The Osmotic Fragility of Erythrocytes in Experimental Malaria *

Fogel, Bernard J.; Shields, Charles E.; Doenhoff, Albert E Von

doi:10.4269/ajtmh.1966.15.269

Cited by 52 publications

(17 citation statements)

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“…Il est impossible de séparer complètement les hématies parasitées des hématies norma les et des réticulocytes ; toutefois les hématies parasitées et les réticulocytes de densité plus faible, constituent les couches supérieures des culots globulaires après centrifu gation donnant ainsi des fractions enrichies en hématies parasitées ou en réticulo cytes (Fogel 1966, Dunn 1966. Ces couches supérieures sont donc mélangées, lavées comme ci-dessus et regroupées pour déterminations.…”

Section: Préparation Des Hématiesunclassified

“…La fragilité osmotique est accrue Fogel (1966), le transport du sodium, potassium (Dunn 1969) et des acides aminés (McCormick 1970) est modifié et une augmentation prononcée du contenu lipidique a été rapportée (Lawrence 1969). Ces altérations ont été retrouvées dans la plupart des cas à la fois dans les hématies parasitées et dans les hématies non parasitées des animaux infestés.…”

Section: Introductionunclassified

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Plasmodium chabaudi : Modification des acides sialiques de surface des hématies au cours de l’infestation

Fabia

Gattegno

et al. 1979

Ann. Parasitol. Hum. Comp.

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Résumé.Les acides sialiques de surface des hématies de souris infestées par le parasite Plasmodium chabaudi sont augmentés comparativement à ceux d'hématies de souris non infestées. Cette augmentation coïncide avec celle des réticulocytes circulants. Une augmen tation similaire des acides sialiques de surface est montrée par l'étude des réticulocytes induits par phénylhydrazine. De la même façon, les oses neutres et le fucose sont trouvés en quantité plus importante dans les glycopeptides trypsiques de surface desréticulocytes que dans les glycopeptides trypsiques de surface des hématies. Cette augmentation se retrouve dans les hématies de souris infestées par Plasmodium chabaudi, parallèlement à la valeur de la réticulocytémie. Ceci suggère la contribution significative des réticulocytes aux variations biochimiques observées des hématies infestées au cours de la malaria.

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Section: Préparation Des Hématiesunclassified

Section: Introductionunclassified

Plasmodium chabaudi : Modification des acides sialiques de surface des hématies au cours de l’infestation

Fabia

Gattegno

et al. 1979

Ann. Parasitol. Hum. Comp.

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“…He found that red blood cell sodium increased and red blood cell potassium decreased as the parasitemia increased, and postulated the existence of a circulating toxin that increased membrane permeability. Other investigators have postulated that a circulating factor may be present in malarial plasma, since the rate of hemolysis exceeds that which would be expected from the number of parasitized erythrocytes (7) and the osmotic fragility of nonparasitized cells is abnormal (8). Normal erythrocytes show a markedly shortened life span after transfusion into a malarious recipient (9,10).…”

Section: Introductionmentioning

confidence: 99%

Alterations of red blood cell sodium transport during malarial infection

Dünn¹

1969

J. Clin. Invest.

115

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A B S T R A C T Previous studies have suggested that malaria induces changes in erythrocytic membrane permeability and susceptibility to osmotic lysis. The present study investigated erythrocytic transport of sodium with cells from Rhesus monkeys infected with Plasmodium knowlesi. Red blood cell sodium concentration was significantly elevated in 37 parasitized animals (21.8 ±1.2 mM; mean +SEM), as compared to 23 control animals (10.0 +0.38 mM). The cellular sodium increased with the density of parasitemia and the cellular potassium decreased in proportion to the elevation of sodium. Nonparasitized as well as parasitized erythrocytes possessed this abnormality of cation metabolism. Effective chloroquine therapy reversed the changes over a period of 4 days.Active sodium outflux rate constants were depressed in animals with malaria (0.202 +0.012), as compared to controls (0.325 ±0.027). Passive sodium influx rate constants were higher in infected monkeys (0.028 +0.002) than in control animals (0.019 +0.002). The cross incubation of malarial plasma with normal red blood cells induced a 22% diminution in active sodium outflux but no changes were observed in sodium influx.It is concluded that malaria alters erythrocytic sodium transport in all erythrocytes. The elevated intracellular sodium concentration is the net result of decreased sodium outflux and increased sodium influx. The plasmodium organism or the affected host may produce a circulating substance that is deleterious to erythrocytic membrane cation transport.

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“…In addition, the biochemical abnormalities of the RBC and its membrane due to intracellular parasitism can increase hemolysis. (25). Further, lowered filterability (26) and increased fragility (27) have been reported in the erythrocytes of experimental animals afflicted by malaria.…”

Section: Methodsmentioning

confidence: 99%

Hematological Impairments in Recurrent Plasmodium Vivax Infected Patients

Selvam

Gunasekaran

1996

JJMSB

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SUMMARY:The hematological parameters were assayed in Plasmodium vivax patients with only one infection, two infections, three infections and more than three malarial infections during a period of six months. A steady fall in the levels of hemoglobin as well as packed cell volume (PCV) level was observed with increasing number of infections. The malarial patients showed a progressive decrease in RBC level with increasing number of attacks. The decrease in the hematological indices was statistically significant at all levels of parasitemia. There was a marked increase in the osmotic fragility of the malarial erythrocytes when compared to that of controls. During repeated malarial attacks, significant decrease in MCH (p<0.05) and MCHC (p<0.01) and increase in the MCV level (p <0.05) and Heinz body formation (p<0.001) were observed. Parasite density significantly influenced the fragility of the erythrocytes, Heinz body formation, MCV, MCH and MCHC levels. Thus, the erythrocytes of the patients repeatedly infected with Plasmodium vivax parasite are subjected to structural and functional impairment, ultimately culminating in anemia.

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The Osmotic Fragility of Erythrocytes in Experimental Malaria *

Cited by 52 publications

References 0 publications

Plasmodium chabaudi : Modification des acides sialiques de surface des hématies au cours de l’infestation

Plasmodium chabaudi : Modification des acides sialiques de surface des hématies au cours de l’infestation

Alterations of red blood cell sodium transport during malarial infection

Hematological Impairments in Recurrent Plasmodium Vivax Infected Patients

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