The occurrence, spatial and temporal distribution, and environmental routes of tetracycline resistance and integrase genes in Crassostrea virginica beds

Barkovskii, Andrei L.; Green, Christen; Hurley, Dorset

doi:10.1016/j.marpolbul.2010.08.016

Cited by 22 publications

(21 citation statements)

References 38 publications

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“…These data suggest the seepage and spills from the lagoons as main routes of TRG from farms to the environment. In fact, most of the TRG associated to gullies, creeks, and ponds in farms' vicinities were also detected in the Altamaha River estuary, where the watershed farms were located (Barkovskii et al 2010).…”

Section: Impacts Of Farms' Operational Practices On Frequency Of Envimentioning

confidence: 99%

Persistence and Profiles of Tetracycline Resistance Genes in Swine Farms and Impact of Operational Practices on Their Occurrence in Farms’ Vicinities

Barkovskii

Bridges²

2011

Water Air Soil Pollut

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Animal farms are recognized as a major contributor to environmental pollution with antibiotic resistance genes, the persistence at the source and the discharge routes to the environment of which need better understanding. The presence of 16 tetracycline resistance genes (TRG) was assessed in feed, feces, manure lagoons, and the vicinities of three Georgia swine farms varied on antibiotic usage and other operational characteristics. In these farms, TRG profiles of feces and lagoons were composed of the same "persistent" and "transient" genes, with the former consistently observed in farms' proximities and the latter quickly attenuated in the lagoons. Fourteen TRG incidents, 13 of which occurred in gullies, ponds, and creeks, were detected around farms. Their frequency correlated to the cumulative antibiotic usage (R 2 =0.99), number of lagoons (R 2 = 0.73), and precipitation (R 2 =0.83), but not to the size of herd or the amount of manure used for fertilization. Gene-specific amplicons of TRG from the farms' proximities were similar to those of "persistent" TRG from corresponding farms. Our data suggested the independency of TRG profiles of antibiotic usage, variability in TRG persistence in manure lagoons, correlations between operational practices and TRG incidence in farms' vicinities, and spills and seepage from lagoons as TRG environmental routes.

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Section: Impacts Of Farms' Operational Practices On Frequency Of Envimentioning

confidence: 99%

Persistence and Profiles of Tetracycline Resistance Genes in Swine Farms and Impact of Operational Practices on Their Occurrence in Farms’ Vicinities

Barkovskii

Bridges²

2011

Water Air Soil Pollut

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“…Indeed, bacteria harboring MIs have been recovered from a variety of aquatic living organisms, such as in prawns, with an Enterobacter cloacae harboring a class 1 MI (Gillings et al, 2009b); or in Corbicula with a class 1 MIs relative abundance of 4% (Wright et al, 2008); and in oysters where the uncommon class 3 MIs prevailed (Barkovskii et al, 2010). Transfers of MIs between animals and human occur and have been well reviewed by Stokes and Gillings (2011).…”

Section: Role Of the Food Chainmentioning

confidence: 99%

Integron Involvement in Environmental Spread of Antibiotic Resistance

Stalder¹,

Barraud²,

Casellas³

et al. 2012

Front. Microbio.

321

212

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The spread of antibiotic-resistant bacteria is a growing problem and a public health issue. In recent decades, various genetic mechanisms involved in the spread of resistance genes among bacteria have been identified. Integrons – genetic elements that acquire, exchange, and express genes embedded within gene cassettes (GC) – are one of these mechanisms. Integrons are widely distributed, especially in Gram-negative bacteria; they are carried by mobile genetic elements, plasmids, and transposons, which promote their spread within bacterial communities. Initially studied mainly in the clinical setting for their involvement in antibiotic resistance, their role in the environment is now an increasing focus of attention. The aim of this review is to provide an in-depth analysis of recent studies of antibiotic-resistance integrons in the environment, highlighting their potential involvement in antibiotic-resistance outside the clinical context. We will focus particularly on the impact of human activities (agriculture, industries, wastewater treatment, etc.).

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“…Bacteria, including those that confer AMR, can accumulate in bivalve molluscs through their process of filter-feeding and concentrating microorganisms (Newell and Langdon, 1996;Daramola et al, 2009;Barkovskii et al, 2010). Because some bivalves are consumed raw, it is possible that they inadvertently lead to the acquisition of AMR bacteria (Han et al, 2007;Woodring et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Both species are useful sentinels for bacterial contamination (Daramola et al, 2009;Barkovskii et al, 2010). Bivalves were stored at 4°C, and live samples were processed within 7 days of collection.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Antimicrobial Resistance of Escherichia coli in Oysters and Mussels from Atlantic Canada

Rees

Davidson

Fairbrother

et al. 2015

Foodborne Pathogens and Disease

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Acquiring antimicrobial-resistant (AMR) bacteria through consuming contaminated animal food products is an emerging public health concern, though the sources of contamination are not always clear. This study characterized the occurrence of AMR in Escherichia coli from bivalve molluscs and assessed for the possible sources in the Hillsborough river complex of Prince Edward Island, Canada in areas overlapping with an oyster fishery. Multivariable statistical analysis indicated that the probability of detecting E. coli increased as the estimated dosage of animal effluent contamination decreased. Isolates with AMR were only found from sampling sites closest to untreated human effluent sources. Twenty-seven percent (n=6 of 22) of the isolates were pathogenic, with virulence factors consistent with extraintestinal E. coli of human origin. Though there is more evidence of contamination arising from human effluent, more research is needed to identify driving sources.

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The occurrence, spatial and temporal distribution, and environmental routes of tetracycline resistance and integrase genes in Crassostrea virginica beds

Cited by 22 publications

References 38 publications

Persistence and Profiles of Tetracycline Resistance Genes in Swine Farms and Impact of Operational Practices on Their Occurrence in Farms’ Vicinities

Persistence and Profiles of Tetracycline Resistance Genes in Swine Farms and Impact of Operational Practices on Their Occurrence in Farms’ Vicinities

Integron Involvement in Environmental Spread of Antibiotic Resistance

Occurrence and Antimicrobial Resistance of Escherichia coli in Oysters and Mussels from Atlantic Canada

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