The origin of ova and follicle cells from the germinal epithelium of the ovary of the albino rat as demonstrated by selective intravital staining with India ink

Abstract

TWO PLATES (TWELVE FIGURES)Since the early work ('00) of von Winiwarter describing postfetal ovogenesis in rabbit and man, several methods have been employed to test the validity of the concept.By selection of closely graded series of stages, numerous investigators httve introduced evidence of the postpartum formation of ova. These reports have been critically reviewed by Kingsbury ( '38). One of these, Arai ('20), after making extensive counts of the total number of ova present at different ages in rats, concluded that ovogenesis was a continuous process. Allen ( '231, using mouse ovaries produced evidence of the cyclic nature of the process, initiated by mitoses in the germinal epithelium. Bagg and Papanicolaou ( '24) were able to accelerate ovarian activity in guinea pigs by irradiation of the thyroid gland, and found ova, follicle cells, interstitial and lutein cells all were derived from the germinal epithelium. Allen and Creadick ('37) and Allen, Smith and Gardner ('37) by using colchicine in mice more clearly demonstrated mitosis in the germinal epithelium and found convincing evidence of cyclic proliferation of ova from the germinal epithelium, the peak appearing at estrus. Schmidt and Hoffman ( '41) using this technique demonstrated such activity in guinea pig ovaries, only part of which was thought to be concerned in ovogenesis.In another approach Long ('40) reported abundant growth of germinal epithelium in tissue cultures of bits of mouse ovaries. He further demonstrated the formation of ova and follicles from this cultured epithelium.Marx ('41) using rats' ovaries stimulated by injection of gonadotropic extracts, found no evidence of proliferation of ova from the germinal 'A preliminary report of this investigation appeared as an abstract read by title at the meet-

Ovarian neoplasia in the Sprague-Dawley rat.

“…There are two obvious suggestions: inclusions represent a storage of products of intracellular synthesis and the accumulation of an absorbed extracellular material. Ovarian rhesothelial cells are known to be capable of phagocytosis (17). Whatever their origin, these inclusions would at present appear of diagnostic value for tubular adenomas in rats.…”

Conserved form and function of the germinal epithelium through 500 million years of vertebrate evolution

“…The primordial germ cells, which become spermatogonia in the testes and oogonia in the ovaries, are the precursors of the gametes. The somatic epithelial cells are the precursors of the Sertoli cells in the testis and the follicular (granulosa) cells in the ovary (Latta and Pederson, 1944;Chiquoine, 1954;Niewkoop and Sutasurya, 1979;Tanimura and Iwasawa, 1991;Sawyer et al, 2002;Miura et al, 2003).…”

Extragonadal primordial germ cells or placental progenitor cells?

“…This view goes back to the latter part of the 19th century (Waldeyer, 1870). Despite an array of subsequent challenges, beginning in 1923 and continuing to the present (Adsell, 1964;Allen, 1923;Allen and Creadick, 1937;Bukovsky et al, 2004Bukovsky et al, , 2005Butcher, 1927;Duke, 1941Duke, , 1944Hargitt, 1930;Johnson et al, 2004;Latta and Pederson, 1944;Nikura et al, 2009;Pliske, 1938;Risley, 1934;White et al, 2012), Waldeyer's view has predominated. However, in recent years, the possibility that both male and female germ cells arise from a common origin in the germinal epithelia has finally gained traction (Grier et al, 2016;Tilly et al, 2009).…”