3% and 5% of the starting cDNA clones contain repetitive elements (23).…”

Section: Resultsmentioning

confidence: 99%

Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.

Lovett¹,

Kere²,

Hinton³

1991

Proc. Natl. Acad. Sci. U.S.A.

250

127

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“…However, a pre-screen for these was not conducted prior to sequencing. The 10.3% frequency of Alu repeat-containing clones is not significantly different than their frequency in conventional cDNA libraries (Crampton et al 1981). We have chosen conservatively aVarious classes of DNA sequences identified by searches using the BLAST-X, BLAST-N, and FASTA programs for all sequenced DNAs.…”

Section: Libraries By Sequence Analysismentioning

confidence: 99%

Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.

et al. 1995

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To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome S-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome S genes and 138 are from new chromosome S genes (a frequency of 79.5%}. Sequence complexity estimates indicate that each library contains -20% of the -SO00 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome S and describes an important new tool for disease gene isolation.

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The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes

Abstract: A library of cloned cDNAs representative of lymphocyte total

Cited by 43 publications

References 28 publications

Evolutionary parameters of the transcribed mammalian genome: An analysis of 2,820 orthologous rodent and human sequences

Evolutionary parameters of the transcribed mammalian genome: An analysis of 2,820 orthologous rodent and human sequences

Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.

Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.

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