The outer membrane of Proteus mirabilis I. Isolation and characterization of the outer and cytoplasmic membrane fractions

The effect of lipid a on the fluidity and permeability propertes of phospholipid dispersions

“…Cell lipids were extracted by three successive extractions with chloroform-methanol (2/ 1) at 45'C for 1 h. LPS was extracted by the phenol/chloroform/light petroleum procedure [ 141. Ribonucleic acid contaminating the LPS preparations was removed by ribo-nuclease treatment [3]. Lipid A was liberated from LPS by hydrolysis in 0.1 N acetic acid for 1 h at 100°C.…”

“…The outer membrane differs from the cytoplasmic membrane in a number of biochemical and physiological properties. Both membranes contain the same classes of lipids but only the outer membrane contains lipopolysaccharide (LPS) [2,3]. In addition, the outer membrane of smooth Gram-negative bacteria was found to be impermeable to hydrophobic components capable of penetrating through the cytoplasmic membrane [4,5].…”

Composition of the outer membrane of Proteus mirabilis in relation to serum sensitivity in progressive stages of cell form defectiveness

“…Electron micrographs of spheroplasts of P . mirabilis show extensive loss of outer membrane fragments in the form of spherical particles which slough off the surface (Hofschneider & Martin, 1968). Thin sections of penicillin-induced spheroplasts of E. cdi viewed in the electron microscope show occasional large spaces between inner and outer membranes (Schnaitman, 1970) as well as areas of total detachment of the outer membrane from the cell surface (Gumpert et al, 1971).…”

“…They represent proteins with approximate molecular masses of 39, 36, 19 and 15 kDa (Gmeiner, 1981;Nixdorff et al, 1977). The proteins in peaks 3 and 4 represent other, primarily cytoplasmic membrane proteins, which show distinctly characteristic patterns of mobility in SDS-PAGE (Hasin et al, 1974;Kroll et al, 1980;Van Heerikhuisen et al, 1975).…”

Isolation and Characterization of Outer and Cytoplasmic Membranes from Spheroplasts ofAcetobacter aceti

“…By using this technique, the successful separation of outer and cytoplasmic membranes has been achieved in other Gram-negative bacteria, such as Salmonella typhimurium,4) Pseudomonas aeruginosa, 5,6) Proteus mirabilis 7 ) and Haemophilus influenzae.…”