The Open Field Test

Võikar, Vootele; sC, Stanford

doi:10.31234/osf.io/8m52y

Cited by 2 publications

(3 citation statements)

References 154 publications

(166 reference statements)

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“…The test is used to measure locomotion, anxiety, explorative and stereotypical behaviors such as grooming and rearing in rodents (Walsh and Cummins, 1976;Voikar and Stanford, 2021). The behavioral apparatus consisted of four 50 cm × 50 cm arenas (with wall height of 40 cm) placed under camera for recording.…”

Section: Open Field With Objectmentioning

confidence: 99%

Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice

Nigri

Åhlgren

Wolfer

et al. 2022

Front. Behav. Neurosci.

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Behavioral phenotyping of mice has received a great deal of attention during the past three decades. However, there is still a pressing need to understand the variability caused by environmental and biological factors, human interference, and poorly standardized experimental protocols. The inconsistency of results is often attributed to the inter-individual difference between the experimenters and environmental conditions. The present work aims to dissect the combined influence of the experimenter and the environment on the detection of behavioral traits in two inbred strains most commonly used in behavioral genetics due to their contrasting phenotypes, the C57BL/6J and DBA/2J mice. To this purpose, the elevated O-maze, the open field with object, the accelerating rotarod and the Barnes maze tests were performed by two experimenters in two diverse laboratory environments. Our findings confirm the well-characterized behavioral differences between these strains in exploratory behavior, motor performance, learning and memory. Moreover, the results demonstrate how the experimenter and the environment influence the behavioral tests with a variable-dependent effect, often with mutually exclusive contributions. In this context, our study highlights how both the experimenter and the environment can have an impact on the strain effect size without altering the direction of the conclusions. Importantly, the general agreement on the results is reached by converging evidence from multiple measures addressing the same trait. In conclusion, the present work elucidates the contribution of both the experimenter and the laboratory environment in the intricate field of reproducibility in mouse behavioral phenotyping.

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Section: Open Field With Objectmentioning

confidence: 99%

Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice

Nigri

Åhlgren

Wolfer

et al. 2022

Front. Behav. Neurosci.

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“…Donepezil and MBA121 boost the effects of ACh, a neurotransmitter that is noticeably depleted in AD patients. The Open Field task (OF) is frequently used in neurobiology for screening the effect on spontaneous locomotor activity of novel drugs delivery [47]. The OF examines locomotor activity in rodents, providing straightforward quantitative measurement of variables such as distance travelled and speed.…”

Section: Donepezil Vs Mba121: Locomotor Activitymentioning

confidence: 99%

“…The OF allows to measure the characteristics of movements, both in the peripheral and central zones of a 40 × 30 × 30 cm polyvinyl chloride box. Evaluation time was 5 min, and movement analysis were performed using a software Ethovision XT© (Noldus, Wageningen, The Netherlands) following a previously described procedure [47]. Parameters such as distance walked and speed of the animals were quantitatively evaluated.…”

Section: Behavioural Experimentsmentioning

confidence: 99%

The Proof-of-Concept of MBA121, a Tacrine–Ferulic Acid Hybrid, for Alzheimer’s Disease Therapy

Rodríguez-Ruiz,

Herrero-Labrador,

Fernández-Fernández

et al. 2023

IJMS

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Great effort has been devoted to the synthesis of novel multi-target directed tacrine derivatives in the search of new treatments for Alzheimer’s disease (AD). Herein we describe the proof of concept of MBA121, a compound designed as a tacrine–ferulic acid hybrid, and its potential use in the therapy of AD. MBA121 shows good β-amyloid (Aβ) anti-aggregation properties, selective inhibition of human butyrylcholinesterase, good neuroprotection against toxic insults, such as Aβ1–40, Aβ1–42, and H2O2, and promising ADMET properties that support translational developments. A passive avoidance task in mice with experimentally induced amnesia was carried out, MBA121 being able to significantly decrease scopolamine-induced learning deficits. In addition, MBA121 reduced the Aβ plaque burden in the cerebral cortex and hippocampus in APPswe/PS1ΔE9 transgenic male mice. Our in vivo results relate its bioavailability with the therapeutic response, demonstrating that MBA121 is a promising agent to treat the cognitive decline and neurodegeneration underlying AD.

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The Open Field Test

Cited by 2 publications

References 154 publications

Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice

Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice

The Proof-of-Concept of MBA121, a Tacrine–Ferulic Acid Hybrid, for Alzheimer’s Disease Therapy

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