The overexpression of an Amaranthus hypochondriacus NF-YC gene modifies growth and confers water deficit stress resistance in Arabidopsis

Palmeros‐Suárez, Paola Andrea; Massange-Sánchez, Julio A.; Martínez‐Gallardo, Norma; Montero-Vargas, Josaphat Miguel; Gómez-Leyva, Juan Florencio; Délano‐Frier, John Paul

doi:10.1016/j.plantsci.2015.08.010

Cited by 54 publications

(47 citation statements)

References 98 publications

(125 reference statements)

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“…It was previously shown that the WDS response in Ahypo roots included the accumulation of osmolytes and increased levels of ROS scavenging and heat shock proteins, together with the induction of certain TFs (Huerta-Ocampo et al , 2011). Several other amaranth genes have been subsequently proposed as possible contributing factors to increased tolerance against several (a)abiotic stresses in grain amaranth, including an orphan gene (Massange-Sánchez et al , 2015), a gene with an unknown function domain (Palmeros-Súarez et al , 2017) and various TF genes (Palmeros Súarez et al , 2015; Massange-Sánchez et al , 2016). The above genes were induced in grain amaranth by several stress conditions and frequently conferred stress tolerance when overexpressed in Arabidopsis plants.…”

Section: Discussionmentioning

confidence: 99%

“…Quantitative gene expression analysis using SYBR Green detection chemistry (Bio-Rad, Hercules, CA, USA) was performed as described previously (Palmeros-Suárez et al , 2015). Primers design for the amplification of the pertinent amaranth gene transcripts employed a published methodology (Thornton and Basu, 2011) and was based on recently published genomic data (Clouse et al , 2016) (Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…Leaf and root samples collected from control plants, and from plants subjected to MWDS, SWDS or R, were used to quantify soluble NSCs and Pro contents, according to Palmeros-Suárez et al (2015).…”

Section: Methodsmentioning

confidence: 99%

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Varying water deficit stress (WDS) tolerance in grain amaranths involves multifactorial shifts in WDS-related responses

González-Rodríguez

Cisneros-Hernández

Martínez‐Gallardo

et al. 2017

Preprint

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In this study, water deficit stress (WDS)-tolerance in several cultivars of grain amaranth species (Amaranthus hypochondriacus [Ahypo], A. cruentus [Acru] and A. caudatus [Acau]), in addition to A. hybridus (Ahyb), an ancestral amaranth, was examined. Ahypo was the most WDS-tolerant species, whereas Acau and Ahyb were WDS-sensitive. Data revealed that the differential WDS tolerance observed was multifactorial. It involved increased proline and raffinose (Raf) in leaves and/ or roots. Higher foliar Raf coincided with induced Galactinol synthase 1 (AhGolS1) and Raffinose synthase (AhRafS) expression. Unknown compounds, possibly larger RFOs, also accumulated in leaves of WDS-tolerant amaranths, which had high Raf/ Verbascose ratios. Distinct nonstructural carbohydrate (NSC) accumulation patterns were observed in tolerant species under WDS and recovery, such as: i) high Hex/ Suc ratios in roots coupled to increased cell wall and vacuolar invertase and sucrose synthase activities; ii) a severer depletion of starch reserves; iii) lower NSC content in leaves, and iv) higher basal hexose levels in roots which further increased under WDS. WDS-marker gene expression patterns proposed a link between amaranth’s WDS tolerance and abscisic acid-dependent signaling. Results obtained also suggest that AhTRE, AhTPS9, AhTPS11, AhGolS1 and AhRafS are reliable gene markers of WDS tolerance in amaranth.HighlightDifferential water deficit stress tolerance in grain amaranths and their ancestor, Amaranthus hybridus, is a multifactorial process involving various biochemical changes and modified expression patterns of key stress-related genes.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Varying water deficit stress (WDS) tolerance in grain amaranths involves multifactorial shifts in WDS-related responses

González-Rodríguez

Cisneros-Hernández

Martínez‐Gallardo

et al. 2017

Preprint

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“…The nuclear factor Y (NF-Y) TFs are emerging as important regulators of drought-stress response, particularly with respect to ABA biosynthesis. For instance, ectopic expression of Amaranthus hypochondriacus NF-YC gene (AhNF-YC) in Arabidopsis and overexpression of Bermuda grass Cdt-NF-YC1 in rice has shown that these genes confer drought tolerance [35,36]. Cdt-NF-YC1 induces expression of both ABA-responsive genes (e.g., OsRAB16A, OsLEA3, OsP5CS1 and OsLIP9) and signaling genes (e.g., OsABI2 and OsNCED3), as well as, ABA independent genes (e.g., OsDREB1A, Os-DREB2B and OsDREB1B).…”

Section: Advances In Plant Tolerance To Droughtmentioning

confidence: 99%

Advances in Plant Tolerance to Abiotic Stresses

Onaga¹,

Wydra²

2016

Plant Genomics

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During the last 50 years, it has been shown that abiotic stresses influence plant growth and crop production greatly, and crop yields have evidently stagnated or decreased in economically important crops, where only high inputs assure high yields. The recent manifesting effects of climate change are considered to have aggravated the negative effects of abiotic stresses on plant productivity. On the other hand, the complexity of plant mechanisms controlling important traits and the limited availability of germplasm for tolerance to certain stresses have restricted genetic advances in major crops for increased yields or for improved other traits. However, some level of success has been achieved in understanding crop tolerance to abiotic stresses; for instance, identification of abscisic acid (ABA) receptors (e.g., ABA-responsive element (ABRE) binding protein/ABRE binding factor (AREB/ABF) transcription factors), and other regulons (e.g., WRKYs, MYB/ MYCs, NACs, HSFs, bZIPs and nuclear factor-Y (NF-Y)), has shown potential promise to improve plant tolerance to abiotic stresses. Apart from these major regulons, studies on the post-transcriptional regulation of stress-responsive genes have provided additional opportunities for addressing the molecular basis of cellular stress responses in plants. This chapter focuses on the progress in the study of plant tolerance to abiotic stresses, and describes the major tolerance pathways and implicated signaling factors that have been identified, so far. To link basic and applied research, genes and proteins that play functional roles in mitigating abiotic stress damage are summarized and discussed.In the past 100 years, conventional breeding ( Figure 2; based on observed variation and controlled mating) approaches have randomly exploited these plant tolerance mechanisms with limited success. Moreover, in vitro induced variations have also shown little progress in the improvement of plants against abiotic stresses. These conventional breeding approaches are limited by the complexity of stress tolerance traits coupled with less genetic Plant Genomics 168 variation exhibited by most crops due to domestication bottlenecks. The recent reports that the cultivated gene pool of major cereal crops, e.g., rice, maize and wheat, has reduced in genetic variation compared to wild relatives [10-12], raises concern, and could probably undermine the current efforts to identify genetic sources of resistance within the cultivated genepools. It is important, therefore, to consider exploring alternative sources of resistance by incorporating modern techniques into traditional breeding strategies to develop stresstolerant crops (Figure 2).Recently, with the support of genomics, targeted genetic studies involving QTL mapping and validation, identification of key regulatory genes, e.g., genes encoding for ABA receptors, developments in transcriptional and post-transcriptional regulation of stress-responsive genes and studies on hormonal interactions during plant response to stress, have provided opp...

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“…Studies in recent years have shown that individual NF-Y subunits in plants are involved in many important growth processes, especially in embryogenesis [11,12] and seed maturation [13][14][15], chloroplast synthesis [16][17][18], tissue division [19] and others processes. Simultaneously, the NF-Y subunit also plays an important role in response to stress, such as drought stress [20][21][22][23][24][25]. It is worth noting that more and more studies have found that the NF-Y subunit participates in the photoperiodic regulation of flowering induction pathways, and that different subunits function differently [26][27][28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants

et al. 2019

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Background: Compared with annual herbaceous plants, woody perennials require a longer period of juvenile phase to flowering, and many traits can be only expressed in adulthood, which seriously makes the breeding efficiency of new varieties slower. For the study of poplar early flowering, the main focus is on the study Arabidopsis homologue gene CO/FT. Based on studies of Arabidopsis, rice and other plant species, some important research progress has been made on the regulation of flowering time by NF-Y subunits. However, little is known about the function of NF-Y regulating flowering in poplar. Results: In the present study, we have identified PtNF-YB family members in poplar and focus on the function of the PtNF-YB1 regulate flowering timing using transgenic Arabidopsis and tomato. To understand this mechanisms, the expression levels of three known flowering genes (CO, FT and SOC1) were examined with RT-PCR in transgenic Arabidopsis. We used the Y2H and BiFC to assay the interactions between PtNF-YB1 and PtCO (PtCO1 and PtCO2) proteins. Finally, the potential molecular mechanism model in which PtNF-YB1 play a role in regulating flowering in poplar was discussed. Conclusions: In this study, we have characterized the poplar NF-YB gene family and confirmed the function of the PtNF-YB1 regulate flowering timing. At the same time, we found that the function of PtNF-YB1 to improve early flowering can overcome species barriers. Therefore, PtNF-YB1 can be used as a potential candidate gene to improve early flowering by genetic transformation in poplar and other crops.

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The overexpression of an Amaranthus hypochondriacus NF-YC gene modifies growth and confers water deficit stress resistance in Arabidopsis

Cited by 54 publications

References 98 publications

Varying water deficit stress (WDS) tolerance in grain amaranths involves multifactorial shifts in WDS-related responses

Varying water deficit stress (WDS) tolerance in grain amaranths involves multifactorial shifts in WDS-related responses

Advances in Plant Tolerance to Abiotic Stresses

Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants

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