The origin of the volatile metabolites found in mastitis milk

Hettinga, Kasper; Valenberg, H.J.F. van; Lam, T.J.G.M.; Hooijdonk, A.C.M. van

doi:10.1016/j.vetmic.2009.01.016

Cited by 40 publications

(39 citation statements)

References 5 publications

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“…On the other hand, excessive sesquiterpenoids can lead to toxic effects, such as producing spastic neurotoxins and pro-in ammatory factors [51]. Hettinga et al [52] found that the toxic volatile metabolites, dimethyl sul de, in milk from mastitis cows mainly originated from pathogens, such as Staphylococcus aureus, Streptococcus uberis and E.coli. It is speculated that the increase of 10beta-Hydroxy-6beta-isobutyrylfuranoeremophilane in the rumen may be a result of the increased rumen pathogens or the damage of blood-milk barrier.…”

Section: Discussionmentioning

confidence: 99%

Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Wang

Nan²,

Zhao³

et al. 2020

Preprint

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Background: With high prevalence and complex etiology, bovine mastitis (BM) often causes serious damage to dairy cows’ health and milk quality. Although the shift in milk has been the main research interest on mastitis, recent studies have suggested that gastrointestinal microorganism has a crucial effect on the inflammation of the tissues outside the gut, such as mammary gland. However, studies related to the variation of rumen inner-environment during mastitis were limited. This study compared the differences of rumen microorganisms and metabolites under different udder health conditions, in order to investigate the correlation between udder health status and rumen microbiome structure and metabolites activity. Based on the somatic cell counts (SCC), California mastitis test (CMT) parameters and clinical symptoms of mastitis, 60 lactating Holstein dairy cows with similar body conditions (excepted for udder health condition) were divided into 3 groups (n = 20 per group): healthy (H) group, subclinical mastitis (SM) group and clinical mastitis (CM) group. Lactation and rumen fermentation parameters were detected, and rumen microbiota and metabolites were analyzed via 16S rRNA amplicon sequencing and untargeted metabolomics, respectively. Results: As the degree of mastitis increased, milk SCC and milk protein were significantly increased (P < 0.01). Milk yield, milk fat and lactose (P < 0.01), and rumen lactic acid (LA) (P < 0.01), acetate, propionate, butyrate, valerate (P < 0.001), total volatile fatty acids (TVFAs) concentrations (P < 0.01) and pH value (P = 0.024) were significantly decreased. In the rumen of CM cows, the significantly increased pathogens related to intestinal and oral inflammation, such as g__Lachnospiraceae_UCG-006 (P = 0.03), g__Moraxella and g__norank_f__Neisseriaceae (P < 0.01), etc., were accompanied by a significant increase in 12-oxo-20-dihydroxy-leukotriene B4 and 10beta-Hydroxy-6beta-isobutyrylfuranoeremophilane (P < 0.01). Meanwhile, in the rumen of SM cows, the g__Ruminiclostridium_9 (P = 0.04) and g_Enterorhabdus (P = 0.01) were increased with methenamine, 5-Hydroxymethyl-2-furancarboxaldehyde (5-HMF) and 6-Methoxymellein (P < 0.01). During mastitis, the decrease of short-chain fatty acids (SCFAs)-producing bacteria and intestinal probiotics in rumen, including g__Prevoterotoella_1 and g__Bifidobacterium (P = 0.03), etc., were significantly reduced, accompanied by a reduction in 2-Phenylbutyric acid (2-PBA) (P < 0.01).Conclusion: This study suggested that the microflora and metabolites associated with pathogen invasion, inflammatory response and rumen wall damage in the rumen of cows suffered mastitis were prominent.

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Section: Discussionmentioning

confidence: 99%

Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Wang

Nan²,

Zhao³

et al. 2020

Preprint

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“…For example, specific volatile metabolites detected in mastitis milk samples are formed by and are specific to each pathogen (Hettinga et al 2009). Other components are more or less affected depending on the pathogen.…”

Section: Changes In Milk Associated With a Rise In The Scc And Variatmentioning

confidence: 99%

“…This requires an increase in endothelial permeability, which induces not only the transfer of PMN from blood to milk but also other blood components such as bovine serum albumin (BSA). Lipolysis in milk can be explained by the disturbance of the milk-blood barrier which allows the transfer of esterases from blood to mastitic milk (Hettinga et al 2009). Another example of increased permeability is found in transferrin (an iron transporter), which is not produced in the mammary gland of ruminant (Sanchez et al 1992) but comes from blood serum (OllivierBousquet 1998) and rapidly increases in E. coli mastitis .…”

Section: Increased Endothelial Permeabilitymentioning

confidence: 99%

Mastitis impact on technological properties of milk and quality of milk products—a review

Maréchal

Thiéry²,

Vautor

et al. 2011

Dairy Science & Technol.

150

139

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The consequences of mastitis on the technological properties of milk and on the quality of milk products are widely reported in the literature. Besides, recent advances have shed light on the mechanisms involved in the udder response and subsequent milk changes in mastitis cases. This review gives an update on the literature regarding the impact of mastitis on milk composition and processing properties and collates recent data regarding the mechanisms involved in mastitis effects. It is an attempt to link field observations and experimental studies in order to better understand how mastites affect so dramatically the technological properties of milk. Both bovine and small ruminant milks are considered and a special emphasis is given on the role of staphylococci, streptococci, and Escherichia coli, the most common causative agents of mastitis.

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“…However, little information exists about the changes this may induce in the biochemical composition of human milk. In contrast, it is well known that, in bovine mastitis, the metabolism of the infecting bacteria changes the volatile compound profile [14]. …”

Section: Introductionmentioning

confidence: 99%

Mastitis Modifies the Biogenic Amines Profile in Human Milk, with Significant Changes in the Presence of Histamine, Putrescine and Spermine

et al. 2016

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Biogenic amines (BAs) are low molecular weight nitrogenous organic compounds with different biological activities. Putrescine, spermidine and spermine are essential for the development of the gut and immune system of newborns, and are all found in human milk. Little is known, however, about the role of histamine, tyramine or cadaverine in breast milk. Nor is it known whether mastitis alters the BA composition of milk. The BA profile of human milk, and the influence of mastitis on BA concentrations, were therefore investigated. Putrescine, spermidine and spermine were the main BAs detected. In mastitis-affected milk, the concentrations of putrescine, spermine and histamine were higher.

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The origin of the volatile metabolites found in mastitis milk

Cited by 40 publications

References 5 publications

Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Mastitis impact on technological properties of milk and quality of milk products—a review

Mastitis Modifies the Biogenic Amines Profile in Human Milk, with Significant Changes in the Presence of Histamine, Putrescine and Spermine

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