2020
DOI: 10.1111/hel.12731
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The outward shift of clarithromycin binding to the ribosome in mutantHelicobacter pyloristrains

Abstract: Objectives Disruption of protein synthesis, by drug‐mediated restriction of the ribosomal nascent peptide exit tunnel (NPET), may inhibit bacterial growth. Here, we have studied the secondary and tertiary structures of domain V of the 23S rRNA in the wild‐type and mutant (resistant) H. pylori strains and their mechanisms of interaction with clarithromycin (CLA). Methods H pylori strains, isolated from cultured gastric biopsies, underwent CLA susceptibility testing by E test, followed by PCR amplification and s… Show more

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Cited by 4 publications
(5 citation statements)
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“…One hundred clinical H. pylori isolates were collected from 290 dyspeptic patients, via upper endoscopy, from 2013 to 2018, at Amiralam Hospital, Tehran, Iran. Gastric biopsy specimens were cultured onto Brucella agar medium (Merck, Germany), supplemented with 10% defibrinated sheep blood, amphotericin B (8 mg/L), vancomycin (10 mg/L), and trimethoprim (5 mg/L) and incubated under microaerobic conditions (O2, 5%; CO2, 10%; N2, 85%) at 37 °C for 3–5 days [ 51 ]. Sample collection was performed according to the approved protocols by the Committee on Ethical Issues in Medical Research, Pasteur Institute of Iran (Ref.No.IR.PII.REC.1394.57) and every patient provided written informed consent.…”
Section: Methodsmentioning
confidence: 99%
“…One hundred clinical H. pylori isolates were collected from 290 dyspeptic patients, via upper endoscopy, from 2013 to 2018, at Amiralam Hospital, Tehran, Iran. Gastric biopsy specimens were cultured onto Brucella agar medium (Merck, Germany), supplemented with 10% defibrinated sheep blood, amphotericin B (8 mg/L), vancomycin (10 mg/L), and trimethoprim (5 mg/L) and incubated under microaerobic conditions (O2, 5%; CO2, 10%; N2, 85%) at 37 °C for 3–5 days [ 51 ]. Sample collection was performed according to the approved protocols by the Committee on Ethical Issues in Medical Research, Pasteur Institute of Iran (Ref.No.IR.PII.REC.1394.57) and every patient provided written informed consent.…”
Section: Methodsmentioning
confidence: 99%
“…Then we further performed subgroup analysis on antibiotics dose administration. In our study, we noticed that the actual cure rate of low-dose antibiotics treatment 32 For CLA-resistant H. pylori, frequent exposure to CLA increased the chance of gene mutation. This may increase the probability of mutations in genes encoding penicillin-binding proteins.…”
Section: Tablementioning
confidence: 73%
“…This leads to ribosome allostery, which reduced the affinity of the CLA binding site. 32 For CLA-resistant H. pylori , frequent exposure to CLA increased the chance of gene mutation. This may increase the probability of mutations in genes encoding penicillin-binding proteins.…”
Section: Discussionmentioning
confidence: 99%
“…Since we concentrated on the subtle differences among synergy, additive, and antagonism, all 91 pairwise combinations fall into three categories, according to the α-score ( Supplementary Table S1 ). Targets of these antibiotics were collected from previous literature studies ( Pongs et al, 1973 ; Shen and Pernet, 1985 ; Buck and Cooperman, 1990 ; Pan et al, 1996 ; Onodera et al, 2002 ; Aracena et al, 2014 ; Kocaoglu and Carlson, 2015 ; Wekselman et al, 2017 ; Lin et al, 2018 ; Salehi et al, 2020 ; Wróbel et al, 2020 ). Because some antibiotics are RNA-targeted small molecules, ribosomal proteins that affect antibiotic binding are considered targets of antibiotics.…”
Section: Resultsmentioning
confidence: 99%