The optomotor response of aging zebrafish reveals a complex relationship between visual motion characteristics and cholinergic system

Karaduman, Ayşenur; Karoglu-Eravsar, Elif Tugce; Kaya, Utku; Aydin, Alaz; Adams, Michelle M.; Kafaligonul, Hulusi

doi:10.1016/j.neurobiolaging.2020.10.018

Cited by 8 publications

(6 citation statements)

References 103 publications

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“…Deficits have been observed as well in motion-related fMRI activation of the primary visual cortex [ 62 ]. Interestingly, vERP abnormalities beginning in the retina have also been observed in 5xFAD transgenic [ 63 ] and other [ 64 ] mice in association with amyloid deposition and in zebrafish cholinergic models [ 65 ], suggesting potential translational utility.…”

Section: Discussionmentioning

confidence: 99%

Disruption of early visual processing in amyloid-positive healthy individuals and mild cognitive impairment

et al. 2023

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Background Amyloid deposition is a primary predictor of Alzheimer’s disease (AD) and related neurodegenerative disorders. Retinal changes involving the structure and function of the ganglion cell layer are increasingly documented in both established and prodromal AD. Visual event-related potentials (vERP) are sensitive to dysfunction in the magno- and parvocellular visual systems, which originate within the retinal ganglion cell layer. The present study evaluates vERP as a function of amyloid deposition in aging, and in mild cognitive impairment (MCI). Methods vERP to stimulus-onset, motion-onset, and alpha-frequency steady-state (ssVEP) stimuli were obtained from 16 amyloid-positive and 41 amyloid-negative healthy elders and 15 MCI individuals and analyzed using time–frequency approaches. Social cognition was assessed in a subset of individuals using The Awareness of Social Inference Test (TASIT). Results Neurocognitively intact but amyloid-positive participants and MCI individuals showed significant deficits in stimulus-onset (theta) and motion-onset (delta) vERP generation relative to amyloid-negative participants (all p < .01). Across healthy elders, a composite index of these measures correlated highly (r = − .52, p < .001) with amyloid standardized uptake value ratios (SUVR) and TASIT performance. A composite index composed of vERP measures significant differentiated amyloid-positive and amyloid-negative groups with an overall classification accuracy of > 70%. Discussion vERP may assist in the early detection of amyloid deposition among older individuals without observable neurocognitive impairments and in linking previously documented retinal deficits in both prodromal AD and MCI to behavioral impairments in social cognition.

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Section: Discussionmentioning

confidence: 99%

Disruption of early visual processing in amyloid-positive healthy individuals and mild cognitive impairment

et al. 2023

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“…Tail samples were used for genomic DNA extraction and allele-specific q-PCR genotyping to distinguish ache mutants from their wildtype siblings. Genotyping experiments were carried out with respect to previously described protocols in this model [ 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

Long-Term Acetylcholinesterase Depletion Alters the Levels of Key Synaptic Proteins while Maintaining Neuronal Markers in the Aging Zebrafish (Danio rerio) Brain

Karoglu-Eravsar,

Tuz-Sasik,

Karaduman

et al. 2023

Gerontology

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Introduction: Interventions targeting cholinergic neurotransmission like acetylcholinesterase (AChE) inhibition distinguish potential mechanisms to delay age-related impairments and attenuate deficits related to neurodegenerative diseases. However, the chronic effects of these interventions are not well-described. Methods: In the current study, global levels of cholinergic, cellular, synaptic, and inflammation-mediating proteins were assessed within the context of aging and chronic reduction of AChE activity. Long-term depletion of AChE activity was induced by using a mutant zebrafish line and they were compared with the wildtype group at young and old ages. Results: Results demonstrated that AChE activity was lower in both young and old mutants and this decrease coincided with a reduction in ACh content. Additionally, an overall age-related reduction in AChE activity and the AChE/ACh ratio was observed, this decline was more prominent in wildtype groups. The levels of an immature neuronal marker were upregulated in mutants, while a glial marker showed an overall reduction. Mutants had preserved levels of inhibitory and presynaptic elements with aging, whereas glutamate receptor subunit levels declined. Discussion/Conclusion: Long-term AChE activity depletion induces synaptic and cellular alterations. These data provide further insights into molecular targets and adaptive responses following the long-term reduction of AChE activity that was also targeted pharmacologically to treat neurodegenerative diseases in human subjects.

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“…Thus, we wanted to know if melatonin had a short-term impact on the general regeneration of the visual system. Acetylcholine is one of the most important neurotransmitters in the visual system [38]. The activity of acetylcholine depends on two enzymes, one for its production: choline acetyl transferase (ChAT); and another for its degradation: acetylcholine esterase (AChE).…”

Section: Exposure To Melatonin Induces Changes In the Ot Undergoing R...mentioning

confidence: 99%

REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration

Pérez-Montes,

Jiménez-Cubides,

Velasco

et al. 2023

Antioxidants

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Zebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the role of oligodendrocytes and the signals that mediate regeneration, we use the well-established optic nerve (ON) crush model. We also used sox10 fluorescent transgenic lines to label fully differentiated oligodendrocytes. To quench the effect of reactive oxygen species (ROS), we used the endogenous antioxidant melatonin. Using these tools, we measured ROS production by flow cytometry and explored the regeneration of the optic tectum (OT), the response of oligodendrocytes and their mitochondria by confocal microscopy and Western blot. ROS are produced by oligodendrocytes 3 h after injury and JNK activity is triggered. Concomitantly, there is a decrease in the number of fully differentiated oligodendrocytes in the OT and in their mitochondrial population. By 24 h, oligodendrocytes partially recover. Exposure to melatonin blocks the changes observed in these oligodendrocytes at 3 h and increases their number and their mitochondrial populations after 24 h. Melatonin also blocks JNK upregulation and induces aberrant neuronal differentiation in the OT. In conclusion, a proper balance of ROS is necessary during visual system regeneration and exposure to melatonin has a detrimental impact.

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The optomotor response of aging zebrafish reveals a complex relationship between visual motion characteristics and cholinergic system

Cited by 8 publications

References 103 publications

Disruption of early visual processing in amyloid-positive healthy individuals and mild cognitive impairment

Disruption of early visual processing in amyloid-positive healthy individuals and mild cognitive impairment

Long-Term Acetylcholinesterase Depletion Alters the Levels of Key Synaptic Proteins while Maintaining Neuronal Markers in the Aging Zebrafish (Danio rerio) Brain

REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration

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