Abstract. Olive mill waste is low-quality feed and rarely used in ruminant
nutrition because of its high lignocellulose content, the existence of
anti-nutritional factors such as total polyphenol and condensed tannin, and
low protein contents. This in vitro research was conducted to valorize this waste (crude olive cake, extracted olive cake, and olive leaves) using an exogenous
fibrolytic enzyme produced from Trichoderma longibrachiatum in ruminal nutrition. The enzymatic
activity of this additive was 1161 units of
endoglucanase per millilitre, 113 units of
exoglucanase per millilitre, and 2267 units of xylanases per millilitre. This treatment was applied
by spraying substrates with four doses: 0 (control), 1 (low), 2 (medium),
and 4µL g−1 µL g−1 (high) of dry matter olive mill waste in an air-conditioned
room at 26 ∘C for 12 h before in vitro incubation. For the crude olive cake, this additive at high doses increased degradation
of 14 % of cellulose and 8 % of hemicellulose compared with the control at
12 h before the in vitro incubation. Consequently, it increased dry matter
solubility and reduced sugars at this period compared to the control. Upon
ruminal incubation, the high dose of exogenous fibrolytic enzyme increased
the gas production from the immediately soluble fraction and insoluble fraction,
the rate of gas production for the insoluble fraction, the dry matter
degradability by 26 %, the organic matter degradability by 24 %, the
metabolizable energy value by 28 %, and the microbial crude protein
production by 24 % compared with the control. For olive leaves, an exogenous
fibrolytic enzyme at medium dosage can also hydrolyse the hemicellulose
compound, release fewer sugars, and increase dry matter solubility
compared with the control at 12 h before the in vitro incubation. Upon in vitro incubation, the medium dose increased the gas production from
immediately soluble and insoluble fractions, the rate of gas production for
the insoluble fraction, the dry matter degradability by 13 %, the organic
matter degradability by 11 %, the metabolizable energy value by 12 %,
and the microbial crude protein production by 12 % compared with the
control. However, the highest dose altered the gas production from insoluble
fractions and decreased microbial crude protein production by 6 % compared
with the control. Under the same conditions, an exogenous fibrolytic
enzyme applied to extracted olive cake did not produce any effect in the chemical
composition and nutritional value. These results showed clearly that
effectiveness of exogenous fibrolytic enzyme varied with incubated waste.
Increasing the nutritional value of crude olive cake and olive leaves using an
exogenous fibrolytic enzyme can encourage breeders to use this waste as
feed at a low cost in animal nutrition. This valorization of waste is a good
solution to reduce pollution of soils and groundwater caused by throwing out
this polluted waste into the environment.