The origin of methylglyoxal in New Zealand manuka (Leptospermum scoparium) honey

Adams, Christopher J.; Manley‐Harris, Merilyn; Molan, Peter C.

doi:10.1016/j.carres.2009.03.020

Cited by 248 publications

(224 citation statements)

References 11 publications

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“…The unique manuka factor (UMF) is used to grade this honey and indicates the presence of dihydroxyacetone, leptosperin and variable amounts of MGO [8]. Manuka honey with a UMF > 10 is used for therapeutic purposes and has a MGO content of ≥ 263 mg/kg and is sterilised using gamma radiation [7,9].…”

Section: Introductionmentioning

confidence: 99%

How methylglyoxal kills bacteria: An ultrastructural study

Rabie

Serem

Oberholzer

et al. 2016

Ultrastructural Pathology

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Antibacterial activity of honey is due to the presence of methylglyoxal (MGO), H 2 O 2 , bee defensin as well as polyphenols. High MGO levels in manuka honey are the main source of antibacterial activity. Manuka honey has been reported to reduce the swarming and swimming motility of Pseudomonas aeruginosa due to de-flagellation. Due to the complexity of honey it is unknown if this effect is directly due to MGO. In this ultrastructural investigation the effects of MGO on the morphology of bacteria and specifically the structure of fimbriae and flagella were and E. coli morphology was then evaluated. At 0.5 mM MGO, bacteria structure was unaltered.For both bacteria at 1 mM MGO fewer fimbriae were present and the flagella were less or absent. Identified structures appeared stunted and fragile. At 2 mM MGO fimbriae and flagella were absent while the bacteria were rounded with shrinkage and loss of membrane integrity.Antibacterial MGO causes alterations in the structure of bacterial fimbriae and flagella which would limit bacteria adherence and motility.

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Section: Introductionmentioning

confidence: 99%

How methylglyoxal kills bacteria: An ultrastructural study

Rabie

Serem

Oberholzer

et al. 2016

Ultrastructural Pathology

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“…Also its antifungal activity against Candida sp. [37] or Leptospermum scoparium [38] is documented. According to its in vivo studies; honey topical application is recommended in difficult surgical wounds such as burns [39]; chronic leg wounds [25]; venous leg ulcers [40] or diabetic foot lesions [41].…”

Section: Discussionmentioning

confidence: 99%

Honey and Medicinal Plants in the Management of Certain Surgical Bovine Clow Affections

Ma¹,

As²,

Mm³

et al. 2017

IJCAM

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Owing to the worldwide spread of bacterial antimicrobial resistance, it is of great concern to search for antimicrobial agents of natural origin. Since the antimicrobial activity of honey was widely documented, the study aimed to evaluate its use comparing with certain medicinal plants extracts as surgical dressing of bovine clow affections. Aqueous extracts of Thymus vulgaris (T. vulgaris), Matricaria chamomilla (M. chamomilla) and Origanum vulgare (O. vulgare) were prepared to be used in vitro and in vivo studies in this work. Clinical aerobic or anaerobic bacterial strains were isolated from some clinical surgical bovine clow affections, and were used to determine the minimal inhibitory concentrations (MICs) of the prepared extracts against these pathogens.The study concluded that all tested aerobic bacterial strains were inhibited with 10 % of all tested extracts, while the anaerobic strains were inhibited with 10 % of T. vulgaris and 15 % of both O. vulgare and M. chamomilla extracts. According the achieved MICs values, lotions and ointments of the entended medicinal plants were made to be used during the in vivo study as follow: 20 lactating dairy cows suffering from different surgical clow affections were classified to equal four groups (A -D) which were managed with surgical dressing and received: honey (A): T. vulgaris (B), M. chamomilla: (C) and O. vulgare: (D) extracts with their MICs. All cows of groups A & B (no = 10)as well as one cow from group (C) showed complete healing by the day 30 while, none of group (D).The study concluded that with the alternative medical trends, application of honey -as it is -in surgical dressing of bovine digital dermatitis or inter digital necrobacillosis is the most effective valuable economic tool among the four materials studied for its superurity and feasibility followed by the use of T. vulgaris extract 10% in lotion and ointment preparation.

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“…In addition, dihydroxyacetone (DHA) and methylglyoxal (MGO) (Figure 1) are solely derived from L. scoparium nectar in New Zealand honeys [12,13]. Dihydroxyacetone is present in L. scoparium nectar, converting non-enzymatically and irreversibly to MGO in the acidic environment of a ripened honey solution [13].…”

Section: Introductionmentioning

confidence: 99%

“…Dihydroxyacetone is present in L. scoparium nectar, converting non-enzymatically and irreversibly to MGO in the acidic environment of a ripened honey solution [13]. This conversion is non-stoichiometric [14,15] due to the presence of side reaction pathways in the honey.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence: A Novel Method for Determining Manuka Honey Floral Purity

Stephens¹,

Loomes²,

Braggins³

et al. 2017

Honey Analysis

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Manuka honey, harvested from Leptospermum scoparium, is New Zealand's most recognised honey type and commands a premium due to health-related beneits. However, the plant's distribution, relative to other species lowering simultaneously, allows honeybees to incorporate alternative nectars into the honey. Melissopalynological analysis in New Zealand is often unrepresentative due to the presence of many pollen-bearing sources; consequently, alternative means of categorising manuka honey were examined. RP-HPLC revealed that manuka honey contains distinct compounds, of which were relatively enriched and not present in the other New Zealand monoloral honeys. These main candidate compounds were isolated and have been described by mass spectrometry and nuclear magnetic resonance, synthesised to conirm structure, and as standards. These compounds, Leptosperin and Lepteridine, are a methyl syringate glycoside and pteridine derivative, respectively. Examination of these compounds revealed unique luorescence signatures, this luorescence could be detected in manuka honey samples the signal used to conirm that a honey was solely or predominantly consisted of L. scoparium nectar. Commercial manuka honeys were assessed by traditional analytical techniques, and comparisons were made with luorescence signature; the luorescence technique determined the authenticity of the honeys accurately.

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The origin of methylglyoxal in New Zealand manuka (Leptospermum scoparium) honey

Cited by 248 publications

References 11 publications

How methylglyoxal kills bacteria: An ultrastructural study

How methylglyoxal kills bacteria: An ultrastructural study

Honey and Medicinal Plants in the Management of Certain Surgical Bovine Clow Affections

Fluorescence: A Novel Method for Determining Manuka Honey Floral Purity

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