The organization of collagen in the corneal stroma

Meek, Keith Michael Andrew; Boote, Craig

doi:10.1016/j.exer.2003.07.003

Cited by 228 publications

(179 citation statements)

References 40 publications

Supporting

Mentioning

158

Contrasting

Unclassified

Order By: Relevance

“…In preliminary studies (data not shown) we have obtained excellent results for untreated sections as thin as 8 µm, and in 1 µm-thick sections treated with picrisirius red (to enhance collagen birefringence). Synchrotron radiation has also been used to acquire micron-scale resolution data from ocular tissues [12,28], but the super-specialized equipment needed makes it less widely available than PLM.…”

Section: Discussionmentioning

confidence: 99%

“…However, the accuracy, repeatability and robustness of the technique when applied to ocular tissues remains to be determined. Tendon and ligaments are generally similar to collagenous ocular tissues in terms of extracellular matrix composition [26], but there are differences in their structural hierarchies [9] and overall organization [4,[27][28][29]. Therefore, it cannot be taken for granted that PLM will apply to ocular tissues as it does to other tissues, and the performance of the technique must be assessed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polarization microscopy for characterizing fiber orientation of ocular tissues

Jan

Grimm

Tran

et al. 2015

Biomed. Opt. Express

150

View full text Add to dashboard Cite

Characterizing the collagen fiber orientation and organization in the eye is necessary for a complete understanding of ocular biomechanics. In this study, we assess the performance of polarized light microscopy to determine collagen fiber orientation of ocular tissues. Our results demonstrate that the method provides objective, accurate, repeatable and robust data on fiber orientation with µm-scale resolution over a broad, cmscale, field of view, unaffected by formalin fixation, without requiring tissue dehydration, labeling or staining. Together, this shows that polarized light microscopy is a powerful method for studying collagen architecture in the eye, with applications ranging from normal physiology and aging, to pathology and transplantation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polarization microscopy for characterizing fiber orientation of ocular tissues

Jan

Grimm

Tran

et al. 2015

Biomed. Opt. Express

150

View full text Add to dashboard Cite

show abstract

“…35 The collagen fibers within individual lamellae are parallel, but they have different orientations with adjacent lamellae that are stacked on top of one another. 34,36 Corneal birefringence is a combination of intrinsic and form birefringence. Intrinsic birefringence has its origin in the optical anisotropy within the collagen fibers, 35,37 which are composed predominately of type I collagen molecules that are known to exhibit positive uniaxial birefringence.…”

Section: Polarization Properties Of the Central Corneamentioning

confidence: 99%

Birefringence of the central cornea in children assessed with scanning laser polarimetry

Irsch

Shah

2012

J. Biomed. Opt

View full text Add to dashboard Cite

Abstract. Corneal birefringence is a well-known confounding factor with all polarization-sensitive technology used for retinal scanning and other intraocular assessment. It has been studied extensively in adults, but little is known regarding age-related differences. Specifically, no information is available concerning corneal birefringence in children. For applications that are geared towards children, such as retinal birefringence scanning for strabismus screening purposes, it is important to know the expected range of both corneal retardance and azimuth in pediatric populations. This study investigated central corneal birefringence in children (ages three and above), by means of scanning laser polarimetry (GDx-VCC™, Carl Zeiss Meditec, Inc.). Children's measures of corneal retardance and azimuth were compared with those obtained in adults. As with previous studies in adults, corneal birefringence was found to vary widely in children, with corneal retardance ranging from 10 to 77 nm, and azimuth (slow axis) ranging from −11°to 71°(measured nasally downward). No significant differences in central corneal birefringence were found between children and adults, nor were significant age-related differences found in general. In conclusion, establishing knowledge of the polarization properties of the central cornea in children allows better understanding, exploitation, or bypassing of these effects in new polarization-sensitive pediatric ophthalmic applications.

show abstract

“…Produced by stromal keratocytes, the collagen is structured in a highly organized manner, which is crucial for the cornea's transparency. Within the stroma 200-250 collagen lamellae house the collagen fibrils that are densely packed with a strict parallel and orthogonal orientation to allow high transparency (Meek and Boote, 2004). Descemet's membrane, like Bowman's membrane, is a cell-free layer of collagen type VIII.…”

Section: Anatomy Of the Eyementioning

confidence: 99%

Alternative methods for the replacement of eye irritation testing

Lotz¹

2016

ALTEX

View full text Add to dashboard Cite

SummaryIn the last decades significant regulatory attempts were made to replace, refine and reduce animal testing to assess the risk of consumer products for the human eye. As the original in vivo Draize eye test is criticized for limited predictivity, costs and ethical issues, several animal-free test methods have been developed to categorize substances according to the global harmonized system (GHS) for eye irritation. This review summarizes the progress of alternative test methods for the assessment of eye irritation. Based on the corneal anatomy and current knowledge of the mechanisms causing eye irritation, different ex vivo and in vitro methods will be presented and discussed with regard to possible limitations and status of regulatory acceptance. In addition to established in vitro models, this review will also highlight emerging, full thickness cornea models that might be suited to predict all GHS categories.

show abstract

The organization of collagen in the corneal stroma

Cited by 228 publications

References 40 publications

Polarization microscopy for characterizing fiber orientation of ocular tissues

Polarization microscopy for characterizing fiber orientation of ocular tissues

Birefringence of the central cornea in children assessed with scanning laser polarimetry

Alternative methods for the replacement of eye irritation testing

Contact Info

Product

Resources

About