The optimum threonine requirement in diets of juvenile hybrid grouper (
            <i>epinephelus fuscoguttatus ♀</i>
            ×
            <i>Epinephelus lanceolatus ♂</i>
            )

Ma, Lei; Wu, Xiaoyi; Ye, Bo; Geng, Long; Zhou, Zhiyu; Xiao, Wang; Mu, Wei

doi:10.1111/anu.13227

Cited by 9 publications

(12 citation statements)

References 54 publications

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“…Weight gain in the fish body is primarily attributed to protein and fat deposition (Bureau et al, 2000), which leads to improvements in other growth parameters and better feed utilization in cultured animals, similar to the findings for Indian major carp (Abidi & Khan, 2008), blunt snout bream (Habte‐Tsion, Liua, et al, 2015), stinging catfish (Khan, 2017), hybrid catfish (Zhao et al, 2020) and largemouth bass (Rahman et al, 2021). No significant difference was observed by the supplementation of dietary threonine (Lei et al, 2021). Supplementing dietary threonine in grass carp resulted in increased growth (Dong et al, 2017), yet too much threonine in the diet was not favourable to better growth.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of dietary L‐threonine in rohu ( Labeo rohita ): Effects of stocking density on nutritional physiology, immune response, antioxidant system and disease resistance against Aeromonas hydrophila

Swamy

Hanumappa

Rathore

2022

Aquaculture Research

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A 60-day feeding trial was carried out to examine the ameliorative effects of graded levels of dietary L-threonine [T1 = 7.3 (un-supplemented), T2 = 10.6, T3 = 13.5 and T4 = 16.4 g/kg feed] on immune response, nutritional physiology and disease resistance in rohu (Labeo rohita) fingerlings against different stocking densities and Aeromonas hydrophila. A total of 504 numbers of uniform-sized rohu fingerlings (3.0 ± 0.03 g) were stocked at two stocking densities, that is, in high stocking density (30 numbers/tank) and low stocking density (12 numbers/tank) in triplicate groups

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Section: Discussionmentioning

confidence: 99%

Supplementation of dietary L‐threonine in rohu ( Labeo rohita ): Effects of stocking density on nutritional physiology, immune response, antioxidant system and disease resistance against Aeromonas hydrophila

Swamy

Hanumappa

Rathore

2022

Aquaculture Research

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“…The experiment diets ( Table 1 ) were designed to have same crude protein (CP, 464.7 g/kg), crude lipid (CL, 70 g/kg), and gross energy (340 kcal/100 g dry matter), referring to previous studies [ 26 – 28 , 30 , 31 ]. The overall dietary amino acid contents ( Table 2 ) were adjusted accordingly based on our researches on the reference amino acid profile [ 24 ] and the optimum dietary requirements for leucine [ 25 ], methionine [ 27 ], arginine [ 28 ], and threonine [ 30 ]. Alanine was added as the substitute in diets with low Phe.…”

Section: Methodsmentioning

confidence: 99%

“…The well-ground fishmeal was used during the preparation, along with careful weighing of all the dry ingredients. Dietary preparation was conducted according to the reference methods in our previous study [ 30 ]. The bound CAAs was mixed with other dry ingredients and recoated with 229.6–233.7 g corn starch and another 10 g CMC for every 1,000 g dry matter, and then mixed with lipids and water for pelletization.…”

Section: Methodsmentioning

confidence: 99%

“…Yield determination was performed using NanoDrop 2000c (Thermo, America). An optical density ratio of 1.8–2.0 was observed between 260 and 280 according to the electrophoresis bands, and luminance detection integrity of total RNA was observed at 28, 18, and 5 s. Following the manufacturer's instructions, reverse transcription reaction (Takara, Japan) and RT–qPCR were performed (Quant Studio 6 Flex, Applied Biosystems, Singapore) [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

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The Optimum Dietary Phenylalanine Requirement of Hybrid Grouper (Epinephelusfuscoguttatus ♀ × Epinepheluslanceolatus ♂) Juveniles: Effects on Growth Performance, Gut Micromorphology, and Antioxidation

Yang

Wang

et al. 2023

Aquaculture Nutrition

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The optimum phenylalanine (Phe) requirement for hybrid grouper (Epinephelusfuscoguttatus ♀ × Epinepheluslanceolatus ♂) juveniles was determined through an 8-week growth trial. A total of seven isoenergetic (340 kcal per 100 g of dry matter), isonitrogenous, and isolipidic diets were made, containing 8.2 (Phe 8.2), 9.2 (Phe 9.2), 10.1 (Phe 10.1), 11.2 (Phe 11.2), 13.3 (Phe 13.3), 15.2 (Phe 15.2), and 17.3 g/kg (Phe 17.3), respectively. Triplicate tanks of juvenile fish (about 16.7 g/fish) were fed each experimental diet twice daily until apparent satiation. The results indicated that different dietary Phe levels significantly influenced weight gain percentage (WG), feed efficiency (FE), protein efficiency ratio (PER), as well as, productive protein value (PPV). Fish fed Phe 8.2 had the lowest WG or PPV among all experimental treatments. Furthermore, the optimal dietary Phe level increased fold height, width, enterocyte, and microvillus height of fish. The Phe 10.1 group exhibited higher growth hormone (GH) expression in the pituitary compared to other groups. Expression of hepatic insulin-like growth factor-1 (IGF-1) and growth hormone receptor 1 (GHR1) displayed a similar pattern of variation to that of GH. The Phe 13.3 group had lower expression of S6 kinase 1 (S6K1) and target of rapamycin (TOR) than other groups. In addition, fish fed Phe 10.1 had lower levels of nuclear factor erythroid 2 (Nrf2) and heat shock protein 70 (HSP70) in the head kidney, and Cu/Zn-superoxide (Cu/ZnSOD) dismutases in the midgut compared to fish fed other Phe levels. Generally, optimal Phe content in the diet of hybrid grouper was estimated to be 12.7 g/kg of dry matter (27.3 g/kg of dietary protein), and at this level, the feed utilization, gut micromorphology, and immunity of fish were also elevated.

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“…Experimental fish purchased from a local hatchery were firstly acclimated and then fed with the experimental diets for 7 weeks. The feeding procedure was the same as that described in our previous study (Lei et al, 2021). After acclimatization, experimental fish (average initial body weight: 14.30 ± 0.1 g per fish) were randomly distributed into tanks at a density of 12 fish per tank, and each dietary treatment had three replicates.…”

Section: Growth Trialmentioning

confidence: 99%

Replacing poultry by‐product meal protein with soybean protein isolate in low fishmeal diets for juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Xue

et al. 2021

Aquacult Nutr

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A 7-week growth trial was conducted to assess the potential of using soybean protein isolate (SPI) to replace poultry by-product meal (PBM) protein in low fishmeal (FM) diets for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). Seven isoproteic (490 g kg −1 ) and isolipidic (90 g kg −1 ) experimental diets were formulated, including six low FM diets and a high FM diet (HFMP). The low FM diets contained 0, 74.8, 149.7, 224.5, 299.4 and 374.2 g kg −1 SPI, replacing approximately 0%, 20%, 40%, 60%, 80% and 100% of the PBM protein (designated as SPIP0, SPIP20, SPIP40, SPIP60, SPIP80 and SPIP100, respectively). Each diet was hand-fed to triplicate groups of 12 hybrid grouper juveniles (average initial body weight: 14.30 ± 0.1 g per fish). After being fed for 7 weeks, fish fed with HFMP and SPIP60 showed significantly higher (p < .01) weight gain percentage (WG%), protein efficiency ratio and protein productive value than fish fed with other experimental diets. These three parameters were elevated with the increment in PBM protein (PBMP) replacements, reaching the peak value at the 60% replacing level, and then, they were significantly reduced at the 80% or 100% replacing level. Analysis of the two-slope straight brokenline model showed that the maximum PBMP replacement level by SPI protein (SPIP) at the intercept value of WG% was estimated to be 60%. Feed conversion ratio showed an opposite tendency of variation to WG%. Fish fed with SPIP80 and SPIP100 displayed lower mRNA levels of hepatic insulin-like growth factor-1, hypothalamus neuropeptide Y and head-kidney nuclear factor erythroid 2-related factor 2 than fish fed with other experimental diets. Generally, 60% of PBM protein could be replaced with SPI protein in low FM diets for hybrid grouper.

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The optimum threonine requirement in diets of juvenile hybrid grouper ( epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ )

Cited by 9 publications

References 54 publications

Supplementation of dietary L‐threonine in rohu ( Labeo rohita ): Effects of stocking density on nutritional physiology, immune response, antioxidant system and disease resistance against Aeromonas hydrophila

Supplementation of dietary L‐threonine in rohu ( Labeo rohita ): Effects of stocking density on nutritional physiology, immune response, antioxidant system and disease resistance against Aeromonas hydrophila

The Optimum Dietary Phenylalanine Requirement of Hybrid Grouper (Epinephelusfuscoguttatus ♀ × Epinepheluslanceolatus ♂) Juveniles: Effects on Growth Performance, Gut Micromorphology, and Antioxidation

Replacing poultry by‐product meal protein with soybean protein isolate in low fishmeal diets for juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

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