The Optimal Strategy of Noninvasive Limb Ischemic Preconditioning for Protecting Heart Against Ischemia-Reperfusion Injury in Rats

Gao, Jianbo; Kang, Yi; Jian-shi, Lou

doi:10.1016/j.jss.2011.10.015

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…Non-invasive blood pressure measurements used a cuff around the thigh of the left hind limb in anesthetized rats, and a pulse sensor was placed over the dorsal artery on the surface of the foot (BL-420E Biofunction Laboratory System, Taimeng Technology Co., China) to perform cardiac remote ischemic conditioning (RIC). RIC was repeated using 5 min of inflation in the left femoral artery and 5 min of deflation for four cycles once daily on days 1, 7, and 14 of the study, as previously described [9]. Skin cyanosis and lower limb temperature indicated successful ischemia, and skin flushing and an increase in limb temperature indicated successful reperfusion.…”

Section: Methodsmentioning

confidence: 99%

The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction

Pan

et al. 2019

Med Sci Monit

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BackgroundCardiac remote ischemic conditioning (RIC) is a noninvasive cardioprotective method in ischemia-reperfusion injury and acute myocardial infarction (AMI). The aims of this study were to investigate the effects of RIC in a rat model of AMI.Material/MethodsAdult male Sprague-Dawley rats included the AMI group that underwent ligation of the left anterior descending (LAD) coronary artery (n=24), the RIC group that consisted the AMI rat model treated with RIC once daily in the left hind limb until days 1, 7 and 14 (n=24), and the sham group (n=24). Myocardial infarct size was measured by routine histology with triphenyltetrazolium chloride (TTC) and Masson’s trichrome histochemical staining for myocardial necrosis and fibrosis, respectively. Serum levels of Bcl-2, Bax, caspase-3, and inducible nitric oxide synthase (iNOS) were measured by enzyme-linked immunosorbent assay (ELISA). The apoptosis index was detected using the TUNEL assay. Spectrophotometry of the myocardium was used to identify mitochondrial complexes and myocardial ATP.ResultsThe RIC group showed improved cardiac hemodynamics, reduced the size of the myocardial infarction, upregulated expression of Bcl-2, and down-regulation of the levels of Bax, caspase-3, and iNOS, and reduced cardiac myocyte apoptosis and inhibited the opening of the mitochondrial permeability transition pore (MPTP).ConclusionsIn a rat model of AMI, RIC improved the hemodynamic index, reduce the levels of apoptosis and myocardial injury, and improved mitochondrial function.

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Section: Methodsmentioning

confidence: 99%

The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction

Pan

et al. 2019

Med Sci Monit

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“…[ 12 ] Many experiments have been conducted to confirm that iPreCo improves tolerance in various animal organs and tissues. [ 13–20 ] Przyklenk et al . demonstrated protective effects of iPreCo on the myocardium by inducing iPreCo with four cycles of 5‐min ischaemia and 5‐min reperfusion on circumflex branch.…”

Section: Preconditioningmentioning

confidence: 99%

Pharmacological postconditioning: a molecular aspect in ischemic injury

Khan

Kashyap

Kaur

et al. 2020

Journal of Pharmacy and Pharmacology

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Objective Ischaemia/reperfusion (I/R) injury is defined as the damage to the tissue which is caused when blood supply returns to tissue after ischaemia. To protect the ischaemic tissue from irreversible injury, various protective agents have been studied but the benefits have not been clinically applicable due to monotargeting, low potency, late delivery or poor tolerability. Key Findings Strategies involving preconditioning or postconditioning can address the issues related to the failure of protective therapies. In principle, postconditioning (PoCo) is clinically more applicable in the conditions in which there is unannounced ischaemic event. Moreover, PoCo is an attractive beneficial strategy as it can be induced rapidly at the onset of reperfusion via series of brief I/R cycles following a major ischaemic event or it can be induced in a delayed manner. Various pharmacological postconditioning (pPoCo) mechanisms have been investigated systematically. Using different animal models, most of the studies on pPoCo have been carried out preclinically. Summary However, there is a need for the optimization of the clinical protocols to quicken pPoCo clinical translation for future studies. This review summarizes the involvement of various receptors and signalling pathways in the protective mechanisms of pPoCo.

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“…Clinically, Gao et al showed that the ideal sequence for maximizing the benefits of IPC is one day of remote IPC followed by a one-day interval before surgery. 69 Research from cardiothoracic surgery determined that IPC must last at least two minutes to confer a benefit to the patient. 70 IPC reduces the release of CK-MB following CABG and stimulates left ventricular HSP-72 protein expression.…”

Section: Heartmentioning

confidence: 99%

The Delay Phenomenon: A Compilation of Knowledge across Specialties

Hamilton

Wolfswinkel

Weathers

et al. 2014

Craniomaxillofacial Trauma & Reconstruction

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Objective The purpose of this article is to review and integrate the available literature in different fields to gain a better understanding of the basic physiology and optimize vascular delay as a reconstructive surgery technique. Methods A broad search of the literature was performed using the Medline database. Two queries were performed using "vascular delay," a search expected to yield perspectives from the field of plastic and reconstructive surgery, and "ischemic preconditioning," (IPC) which was expected to yield research on the same topic in other fields. Results The combined searches yielded a total of 1824 abstracts. The "vascular delay" query yielded 76 articles from 1984 to 2011. The "ischemic preconditioning" query yielded 6534 articles, ranging from 1980 to 2012. The abstracts were screened for those from other specialties in addition to reconstructive surgery, analyzed potential or current uses of vascular delay in practice, or provided developments in understanding the pathophysiology of vascular delay. 70 articles were identified that met inclusion criteria and were applicable to vascular delay or ischemic preconditioning. Conclusion An understanding of IPC's implementation and mechanisms in other fields has beneficial implications for the field of reconstructive surgery in the context of the delay phenomenon. Despite an incomplete model of IPC's pathways, the anti-oxidative, anti-apoptotic and anti-inflammatory benefits of IPC are well recognized. The activation of angiogenic genes through IPC could allow for complex flap design, even in poorly vascularized regions. IPC's promotion of angiogenesis and reduction of endothelial dysfunction remain most applicable to reconstructive surgery in reducing graft-related complications and flap failure.

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The Optimal Strategy of Noninvasive Limb Ischemic Preconditioning for Protecting Heart Against Ischemia-Reperfusion Injury in Rats

Cited by 5 publications

References 27 publications

The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction

The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction

Pharmacological postconditioning: a molecular aspect in ischemic injury

The Delay Phenomenon: A Compilation of Knowledge across Specialties

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