The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis

Vandesande, Frans; Dierickx, K; Mey, J. De

doi:10.1007/bf00220167

Cited by 284 publications

(73 citation statements)

References 15 publications

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“…Proenkephalinand [Met]enkephalin-immunoreactive fibers and terminals were found in the external median eminence and were concentrated in the periphery of the neurohypophysis. We found a similar distribution for oxytocin in these tissues as has been reported by others (19,25). It has been reported that another opioid peptide, dynorphin, is present in vasopressin-containing magnocellular neurons (9,13).…”

Section: Discussionsupporting

confidence: 91%

“…The general distribution of proenkephalin IR and [Met]enkephalin IR in the hypothalamo-neurohypophyseal system was superimposable on the distribution of oxytocin. Proenkephalinand [Met]enkephalin-immunostained cell bodies were visualized in the dorsal part of the supraoptic nuclei and in the lateral peripheral part of the paraventricular nuclei, in the same areas as are found oxytocinergic cell bodies (19,25). Proenkephalinand [Met]enkephalin-immunoreactive fibers and terminals were found in the external median eminence and were concentrated in the periphery of the neurohypophysis.…”

Section: Discussionmentioning

confidence: 63%

“…The magnocellular neurons of the hypothalamo-neurohypophyseal system contain a wide variety of peptide hormones, including vasopressin, oxytocin, cholecystokinin, and corticotropin-releasing factor (19,(24)(25)(26). The hypothalamus is also rich in opiate peptides (8)(9)(10)(11)(12)(13).…”

Section: Discussionmentioning

confidence: 99%

“…Anti-oxytocin--antiserum was similarly treated with vasopressin-Sepharose 4B. Complete details on the preparation and specificity of these antisera have been reported (19).…”

mentioning

confidence: 99%

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Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons.

Vanderhaeghen¹,

Lotstra²,

Liston³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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The distribution of proenkephalin and [Met] Adsorbed antisera against oxytocin and vasopressin were generous gifts from F. Vandesande. Briefly, anti-vasopressin antiserum was adsorbed by treatment with oxytocin coupled to Sepharose 4B beads. Anti-oxytocin--antiserum was similarly treated with vasopressin-Sepharose 4B. Complete details on the preparation and specificity of these antisera have been reported (19).For the production of the anti-proenkephalin antiserum, a protein composed of the first 77-amino-acid residues of adrenal proenkephalin was purified from the adrenal medulla (16). The purified protein was injected into the popliteal lymph nodes of adult male Bourgogne rabbits. Antiserum obtained 2 wk after the third injection was found to be suitable for this study. Crossreactivity in a radioimmunoassay using this antiserum was less than 0.001% for the following peptides: [Met] 5139The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

“…Anti-oxytocin--antiserum was similarly treated with vasopressin-Sepharose 4B. Complete details on the preparation and specificity of these antisera have been reported (19).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons.

Vanderhaeghen¹,

Lotstra²,

Liston³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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“…Vasopressin, but not oxytocin, is synthesized in small cell bodies of neurons in the parvocellular division of the PVN that project to the external zone of the median eminence (Vandesande et al, 1977). Release of vasopressin into the hypophysial portal circulation results in the potentiation of corticotropin-releasing factor-induced adrenocorticotropin secretion from the anterior pituitary, which in turn regulates corticosterone secretion by the adrenal gland (Gillies et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Neurohormones: oxytocin, vasopressin and related peptides – structure, genes, receptors, and evolution

Kochman¹

2013

J. Anim. Feed Sci.

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Considered biologically as well as philosophicallyABSTRACT. Oxytocin (OT) and vasopressin (VP), and all related hormones, consist of nine amino acids with cysteine residues in positions 1 and 6 that form a six-amino acid cyclic part, and of a C-terminal glycine in α-amidated form. These neuropeptides are classified into oxytocin and vasopressin families based on the amino acid residue at position 8. OT-like and VP-like peptides are present in every vertebrate species. These peptides are a very ancient family of hormones having representatives in diverse species of invertebrates. Invertebrates have either a vasopressin-family peptide or an oxytocin-family peptide, whereas bony fishes, the ancestors of land vertebrates, have both isotocin and vasotocin. Presently, two evolutionary structural lineages have been proposed: an isotocin-mesotocin-OT line, associated with reproductive functions, and a vasotocin-VP line participating in water homeostasis. The ancestral gene encoding the precursor protein has been present in the animal genome for a period exceeding 500 million years of evolution. The exceptionally high stability of this structure of nine-amino acid peptides during the entire process of evolution suggests very powerful selective pressure, possibly by evolution together with respective receptors and specific processing enzymes. A novel gene with a distinct function and expression appeared during evolution through duplication of an ancestral gene. The synteny and order of genes in the neurohypophysial hormone gene locus are conserved in the lamprey, elephant shark, coelacanth, and tetrapods, but disrupted in teleost fishes presumably due to the rearrangements facilitated by a whole-genome duplication event in the teleost fish ancestor. 284 Evolution of neurohormones

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Organization of the human paraventricular hypothalamic nucleus

et al. 2000

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The cyto- and chemoarchitecture of the human paraventricular hypothalamic nucleus (Pa) was studied with the aid of three-dimensional computer reconstruction. The adult human Pa is a vertically elongated structure that abuts the wall of the third ventricle (3V) medially and is indented dorsolaterally by the descending fornix. Chemoarchitecture revealed the following five subnuclei in the human Pa. The most prominent of these is the magnocellular subnucleus (PaM) occupying the ventrolateral quadrant of the Pa and comprised of a concentration of large arginin-vasopressin (AVP)- and acetylcholinesterase (AChE)-positive cells, and small calbindin (Cb)-positive neurons. Rostrally, the PaM is succeeded by the small anterior parvicellular subnucleus (PaAP), which contains small AChE-, AVP- and tyrosin hydroxylase (TH)-positive cells. Dorsal to the PaM is found the dorsal subnucleus (PaD), containing large spindle-shaped TH-, oxytocin (OXY)-, and AChE-positive cells, as well as a population of small Cb-positive neurons. Abutting the wall of the 3V and medial to PaM and PaD is the parvicellular subnucleus (PaP). The PaP contains small cells immunoreactive for corticotropin-releasing factor (CRF), neuromedin K receptor (NK3), and nonphosphorylated neurofilament protein (SMI32). The posterior subnucleus (PaPo) is situated posterior to the descending column of the fornix; it replaces all above-mentioned subdivisions caudally, and is a chemoarchitectonic amalgam that includes dispersed large AChE-, OXY-, AVP- and TH-positive cells, as well as small NK3-, CRF-, SMI32- and Cb-immunoreactive neurons. The present findings suggest that the human PaM and PaD are homologues to the magnocellular subnuclei of the rat Pa, whereas the human PaP and PaPo correspond to the rat medial parvicellular and posterior subnuclei, respectively.

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The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis

Cited by 284 publications

References 15 publications

Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons.

Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons.

Neurohormones: oxytocin, vasopressin and related peptides – structure, genes, receptors, and evolution

Organization of the human paraventricular hypothalamic nucleus

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