The Occurrence of Intranuclear Crystals in Living HeLa Cells Infected with Adenoviruses

Leuchtenberger, Cecilie; Boyer, Georgiana S.

doi:10.1083/jcb.3.2.323

Cited by 19 publications

(12 citation statements)

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“…The results of the comparative studies revealed that the first specific fluores-4 The appearance of such "crystals" under interference contrast microscopy has been described in an earlier report (16).…”

Section: Fluorescent Antibody Investigationsmentioning

confidence: 88%

“…According to the latter investigators, the barshaped crystals were not composed of viral particles but of very fine particulate matter, possibly representing protein molecules. Cytochemical evidence has indicated that the bar-shaped crystals do not contain detectable amounts of deoxyribonucleic acid (16,17).…”

Section: Discussionmentioning

confidence: 99%

“…One strain evoked a sequence of nuclear alterations almost identical with that resulting from infection with adenovirus types 1 and 2 (2). The prototype 5 strain differed in that it produced an additional feature in the majority of infected nuclei: prominent bar-shaped eosinophilic and Feulgen-negative crystals, which have been observed also in fixed and in fresh HeLa cells by interference contrast microscopy (16) and in thin sectioned HeLa cells by electronmicroscopy (17). The third strain occupied an intermediate position in that it caused the formation of bar-shaped crystals in some, but not in the majority, of cells.…”

Section: Adenovirus Type 5 Light Microscopic Studies Of Fixed and Stamentioning

confidence: 99%

“…The crystals characteristic of infection with adenovirus types 3, 4, and 7, which were quite vulnerable to distortion during fixation (2), could be observed most satisfactorily in fresh unfixed preparations (13,16). Because electronmicroscopic evidence indicates that these crystals are composed of viral particles (14, 19, and 20), their formation is of particular interest and will be discussed in detail.…”

Section: Phase Contrast Microscopic Studies Of Unfixed Hela and Amniomentioning

confidence: 99%

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SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

Boyer¹,

Denny²,

Ginsberg³

1959

The Journal of Experimental Medicine

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Investigations of the biologic characteristics of adenoviruses types 1 to 4 have suggested that natural subdivisions exist within the adenovirus group (1-4). Types 1 and 2 have been found to comprise one subgroup, in that they have similar growth cycles, produce almost identical cytological changes in HeLa cells, and react quantitatively in the same manner with their typespecific neutralizing antibodies. Types 3 and 4, which constitute a second subgroup, resemble each other in the biologic aspects mentioned, yet differ from types 1 and 2. In order to determine whether types 5 and 7 adenoviruses fall into the same subgroups, the cytologic changes they evoked in HeLa cells were studied by light and phase-contrast microscopy. The role of the observed changes in viral development was then investigated by means of the fluoresceinlabelled antibody technique for the localization of intracellular antigen (5). Experiments were also undertaken to explore: (a) the possibility that the alterations produced by the prototype adenovirus strains might not be representative and that other strains of the same virus types might induce different cellular changes, and (b) the possibility that the alterations observed were peculiar to cells of the HeLa strain, which are cells in continuous culture derived from malignant tissue. Additional strains of adenoviruses types 5 and 7 were therefore tested, and the sequence of cytologic changes were followed in human amniotic cells in primary culture.The results of phase and light microscopic investigations which are here described indicate that type 7 resembles types 3 and 4, and that type 5 con-*

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Section: Fluorescent Antibody Investigationsmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Adenovirus Type 5 Light Microscopic Studies Of Fixed and Stamentioning

confidence: 99%

Section: Phase Contrast Microscopic Studies Of Unfixed Hela and Amniomentioning

confidence: 99%

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SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

Boyer¹,

Denny²,

Ginsberg³

1959

The Journal of Experimental Medicine

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“…Protein crystals with similar periodicity and fiber diameter have previously been described with certain strains of human adenovirus type 5. 4,18,19 These crystalline arrays seen with human adenovirus type 5 have been characterized as Feulgen-negative protein crystals devoid of recognizable virus and viral antigen. Paracrystals were also described in a case report of adenovirus enteritis in two calves.…”

Section: Discussionmentioning

confidence: 99%

Lesions and Transmission of Experimental Adenovirus Hemorrhagic Disease in Black-tailed Deer Fawns

et al. 1999

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Abstract. Adenovirus infection was the cause of an epizootic of hemorrhagic disease that is believed to have killed thousands of mule deer (Odocoileus hemionus) in California during the latter half of 1993. A systemic vasculitis with pulmonary edema and hemorrhagic enteropathy or a localized vasculitis associated with necrotizing stomatitis/pharyngitis/glossitis or osteomyelitis of the jaw were common necropsy findings in animals that died during this epizootic. To study transmission of adenovirus infection in deer and susceptibility of black-tailed deer (Odocoileus hemionus columbianus) fawns to adenovirus infection, six 3-6-month-old black-tailed fawns were divided into two treatment groups. One group was inoculated intravenously and the other group was inoculated through the mucous membranes of the eyes, nose and mouth with purified adenovirus. Each treatment group also included two additional fawns (four total) that were not inoculated but were exposed to inoculated animals (contact animals). One fawn served as a negative control. Between 4 and 16 days postinoculation, 8/10 fawns developed systemic or localized infection with lesions identical to lesions seen in animals with natural disease that died during the epizootic. Transmission was by direct contact, and the route of inoculation did not affect the incubation period or the distribution of the virus (systemic or the localized infection). Immunohistochemical analysis using polyclonal antiserum against bovine adenovirus type 5 demonstrated staining in endothelial cells of vessels in numerous tissues in animals with systemic infection and endothelial staining only in vessels subtending necrotic foci in the upper alimentary tract in animals with the localized form of the disease. All inoculated or exposed animals had staining in the tonsillar epithelium. Transmission electron microscopic examination of lung and ileum from two fawns with pulmonary edema and hemorrhagic enteropathy demonstrated endothelial necrosis and adenovirus virions in endothelial cell nuclei. Adenovirus was reisolated in black-tailed deer pulmonary artery endothelial cells using lung homogenate of the first fawn that developed systemic adenovirus infection. Serum virus neutralization test results suggest that this deer adenovirus is a new serotype.Key words: Adenovirus; deer; experimental infection; hemorrhagic disease; immunohistochemistry; Odocoileus hemionus columbianus; pathology; stomatitis; transmission.We previously described an epizootic of hemorrhagic disease in California black-tailed deer (Odocoileus hemionus columbianus) that was associated with infection by a newly recognized adenovirus. 28 Two forms of the disease, a systemic and a localized form, were described. Necropsy findings in deer that died of systemic adenovirus infection were similar to lesions described in animals infected with bluetongue (BT) virus or epizootic hemorrhagic disease (EHD) virus, 10,11,14,15 and included pulmonary edema and less often hemorrhagic enteropathy. Panvasculitis with endothelial intranu...

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Adenoviren

Haagen¹

1964

Viruskrankheiten Des Menschen

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The Occurrence of Intranuclear Crystals in Living HeLa Cells Infected with Adenoviruses

Cited by 19 publications

References 2 publications

SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

Lesions and Transmission of Experimental Adenovirus Hemorrhagic Disease in Black-tailed Deer Fawns

Adenoviren

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