The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed

Abstract

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg−1) and experiment 2 (3 g kg−1). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious (p < 0.05), and the average egg weight was significantly increased in experiment 2 (p < 0.05).

Fermented wheat bran by xylanase-producing Bacillus cereus boosts the intestinal microflora of broiler chickens

“…In this study, replacing 5% corn with B.cereus fermented wheat bran did not affect the growth performance of broilers. Suggesting that SSF using xylanase-producing microorganisms can degrade NSP and be used in the pre-treatment of feedstuff, which has similar standpoint to previous studies (Latorre et al, 2015 ; Supriyati et al, 2015 ; Ye et al, 2017 ). Compared with other methods to degrade NSP in diets, such as adding exogenous enzymes (Cardoso et al, 2018 ), appending microbial agents (Wealleans et al, 2017 ), and low temperature freezing (Khadem et al, 2016 ), SSF not only ensures the nutrient level of diets, but optimizes the intestinal microbiota and further improves the digestion and absorption capacity of broiler chickens.…”

Bioprocess development for enhanced endoglucanase production by newly isolated bacteria, purification, characterization and in-vitro efficacy as anti-biofilm of Pseudomonas aeruginosa

“…These results are in agreement with other studies performed on cellulase production by Bacillus spp. Optimum temperature for production of cellulase by B. amyloliquefaciens DL-3 49 , Bacillus pumilus EB3 50 and Bacillus amyloliquefaciens 51 was 37 °C. Islam et al 52 reported maximum enzyme production by Bacillus sp.…”

Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans

“…The cecum of chicken has more diversity of LAB than the small intestine, and LAB isolated from poultry cecum have been reported to have higher enzyme secretion and activity than other probiotics [17,18]. In this study, an Enterococcus strain, L11, was isolated from cecum of a healthy chicken and identified as E. faecium.…”