The oncogenic potential of three different 7,12-dimethylbenz(a)anthracene transformed C3H/10T cell clones at various passages and the importance of the mode of immunosuppression

Saxholm, Harald J.K.

doi:10.1016/0014-2964(79)90087-2

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…It has been reported that the different transformants used in this study have different capacities for forming tumours in immunosuppressed syngeneic mice (see Saxholm, 1979 serum. All cell lines were subcultured 3 times a week.…”

Section: Methodsmentioning

confidence: 87%

Photodynamic effects of haematoporphyrin derivative on synchronized and asynchronous cells of different origin

Christensen¹,

Feren²,

Moan³

et al. 1981

Br J Cancer

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Summary.-Phototherapy in the presence of haematoporphyrin derivative has been shown to have a preferential effect on malignant tumours when compared to normal tissue. This communication presents a comparison of the sensitivity to photochemotherapy in vitro of different cell lines.Asynchronous populations of cells were exposed to light in the presence of haematoporphyrin derivative, and found to be inactivated with a comparable efficiency. The lines were of human, Chinese-hamster or mouse origin and had different abilities to form tumours after heterotransplantation into nude mice or transplantation into syngeneic, immunosuppressed mice. Synchronized cells from 4 of the lines showed a similar variation in sensitivity to light throughout the cell cycle. Cells near the middle of interphase showed the highest sensitivity, whilst cells in early G1 were found to be least sensitive towards treatment with haematoporphyrin derivative and light.

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Section: Methodsmentioning

confidence: 87%

Photodynamic effects of haematoporphyrin derivative on synchronized and asynchronous cells of different origin

Christensen¹,

Feren²,

Moan³

et al. 1981

Br J Cancer

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“…The biological relevance of the relationship between the acquisition of tumorigenicity and the altered in vivo cellfibrin interactions would be clarified further by the analysis of a cell system in which tumorigenicity could be modulated. This is the case with the murine C3WlOT1/2 cell line that is widely used in the study of chemical- (Mondal et al, 1976;Saxholm, 1979; Saxholm and Digernes, 1980) and radiation-induced (Terzaghi and Little, 1976;Brouty-Boy6 et al, 1979a;Kennedy et al, 1981) carcinogenesis. C3W10T1/2 is an established murine, density-inhibited, non-tumorigenic cell line (Reznikoff et al, 1973;Brouty-Boy6 et al, 1979a) which after X-irradiation grows in agarose and causes tumors (Brouty-Boy6 et al, 1979a).…”

mentioning

confidence: 99%

Relationship between fibrin clot retraction and tumorigenesis in C3H/10T1/2 cells

Azzarone

Brouty‐Boyé²,

Macieira‐Coelho

1981

Intl Journal of Cancer

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Fibrin clot retraction (FCR) was studied in C3H/10T1/2 murine fibroblasts. These cells were able to induce FCR with high efficiency during resting phase as well as during active growth. C3H/10/T1/2 cells which became tumorigenic after X-irradiation lacked FCR activity. Non-tumorigenic revertant clones regained the capacity to induce FCR, while revertants which progressively back-reverted to the neoplastic state showed intermediate FCR activity. These unstable revertants, when again fully tumorigenic, completely lost FCR activity.

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“…Further, the difference in both the percentage of transformed cell lines that are tumorigenic and the percentage of tumorigenic transformants that are susceptible to immune surveillance when transformants are induced by BPDE as compared to 5-AZC indicates that the transforming agent can affect both the correlation between the expression of transformation and tumorigenicity, and the interaction between the immune system and tumorigenic transformants.The transformation of contact-inhibited cells to cells that express non-contact-inhibition is widely used to assess the transforming potential of carcinogens and to study the process of carcinogenesis. Although the absence of contact-inhibition in vifro is often associated with the expression of tumorigenicity in viva, there are enough data to indicate that this correlation is not absolute (Reznikoff er al., 1973; Terrzaghi and Little, 1976;Saxholm, 1979; Friedrich et ul., 1985;Collins et al, 1982Collins et al, ,1986. There are two possible reasons why noncontact-inhibited cells fail to grow as tumors.…”

mentioning

confidence: 99%

“…The transformation of contact-inhibited cells to cells that express non-contact-inhibition is widely used to assess the transforming potential of carcinogens and to study the process of carcinogenesis. Although the absence of contact-inhibition in vifro is often associated with the expression of tumorigenicity in viva, there are enough data to indicate that this correlation is not absolute (Reznikoff er al., 1973; Terrzaghi and Little, 1976;Saxholm, 1979; Friedrich et ul., 1985;Collins et al, 1982Collins et al, ,1986. There are two possible reasons why noncontact-inhibited cells fail to grow as tumors.…”

mentioning

confidence: 99%

Anti‐tumor surveillance of non‐contact‐inhibited transformed cell lines

1988

Intl Journal of Cancer

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In order to determine if the correlated expression of transformation and tumorigenicity is affected by the agents used to induce transformants or by the immune status of the host used to test the tumorigenicity of transformants, we derived a series of cloned cell lines from foci of transformed cells induced by treatment of the contact-inhibited mouse cell line B/C-N7.ICI with the DNA demethylating agent 5-azacytidine (5-AZC) or the DNA demethylating and mutating agent benzo(a)pyrene dihydrodiol epoxide (BPDE). The transformed cell lines were injected into syngeneic nude and normal mice to determine their tumorigenicity. The results of this analysis showed that 93% of the transformants induced by 5-AZC treatment grew as tumors when injected into nude mice. Of those lines capable of growing as tumors in nude mice, 86% were also tumorigenic when injected into normal mice. In contrast, only 64% of BPDE-induced transformants grew as tumors in nude mice, and of those, only 44% were also tumorigenic in normal mice. The existence of non-contact-inhibited transformants that are tumorigenic only in nude mice indicates that host anti-tumor immune surveillance mechanisms are operative in normal mice. Further, the difference in both the percentage of transformed cell lines that are tumorigenic and the percentage of tumorigenic transformants that are susceptible to immune surveillance when transformants are induced by BPDE as compared to 5-AZC indicates that the transforming agent can affect both the correlation between the expression of transformation and tumorigenicity, and the interaction between the immune system and tumorigenic transformants.

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The oncogenic potential of three different 7,12-dimethylbenz(a)anthracene transformed C3H/10T cell clones at various passages and the importance of the mode of immunosuppression

Cited by 11 publications

References 19 publications

Photodynamic effects of haematoporphyrin derivative on synchronized and asynchronous cells of different origin

Photodynamic effects of haematoporphyrin derivative on synchronized and asynchronous cells of different origin

Relationship between fibrin clot retraction and tumorigenesis in C3H/10T1/2 cells

Anti‐tumor surveillance of non‐contact‐inhibited transformed cell lines

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