The occurrence of virus, interferon, and circulating antibodies in mice after experimental infection with Junin virus

Boxaca, M; Guerrero, L. B. De; Savy, Virginia

doi:10.1007/bf01242631

Cited by 10 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, previous work reported in the literature [Boxaca et al, 1973b;Barrios et al, 19851 has failed to describe an adult mouse naturally susceptible to JV infection, since mortality hardly reaches lo%, and no clinical signs or CNS alterations can be found. After assaying two mouse strains (BALB/cJ and C57BL) and confirming published findings, we were able to demonstrate adult C3H/ HeJ mouse susceptibility, which suggests the existence of further susceptible strains.…”

Section: Discussionmentioning

confidence: 87%

“…Adults, however, prove resistant and fail to present neurological signs or central nervous system (CNS) tissue damage [Boxaca et al, 1973b;Giovanniello et al, 1983;Barrios et al, 19851. In contrast, lymphocytic choriomen-ingitis (LCM) virus, the prototype of the family, induces persistent infection in suckling mice without mortality but is highly lethal for the adult mouse [Buchmeier et al, 19801. Although adult mouse resistance to JV may be abrogated by cyclophosphamide treatment [Barrios et al, 19851, the lack of naturally susceptible adults has hampered research on AHF pathogenesis, given the difficulty of working with newborn animals.…”

Section: Introductionmentioning

confidence: 98%

“…Classically, infection of the suckling mouse with Junin virus (JV), etiological agent of Argentine hemorrhagic fever (AHF) and a member of the Arenaviridae family, induces lethal meningoencephalitis compatible with a delayed-type hypersensitivity (DTH) reaction. Adults, however, prove resistant and fail to present neurological signs or central nervous system (CNS) tissue damage [Boxaca et al, 1973b;Giovanniello et al, 1983;Barrios et al, 19851. In contrast, lymphocytic choriomen-ingitis (LCM) virus, the prototype of the family, induces persistent infection in suckling mice without mortality but is highly lethal for the adult mouse [Buchmeier et al, 19801. Although adult mouse resistance to JV may be abrogated by cyclophosphamide treatment [Barrios et al, 19851, the lack of naturally susceptible adults has hampered research on AHF pathogenesis, given the difficulty of working with newborn animals. Up to now, most workers have employed BALB/c (inbred) and Rockland (outbred) strains.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Susceptible adult murine model for Junin virus

Campetella

Galassi

Sanjuán

et al. 1988

Journal of Medical Virology

View full text Add to dashboard Cite

The adult mouse model had been considered resistant to Junin virus (JV) infection. However, we found that C3H/HeJ murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype XJ JV strain, showing neurological signs and 80-90% mortality within 13 days. Neutralizing antibodies (Nt Ab) were absent, but low immunofluorescent Ab levels (1:5) were detected as from day +7. The virus could only be rescued by coculture of brain samples with Vero cells. Histopathological findings were consistent with the suckling mouse model and with a delayed-type hypersensitivity reaction. XJ inoculation by extraneural routes failed to cause disease, however, it induced Nt Abs. Ic infection with XJCL3 strain of JV attenuated for man and guinea pig, but not for mouse, induced high Nt Ab levels but not mortality. In either case, mice resisted ic XJ challenge. Thus, C3H/HeJ is the first adult mouse model susceptible to JV.

show abstract

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Susceptible adult murine model for Junin virus

Campetella

Galassi

Sanjuán

et al. 1988

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…3 treated animals were sacri ficed at each time point, together with 3 in fected, nontreated controls. Both assays were carried out on Vero cell layers [8].…”

mentioning

confidence: 99%

Protection Conferred against Junin Virus Infection in Rats

Blejer¹,

Galassi²,

Saavedra³

et al. 1984

Intervirology

View full text Add to dashboard Cite

2-day-old Wistar rats intracerebrally infected with the XJC13 strain of Junin virus exhibited a 5% survival rate which rose to 71 % after immune serum treatment. Brain viral titers were relatively unaffected by this treatment. Histologic studies showed necrosis in the cerebellum and brain cortex with mononuclear cell infiltration in both treated and nontreated groups. Beginning on day 16 postinfection, however, intracerebral perivascular gliosis foci and mild meningeal congestion were minimal in the treated animals. These findings imply that passively transferred humoral immunity leads to prompt recovery in this experimental model.

show abstract

Interferon response in the guinea pig infected with Junín virus

Dejean

Ayerra

Teyssié

1987

Journal of Medical Virology

View full text Add to dashboard Cite

The "in vivo" interferon (IFN) induction capacity of two Junín virus strains--the attenuated XJCl3 and the intermediate virulent MC2--was studied in the guinea pig experimental model. Three different doses of XJCl3 strain--2,000, 10,000, and 50,000 TCID50--and a single dose of 10,000 TCID50 of MC2 were assayed. Animals were bled from day 0 to day 14 postinjection (pi) XJCl3 groups showed a constant serum IFN response. MC2 infection showed that 16% of the animals failed to develop interferonemia. The IFN activity was alpha type in most cases. The IFN serum levels induced by the MC2 strain were always lower than those attained after XJCl3 infection. The response to the positive control assayed, Newcastle disease virus, was higher and earlier than that obtained for Junín virus strains. The highest IFN individual value, which induced 160 guinea pig IFN U/ml, was detected at day 2 following XJCl3 infection, and corresponded to the highest XJCl3 dose assayed. Average values ranged from 23 to 65 guinea pig IFN U/ml, for XJCl3 groups and 15 guinea pig IFN U/ml for the MC2 group, measured at the day of maximal response. IFN presence was studied in homogenates from brain, spleen, and lymph nodes; it was detected in organs from guinea pigs infected with XJCl3 but not in organs from MC2 infected animals. IFN levels in sera or in organs failed to correlate with the histological findings. Demonstration of viral antigens in organs of infected animals, and seroconversion of Junín virus (JV) confirmed the evolution of the disease. A significant weight loss was observed just after serum IFN disappearance.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

The occurrence of virus, interferon, and circulating antibodies in mice after experimental infection with Junin virus

Cited by 10 publications

References 9 publications

Susceptible adult murine model for Junin virus

Susceptible adult murine model for Junin virus

Protection Conferred against Junin Virus Infection in Rats

Interferon response in the guinea pig infected with Junín virus

Contact Info

Product

Resources

About