The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence

Lenz, Luana Suéling; Wink, Márcia Rosângela

doi:10.1007/s13577-023-00925-3

Cited by 6 publications

(1 citation statement)

References 115 publications

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“…Infinite cell lines are an irreplaceable tool for many biological assays [11][12][13]. Therefore, several approaches were developed to convert primary cells, isolated from different tissues, into long-lived cultures with consistent biological functions [14][15][16]. In this study we created an immortalized adipose tissue-derived MSC culture via ectopic expression of the human TERT gene, similar to what had been previously described for bone marrow stromal cells [17].…”

Section: Discussionmentioning

confidence: 96%

Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling

Primak,

Kalinina,

Skryabina

et al. 2024

IJMS

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Multipotent mesenchymal stromal cells (MSCs) integrate hormone and neuromediator signaling to coordinate tissue homeostasis, tissue renewal and regeneration. To facilitate the investigation of MSC biology, stable immortalized cell lines are created (e.g., commercially available ASC52telo). However, the ASC52telo cell line has an impaired adipogenic ability and a depressed response to hormones, including 5-HT, GABA, glutamate, noradrenaline, PTH and insulin compared to primary cells. This markedly reduces the potential of the ASC52telo cell line in studying the mechanisms of hormonal control of MSC’s physiology. Here, we have established a novel immortalized culture of adipose tissue-derived MSCs via forced telomerase expression after lentiviral transduction. These immortalized cell cultures demonstrate high proliferative potential (up to 40 passages), delayed senescence, as well as preserved primary culture-like functional activity (sensitivity to hormones, ability to hormonal sensitization and differentiation) and immunophenotype up to 17–26 passages. Meanwhile, primary adipose tissue-derived MSCs usually irreversibly lose their properties by 8–10 passages. Observed characteristics of reported immortalized human MSC cultures make them a feasible model for studying molecular mechanisms, which regulate the functional activities of these cells, especially when primary cultures or commercially available cell lines are not appropriate.

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Section: Discussionmentioning

confidence: 96%

Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling

Primak,

Kalinina,

Skryabina

et al. 2024

IJMS

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show abstract

Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

Zhang,

Xu,

Huang

et al. 2024

Anim Models and Exp Med

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BackgroundA stable and standardized source of mesenchymal stem cells is a prerequisite for bone repair tissue engineering research and application. We aimed to establish a stable cell line of bone marrow mesenchymal stem cells from New Zealand rabbits and explore their osteogenic differentiation capacity.MethodsPrimary rabbit bone marrow mesenchymal stem cells (RBMSCs) were isolated and immortalized via retroviral expression of SV40 Large T antigen (LTA). To assess the osteogenic differentiation capacity of the cells in vitro, we studied the alkaline phosphatase (ALP) expression level and calcium deposition in bone morphogenetic protein 9 (BMP9)‐induced immortalized cells using ALP staining and quantification, as well as alizarin red staining. Ectopic bone formation by the cells was assessed using micro‐computed tomography (μCT) and histological examination.ResultsThe immortalized cell line we established using SV40 LTA, which we termed iRBMSCs, was non‐tumorigenic and maintained long‐term proliferative activity. We further discovered that BMP9 (MOI = 30) effectively induced the osteogenic differentiation capacity of iRBMSCs in vitro, and there was a synergy with GelMA hydrogel in inducing osteogenic differentiation of the iRBMSCs in vivo.ConclusionWe confirmed that iRBMSCs are promising as a stable cell line source for bone defect repair engineering.

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Exosome therapy in female reproductive aging

Zhang,

Wang

2024

Extracellular Vesicle

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The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence

Cited by 6 publications

References 115 publications

Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling

Novel Immortalized Human Multipotent Mesenchymal Stromal Cell Line for Studying Hormonal Signaling

Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

Exosome therapy in female reproductive aging

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