Reverse osmosis (RO) is one of the advanced wastewater treatment processes that remove the concentrate in the form of dissolved impurities in raw waste water. The treated reject released during the RO process remain a major polluting problem. The RO reject, with high solid concentration, needs maximum purification. It is one major problem prevailing in the dye industry where RO was mainly used as a tertiary treatment to achieve zero discharge effluent. Approximately one-third of the total quantity of wastewater concentration was rejected and collected as RO reject in dye industries. This concentrated RO rejects stream seems to be more toxic than non-treated wastewater. To overcome this difficulty anaerobic baffled wall reactor (ABR) study was initiated. It was necessary to find a potential solution to treat RO reject with by-product recovery as the existing methods for treating RO reject is open dumping in the sea, discharge in sewer or swamps, as a dewatering process, disposal in water bodies. ABR study was carried out in a lab scale using RO as a feeding material. In this investigation, the anaerobic bio treatment process was carried out to reduce the Biological Oxygen Demand (BOD) concentration. ABR has the advantage of operating at even reduced flow rates to ensure contact between the RO reject feed and micro-organism. As a result of anaerobic digestion, ABR will produce a biogas as a by-product. By investigating various characteristics, it was observed that there is a consequential percentage decrease in Biological oxygen demand (55% -80%), Chemical oxygen demand (COD) (65% -80%), Chlorides (75% -90%) and Alkalinity (75% -85%).