The osteogenic response to chirality-patterned surface potential distribution of CFO/P(VDF-TrFE) membranes

Zhang, Jiamin; He, Xuzhao; Zhou, Zhiyuan; Chen, Xiaoyi; Shao, Jinchen; Huang, Danfeng; Dong, Lingqing; Lin, Jun; Wang, Huiming; Weng, Wenjian; Cheng, Kui

doi:10.1039/d2bm00186a

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…Dong et al created a chiral geometric topology with micron-scale ups and downs to promote the distribution of cell adhesion states according to the designed pattern, which enhanced the mechanical conduction of the cytoskeleton and finally regulated the osteogenic function of cells. At the same time, our previous studies have shown that the distribution of macroscopic surface potential chirality can also effectively improve the osteogenic ability of cells . However, for the current study, the mechanism by which pattern modulation of surface potential affects macrophage polarization and immune responses remains unclear.…”

Section: Introductionmentioning

confidence: 80%

“…The electroactive films were prepared as reported in previous studies. 12 The magnetic particles (10 wt % (CFO/P(VDF-TrFE)) were dispersed in N,N-dimethylformamide (DMF, Sinopharm Chemical Reagent Co. Ltd) and ultrasonically dispersed for 6 h. P(VDF-TrFE) powder was added and stirred for 2 h. Films were prepared on the chirality-patterned ITO substrate by the solution casting method. After heat treatment at 210 °C for 60 min, the CFO/ P(VDF-TrFE) films were obtained.…”

Section: Methodsmentioning

confidence: 99%

“…At the same time, our previous studies have shown that the distribution of macroscopic surface potential chirality can also effectively improve the osteogenic ability of cells. 12 However, for the current study, the mechanism by which pattern modulation of surface potential affects macrophage polarization and immune responses remains unclear. In practical applications, the implantation of biomaterials inevitably triggers some immune cell-mediated immune responses, and the immune responses play an important role in releasing pro-or anti-inflammatory factors to regulate angiogenesis and bone formation.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Macrophage Polarization on Chiral Potential Distribution of CFO/P(VDF-TrFE) Films

Zhou

Zhang

et al. 2023

ACS Biomater. Sci. Eng.

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Surface potentials of biomaterials have been shown to regulate cell fate commitment. However, the effects of chirality-patterned potential distribution on macrophage polarization are still only beginning to be explored. In this work, we demonstrated that the chirality-patterned potential distribution of CoFe2O4/poly(vinylidene fluoride-trifluoroethylene) (CFO/P(VDF-TrFE)) films could significantly down-regulate the M1 polarization of bone marrow-derived macrophages (BMDMs). Specifically, the dextral-patterned surface potential distribution simultaneously up-regulated the expression of M2-related markers of BMDMs. The results were attributed to the sensitive difference of integrin subunits (α5β1 and αvβ3) to the dextral- and sinistral-patterned surface potential distribution, respectively. The interaction difference between the integrin subunits and surface potential distribution altered the cell adhesion and cytoskeletal structure and thereby the polarization behavior of BMDMs. This work, therefore, emphasizes the importance of chirality of potential distribution on cell behavior and provides a new strategy to regulate the immune response of biomaterials.

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Section: Introductionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of Macrophage Polarization on Chiral Potential Distribution of CFO/P(VDF-TrFE) Films

Zhou

Zhang

et al. 2023

ACS Biomater. Sci. Eng.

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“…To enhance its ferroelectric and piezoelectric attributes, researchers (Nunes-Pereira et al, 2015) introduced the trifluoroethylene monomer (TrFE) into PVDF, resulting in the copolymer Poly(vinylidene fluoridetrifluoroethylene) (P(VDF-TrFE)), which exhibit improved charge accumulation and storage capabilities, enabling the acquisition of a permanent surface potential. P(VDF-TrFE) is capable of carrying surface charge and has been demonstrated to promote osteogenesis in MSCs in vitro (Luo et al, 2022;Zhang et al, 2022), enhance bone repair in vivo (Gimenes et al, 2019;Zhang et al, 2022), and facilitate macrophage polarization (Dai et al, 2021). The application of an external power source through ITO planar microelectrodes can impart a continuous electrical stimulation to cells or tissues, thereby influencing their morphology and function.…”

Section: Introductionmentioning

confidence: 99%

“…P(VDF-TrFE) is capable of carrying surface charge and has been demonstrated to promote osteogenesis in MSCs in vitro ( Luo et al, 2022 ; Zhang et al, 2022 ), enhance bone repair in vivo ( Gimenes et al, 2019 ; Zhang et al, 2022 ), and facilitate macrophage polarization ( Dai et al, 2021 ). The application of an external power source through ITO planar microelectrodes can impart a continuous electrical stimulation to cells or tissues, thereby influencing their morphology and function.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the impact of planar microelectrodes on macrophage-mediated mesenchymal stem cell osteogenesis

Chen,

Xu,

Huang

et al. 2024

Front. Cell Dev. Biol.

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Osseointegration commences with foreign body inflammation upon implant placement, where macrophages play a crucial role in the immune response. Subsequently, during the intermediate and late stages of osseointegration, mesenchymal stem cells (MSCs) migrate and initiate their osteogenic functions, while macrophages support MSCs in osteogenesis. The utilization of ferroelectric P(VDF-TrFE) covered ITO planar microelectrodes facilitated the simulation of various surface charge to investigate their effects on MSCs’ osteogenic differentiation and macrophage polarization and the results indicated a parabolic increase in the promotional effect of both with the rise in piezoelectric coefficient. Furthermore, the surface charge with a piezoelectric coefficient of −18 exhibited the strongest influence on the promotion of M1 polarization of macrophages and the promotion of MSCs’ osteogenic differentiation. The impact of macrophage polarization and MSC osteogenesis following the interaction of macrophages affected by surface charge and MSC was ultimately investigated. It was observed that macrophages affected by the surface charge of −18 piezoelectric coefficient still exerted the most profound induced osteogenic effect, validating the essential role of M1-type macrophages in the osteogenic differentiation of MSCs.

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Remodel Heterogeneous Electrical Microenvironment at Nano‐Scale Interface Optimizes Osteogenesis by Coupling of Immunomodulation and Angiogenesis

Tang,

Fan,

Sun

et al. 2024

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Immunomodulation is essential for implants to regulate tissue regeneration, while bioelectricity plays a fundamental role in regulating immune activities. Under natural preferences, the bone matrix electrical microenvironment is heterogeneous in the nanoscale, which provides fundamental electrical cues to regulate bone immunity and regenerative repair. However, remodeling bone nanoscale heterogeneous electrical microenvironment remains a challenge, and the underlying immune modulation mechanism remains to be explored. In this research, in situ discretely distributed nano‐heterojunctions are constructed on titanium oxide nanofibers to mimic the heterogeneous electrical microenvironment exhibited by bone collagen fibers. The material is identified to directly regulate calcium ion channeling for anti‐inflammatory polarization of macrophages. Surprisingly, the highly biomimetic heterogeneous electrical microenvironment can induce a pro‐angiogenic phenotypic transformation of macrophages, leading to enhanced neo‐vascularization at the early stage of osteogenesis. Mechanistic exploration identifies that PI3K signaling pathway‐mediated FGF2 secretion may partially explain for strengthened coupling of immunomodulation and angiogenesis, which optimizes subsequent bone regeneration. These findings highlight the significance of biomimetic heterogeneous electrical cues on immune‐modulation and provide a design principle for future electroactive implant materials.

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The osteogenic response to chirality-patterned surface potential distribution of CFO/P(VDF-TrFE) membranes

Abstract: Piezoelectric poly(vinylidene fluoride-trifluoroethylene) has demonstrated an ability to promote osteogenesis, and the biomaterials with a chirality-patterned topological surface could enhance cellular osteogenic differentiation. In this work, we created a chirality-patterned...

Cited by 9 publications

References 33 publications

Regulation of Macrophage Polarization on Chiral Potential Distribution of CFO/P(VDF-TrFE) Films

Regulation of Macrophage Polarization on Chiral Potential Distribution of CFO/P(VDF-TrFE) Films

Investigation of the impact of planar microelectrodes on macrophage-mediated mesenchymal stem cell osteogenesis

Remodel Heterogeneous Electrical Microenvironment at Nano‐Scale Interface Optimizes Osteogenesis by Coupling of Immunomodulation and Angiogenesis

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