The optimized preparation of HA/L-TiO2/D-TiO2 composite coating on porous titanium and its effect on the behavior osteoblasts

Abstract

Various surface bioactivation technology has been confirmed to improve the osteogenic ability of porous titanium (pTi) implants effectively. In this study, a three-layered composite coating, i.e. outer layer of hydroxyapatite (HA), middle layer of loose titanium dioxide (L-TiO2) and inner layer of dense TiO2 (D-TiO2), was fabricated on pTi by a combined processing procedure of pickling, alkali heat (AH), anodic oxidation (AO), electrochemical deposition (ED) and hydrothermal treatment (HT). After soaking in simulated body fluid for 48 h, the surface of the AHAOEDHT-treated pTi was completely covered by a homogeneous apatite layer. Using MC3T3-E1 pro-osteoblasts as cell model, the cell culture revealed that both the pTi without surface treatment and the AHAOEDHT sample could support the attachment, growth and proliferation of the cells. Compared to the pTi sample, the AHAOEDHT one induced higher expressions of osteogenesis-related genes in the cells, including alkaline phosphatase, Type I collagen, osteopontin, osteoclast inhibitor, osteocalcin and zinc finger structure transcription factor. As thus, besides the good corrosion resistance, the HA/L-TiO2/D-TiO2-coated pTi had good osteogenic activity, showing good potential in practical application for bone defect repair.

Surface Modification Techniques to Produce Micro/Nano-scale Topographies on Ti-Based Implant Surfaces for Improved Osseointegration

“…By changing the Acid etching or alkali-heat treatment parameters, such as immersion temperature, duration, acid or alkali concentration, and heating furnace temperature, the surface of different scales or morphologies can be formed (Zhuang et al, 2014;Kato et al, 2015;Fu et al, 2020). A common acid etching treatment is to soak the titanium-based implants in a strong acid solution for 60 min at 60-100 °C and ultrasonic vibration (Souza et al, 2019).…”

Comparative study of cell interaction and bacterial adhesion on titanium of different composition, structure and surfaces with various laser treatment

“…It has been found that materials with a combination of micro-and nanostructured surfaces with a certain roughness can show improved levels of cell attachment [13]. Methods such as sandblasting and acid etching [14,15], anodic oxidation [16], plasma spraying [17], magnetron sputtering [18], or meniscus-dragging deposition [19] are commonly used for surface modification. These methods assume contact with the material, so they have some limitations to their universal use such as limitations in substrate size or contamination with hazardous chemicals [20].…”

Aligned TiO2 Scaffolds in the Presence of a Galactopyranose Matrix by Sol-Gel Process

“…In this way, various strategies have been created to stimulate the antibacterial properties of TiO 2 scaffolds in the presence of PLGA [ 28 ]. Among other things, the osteogenic ability of Ti has been studied to assess the effects of osteoblastic activity on this material [ 29 ]. Likewise, the analysis of the surface of Ti compounds to improve the contact of biomaterials has been evaluated by means of techniques, such as plasma and anodic oxidation [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”