The Origin of Elevated Levels of Circulating DNA in Blood Plasma of Premature Neonates

Tuaeva, N. O.; Zi, Abramova; Sofronov, V. V.

doi:10.1196/annals.1448.043

Cited by 24 publications

(20 citation statements)

References 12 publications

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“…Teaeva, et al reported that there only (23). However, we found that basal levels of spontaneous apoptosis in freshly isolated PBL are significantly elevated in the groups of elderly and young subjects.…”

Section: Discussioncontrasting

confidence: 59%

Antioxidant micronutrients improve intrinsic and UV-induced apoptosis of human lymphocytes particularly in elderly people

Ge²,

Zhang

et al. 2011

The Journal of nutrition, health and aging

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Section: Discussioncontrasting

confidence: 59%

Antioxidant micronutrients improve intrinsic and UV-induced apoptosis of human lymphocytes particularly in elderly people

Ge²,

Zhang

et al. 2011

The Journal of nutrition, health and aging

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“…The techniques for the quantification of total amount of cfDNA include fluorescence-based methods (such as Hoechst dye and PicoGreen staining), spectrophotometric-based methods (ultraviolet spectrometry) or quantitative real-time polymerase chain reaction (PCR) methods (such as SYBR Green and TaqMan) [35, 49, 50]. The study with plasma samples collected from 10 non-small cell lung cancer patients compared PicoGreen staining to real-time PCR methods for the quantification of cfDNA [51].…”

Section: Technologies For Cfdna Analysismentioning

confidence: 99%

Testing for oncogenic molecular aberrations in cell-free DNA-based liquid biopsies in the clinic: are we there yet?

Polívka

Pešta

Janků

2015

Expert Review of Molecular Diagnostics

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Summary The optimal choice of cancer therapy depends upon analysis of the tumor genome for druggable molecular alterations. The spatial and temporal intratumor heterogeneity of cancers creates substantial challenges, as molecular profile depends on time and site of tumor tissue collection. To capture the entire molecular profile, multiple biopsies from primary and metastatic sites at different time points would be required, which is not feasible for ethical or economic reasons. Molecular analysis of circulating cell-free DNA offers a novel, minimally invasive method that can be performed at multiple time-points and plausibly better represents the prevailing molecular profile of the cancer. Molecular analysis of this cell-free DNA offers multiple clinically useful applications, such as identification of molecular targets for cancer therapy, monitoring of tumor molecular profile in real time, detection of emerging molecular aberrations associated with resistance to particular therapy, determination of cancer prognosis and diagnosis of cancer recurrence or progression.

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“…Due to the high degree of fragmentation as well as the small fraction of ctDNA within the cfDNA, the analysis of ctDNA is challenging and requires highly sensitive techniques. Classical methods of analysis include qRT-PCR, fluorescence and spectrophotometric approaches [76][77][78]. Digital droplet PCR has been developed as a high sensitive tool to detect ctDNA [79].…”

Section: Circulating Tumour Dna (Ctdna)mentioning

confidence: 99%

Liquid Biopsy for Colorectal Cancer Screening, A Modern Approach for Patients Stratification and Monitoring

Ginghină¹,

Nițipir²

2018

Colorectal Cancer - Diagnosis, Screening and Management

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Despite great advances have been made in oncologic approaches, the morbidity and mortality caused by colon cancer are still overwhelming. Particularly, the intra-and inter-tumour heterogeneity makes accurate sampling challenging and often leads to failure of even modern therapeutic strategies. Moreover, tumour molecular genotype can suffer alterations over time, triggering suboptimal therapeutic outcomes as a result of irrelevant information provided by histological biopsies. Daily, tumour cells shed into the bloodstream at the early stages of the disease. These circulating tumour cells (CTCs) can be detected and analysed after enrichment, providing this way valuable information in real time. Furthermore, apoptotic and/or necrotic tumour cells discharge DNA fragments into the circulating bloodstream. Elevated levels of these so-called circulating tumour DNA (ctDNA) fragments can be identified in the peripheral blood of patients as compared to healthy individuals. In this view, the detection and characterization of the CTCs and ctDNA are a real-time "liquid biopsy" that has been developed for accurate tumour monitoring and molecular characterization. This modern non-invasive analytical approach allows consecutive sampling to monitor CTC number and tumour genetic changes over time without the need of tissue biopsy. Consequently, "liquid biopsies" can be used to screen for cancer, stratify patients to the optimum treatment and to monitor the patient's response to treatment or identify treatment resistance. This chapter offers an overview of the following approaches with respect to liquid biopsies: CTCs and ctDNA. Some of the analytical techniques and challenges in the detection of these rare events will also be presented here.

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The Origin of Elevated Levels of Circulating DNA in Blood Plasma of Premature Neonates

Cited by 24 publications

References 12 publications

Antioxidant micronutrients improve intrinsic and UV-induced apoptosis of human lymphocytes particularly in elderly people

Antioxidant micronutrients improve intrinsic and UV-induced apoptosis of human lymphocytes particularly in elderly people

Testing for oncogenic molecular aberrations in cell-free DNA-based liquid biopsies in the clinic: are we there yet?

Liquid Biopsy for Colorectal Cancer Screening, A Modern Approach for Patients Stratification and Monitoring

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