THE OCCURRENCE OF SOME ACID PHOSPHATASES AND DEHYDROGENASES IN THE VESICULAR‐ARBUSCULAR MYCORRHIZAL FUNGUS <i>GLOMUSMOSSEAE</i>

Macdonald, R. M.; Lewis, Michael

doi:10.1111/j.1469-8137.1978.tb02273.x

Cited by 128 publications

(53 citation statements)

References 15 publications

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“…The acid phosphatase activity associated with organelles in the immature tips of arbuscule branches could be involved in the processes of elongation and growth of the hyphae, that is in the development of the infection within the host cells. In light microscope studies, a similar intense acid phosphatase activity has been localized in the actively growing germ tubes developing from spores of G. mosseae (Macdonald and Lewis, 1978).…”

Section: Discussionmentioning

confidence: 85%

ENZYMATIC STUDIES ON THE METABOLISM OF VESICULAR‐ARBUSCULAR MYCORRHIZA. III. ULTRASTRUCTURAL LOCALIZATION OF ACID AND ALKALINE PHOSPHATASE IN ONION ROOTS INFECTED BY GLOMUS MOSSEAE (NICOL. & GERD.)

Gianinazzi

Gianinazzi‐Pearson

Dexheimer³

1979

New Phytologist

113

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SUMMARYThe ultrastructural distribution of acid and alkaline phosphatase in 6-week-old onion roots infected by Glomus mosseae has been investigated cytochennically. Significant acid phosphatase activity was only observed in the little vacuolated, immature terminal arbuscule branches of the mycorrhizal fungus whilst strong alkaline a-naphthyl phosphatase and /?-glycerophosphatase activities were localized within the vacuoles of the mature arbuscular and intercellular hyphae. In the host cells neither acid nor alkaline phosphatase distribution was modified with vesiculararbuscular mycorrhiza formation. These results are discussed in relation to previously reported mycorrhiza-specific alkaline phosphatase Gianinazzi, 1976, 1978) and the metabolism of phosphorus in vesicular-arbuscular mycorrhizal systems.

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Section: Discussionmentioning

confidence: 85%

ENZYMATIC STUDIES ON THE METABOLISM OF VESICULAR‐ARBUSCULAR MYCORRHIZA. III. ULTRASTRUCTURAL LOCALIZATION OF ACID AND ALKALINE PHOSPHATASE IN ONION ROOTS INFECTED BY GLOMUS MOSSEAE (NICOL. & GERD.)

Gianinazzi

Gianinazzi‐Pearson

Dexheimer³

1979

New Phytologist

113

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“…First, rice roots colonized with R. irregularis were treated with water or 0.5 mM P solution at 14 dpp, and the roots were subjected to fungal vital staining (MacDonald and Lewis, 1978) or WGA-FITC staining at 4 or 20 h after the treatments. The number of colonized regions was similar between vital staining and WGA-FITC staining irrespective of time or treatment (Supplemental Fig.…”

Section: Branched Arbuscules Are Metabolically Activementioning

confidence: 99%

“…Fungal viability was assessed by vital staining, which detects in situ succinate dehydrogenase activity with nitroblue tetrazolium (NBT; MacDonald and Lewis, 1978). The whole root system was stained as described previously .…”

Section: Fungal Vital Stainingmentioning

confidence: 99%

Phosphate Treatment Strongly Inhibits New Arbuscule Development But Not the Maintenance of Arbuscule in Mycorrhizal Rice Roots

et al. 2016

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ORCID IDs: 0000-0002-9997-490X (Y.K.); 0000-0001-8867-5085 (C.S.).Phosphorus (P) is a crucial nutrient for plant growth, but its availability to roots is limited in soil. Arbuscular mycorrhizal (AM) symbiosis is a promising strategy for improving plant P acquisition. However, P fertilizer reduces fungal colonization (P inhibition) and compromises mycorrhizal P uptake, warranting studies on the mechanistic basis of P inhibition. In this study, early morphological changes in P inhibition were identified in rice (Oryza sativa) using fungal cell wall staining and live-cell imaging of plant membranes that were associated with arbuscule life cycles. Arbuscule density decreased, and aberrant hyphal branching was observed in roots at 5 h after P treatment. Although new arbuscule development was severely inhibited, preformed arbuscules remained intact and longevity remained constant. P inhibition was accelerated in the rice pt11-1 mutant, which lacks P uptake from arbuscule branches, suggesting that mature arbuscules are stabilized by the symbiotic P transporter under high P condition. Moreover, P treatment led to increases in the number of vesicles, in which lipid droplets accumulated and then decreased within a few days. The development of new arbuscules resumed within by 2 d. Our data established that P strongly and temporarily inhibits new arbuscule development, but not intraradical accommodation of AM fungi.

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“…To verify that the newly synthesized NADH pool is used in the respiratory chain, we compared the activity of NADH dehydrogenase in control and treated hyphae using the cytochemical approach described by MacDonald and Lewis (1978). The basic principle of this method is that for each NADH oxidized by NADPH oxidase, electrons are transferred to nitroblue tetrazolium (NBT) which, in turn, forms blue precipitates.…”

Section: Gr24 Triggers An Increase In Nadh Dehydrogenase Activitymentioning

confidence: 99%

“…NADPH dehydrogenase (EC 1.6.99.3) activity was detected in situ as previously described (MacDonald and Lewis, 1978;Peltier and Schmidt, 1991). Briefly, a stock solution of 4 mg mL 21 NBT (Promega), Tris-HCl buffer (0.2 M pH 7.4), MgCl 2 (5 mM), and H 2 O UHQ made up to 9 mL was prepared.…”

Section: Nadph Dehydrogenase Activitymentioning

confidence: 99%

GR24, a Synthetic Analog of Strigolactones, Stimulates the Mitosis and Growth of the Arbuscular Mycorrhizal FungusGigaspora roseaby Boosting Its Energy Metabolism

et al. 2008

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Arbuscular mycorrhizal (AM) fungi are obligate biotrophs that participate in a highly beneficial root symbiosis with 80% of land plants. Strigolactones are trace molecules in plant root exudates that are perceived by AM fungi at subnanomolar concentrations. Within just a few hours, they were shown to stimulate fungal mitochondria, spore germination, and branching of germinating hyphae. In this study we show that treatment of Gigaspora rosea with a strigolactone analog (GR24) causes a rapid increase in the NADH concentration, the NADH dehydrogenase activity, and the ATP content of the fungal cell. This fully and rapidly (within minutes) activated oxidative metabolism does not require new gene expression. Up-regulation of the genes involved in mitochondrial metabolism and hyphal growth, and stimulation of the fungal mitotic activity, take place several days after this initial boost to the cellular energy of the fungus. Such a rapid and powerful action of GR24 on G. rosea cells suggests that strigolactones are important plant signals involved in switching AM fungi toward full germination and a presymbiotic state.

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THE OCCURRENCE OF SOME ACID PHOSPHATASES AND DEHYDROGENASES IN THE VESICULAR‐ARBUSCULAR MYCORRHIZAL FUNGUS GLOMUSMOSSEAE

Cited by 128 publications

References 15 publications

ENZYMATIC STUDIES ON THE METABOLISM OF VESICULAR‐ARBUSCULAR MYCORRHIZA. III. ULTRASTRUCTURAL LOCALIZATION OF ACID AND ALKALINE PHOSPHATASE IN ONION ROOTS INFECTED BY GLOMUS MOSSEAE (NICOL. & GERD.)

ENZYMATIC STUDIES ON THE METABOLISM OF VESICULAR‐ARBUSCULAR MYCORRHIZA. III. ULTRASTRUCTURAL LOCALIZATION OF ACID AND ALKALINE PHOSPHATASE IN ONION ROOTS INFECTED BY GLOMUS MOSSEAE (NICOL. & GERD.)

Phosphate Treatment Strongly Inhibits New Arbuscule Development But Not the Maintenance of Arbuscule in Mycorrhizal Rice Roots

GR24, a Synthetic Analog of Strigolactones, Stimulates the Mitosis and Growth of the Arbuscular Mycorrhizal FungusGigaspora roseaby Boosting Its Energy Metabolism

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