The osteogenic differentiation potentials of umbilical cord blood hematopoietic stem cells

Khorshied, Mervat M.; Gouda, Heba; Shaheen, Iman A.; Bolkeny, Tarek N. Al

doi:10.1007/s00580-010-1115-1

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…First, it has been reported that they have an osteogenic potential even equal to that of BM-MSCs [57,58] as proved by measurements of ALP activity, activation of osteogenic genes, Alizarin Red [59] and von Kossa stains, as well as by the expression of ECM proteins [58]. Furthermore, a study compared cord blood mononuclears with ESCs in regard to their ability to produce ectopic bone following intramuscular implantation of differentiated stem cell-collagen constructs.…”

Section: Bone Tissue Regenerationmentioning

confidence: 98%

Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells

Klontzas¹,

Kenanidis

Heliotis

et al. 2015

Expert Opinion on Biological Therapy

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MSCs from umbilical cords have to be more extensively studied and the mechanisms underlying their differentiation have to be clarified. To date, they seem to be an attractive alternative to BM-MSCs. However, further research with suitable scaffolds and growth factors as well as with novel scaffold fabrication and culture technology should be conducted before they are introduced to clinical practice and replace BM-MSCs.

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Section: Bone Tissue Regenerationmentioning

confidence: 98%

Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells

Klontzas¹,

Kenanidis

Heliotis

et al. 2015

Expert Opinion on Biological Therapy

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“…Alizarin Red staining detected osteoblastic differentiation in hAMSCs and hUC-MSCs (15, 16). Runt-related transcription factor 2 (RUNX2) is an early stage osteoblastic differentiation marker which important in osteogenesis.…”

Section: Introductionmentioning

confidence: 99%

Phenotype Characteristics and Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells Derived from Amnion Membrane (HAMSCs) and Umbilical Cord (HUC-MSCs)

Hendrijantini¹,

Hartono²

2019

Acta Inform Med

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Introduction: Human amnion membrane mesenchymal stem cells (hAMSCs) and human umbilical cord mesenchymal stem cells (hUC-MSCs) are potential, non invasive sources of stem cells used for bone tissue engineering. Phenotyping characterization is an extremely important consideration in the choice of the appropriate passage in order to maximize its osteogenic differentiation potential. Aim: To explore phenotype characteristics and compare osteogenic differentiation potential of hAMSCs and hUC-MSCs. Method: Isolation and culture were performed on hAMSCs and hUC-MSCs from a healthy woman in her 38 th weeks of pregnancy. CD90, CD105 and CD73 phenotype characterization was done in passage 4-7. An osteogenic differentiation examination of hAMSCs and hUC-MSCs with Alizarin red staining and RUNX2 expression was performed in the passage that had appropriate expressions of phenotype characteristics. Results: The expression of CD90 hUC-MSCs was higher than that of hAMSCs in all passages. CD105 hUC-MSCs was higher in passage 4-6, while CD105 hAMSCs was equal to that of hUC-MSCs in passage 7. CD73 hUC-MSCs was higher than hAMSCs in passage 4 and 5, while in passage 6 and 7 hAMSCs was higher than hUC-MSCs. There was a decrease in the number of CD90, CD105 and CD73 on hAMSCs and hUC-MSCs in passage 5, then determined as appropriate passage. Alizarin red staining examination showed calcium deposition and revealed no significant difference, but RUNX2 expression of hUC-MSCs was significantly higher than that for hAMSCs. Conclusion: Both hAMSCs and hUC-MSCs had phenotype characteristics of mesenchymal stem cell and showed ostegenic differentiation potential.

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The osteogenic differentiation potentials of umbilical cord blood hematopoietic stem cells

Cited by 2 publications

References 21 publications

Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells

Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells

Phenotype Characteristics and Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells Derived from Amnion Membrane (HAMSCs) and Umbilical Cord (HUC-MSCs)

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