The overexpression of NCED results in waterlogging sensitivity in soybean

Oliveira, Fabiane Kletke de; da-Silva, Cristiane Jovelina; Garcia, Natália; Agualongo, Darwin Alexis Pomagualli; Oliveira, A.; Kanamori, Norihito; Takasaki, Hironori; Urano, Kaoru; Shinozaki, Kazuo; Nakashima, Kenichiro; Yamaguchi‐Shinozaki, Kazuko; Nepomuceno, Alexandre Lima; Mertz-Henning, L. M.; Amarante, Luciano do

doi:10.1016/j.stress.2021.100047

Cited by 12 publications

(5 citation statements)

References 76 publications

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“…These outcomes specified that the introduced GmAdh2 gene might have brought some change in glycolysis and alcohol fermentation and enhanced the germination of transgenic soybeans under flooding stress ( Tougou et al., 2012 ). Recently, De Oliveira et al. (2022a) evaluated the transgenic soybean line overexpressing the NCED gene (2Ha11).…”

Section: Transgenic Breeding For Flood Tolerance In Soybeanmentioning

confidence: 99%

“…NCED overexpression reduced seed weight and grain yield in the 2Ha11 line under waterlogging at the reproductive phase. These outcomes suggested that the overexpression of NCED activates an enhanced flooding sensitivity in soybean plants, particularly after treatment with waterlogging at the reproductive phase ( De Oliveira et al., 2022a ). Genetic engineered soybean could play a crucial part in the advancement of flood-tolerant soybean cultivars.…”

Section: Transgenic Breeding For Flood Tolerance In Soybeanmentioning

confidence: 99%

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The intervention of classical and molecular breeding approaches to enhance flooding stress tolerance in soybean – An review

et al. 2022

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Abiotic stresses and climate changes cause severe loss of yield and quality of crops and reduce the production area worldwide. Flooding stress curtails soybean growth, yield, and quality and ultimately threatens the global food supply chain. Flooding tolerance is a multigenic trait. Tremendous research in molecular breeding explored the potential genomic regions governing flood tolerance in soybean. The most robust way to develop flooding tolerance in soybean is by using molecular methods, including quantitative trait loci (QTL) mapping, identification of transcriptomes, transcription factor analysis, CRISPR/Cas9, and to some extent, genome-wide association studies (GWAS), and multi-omics techniques. These powerful molecular tools have deepened our knowledge about the molecular mechanism of flooding stress tolerance. Besides all this, using conventional breeding methods (hybridization, introduction, and backcrossing) and other agronomic practices is also helpful in combating the rising flooding threats to the soybean crop. The current review aims to summarize recent advancements in breeding flood-tolerant soybean, mainly by using molecular and conventional tools and their prospects. This updated picture will be a treasure trove for future researchers to comprehend the foundation of flooding tolerance in soybean and cover the given research gaps to develop tolerant soybean cultivars able to sustain growth under extreme climatic changes.

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Section: Transgenic Breeding For Flood Tolerance In Soybeanmentioning

confidence: 99%

Section: Transgenic Breeding For Flood Tolerance In Soybeanmentioning

confidence: 99%

The intervention of classical and molecular breeding approaches to enhance flooding stress tolerance in soybean – An review

et al. 2022

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“…It was shown recently, that overexpression of AtNCED3 in G. max increased oxidative stress, decreased fermentation in plants exposed to waterlogging, and, ultimately, reduced seed weight and yield [ 49 ]. We suggested that, while GmNCED1 provides an increase in ABA level after stress treatment for stress acclimation, GmNCED5 probably maintains a basic level of ABA for successful growth and development in normal conditions.…”

Section: Discussionmentioning

confidence: 99%

ABA-Dependent Regulation of Calcium-Dependent Protein Kinase Gene GmCDPK5 in Cultivated and Wild Soybeans

Veremeichik

Brodovskaya

Grigorchuk

et al. 2022

Life

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Calcium-dependent protein kinases (CDPKs) regulate plant development and stress responses. However, the interaction of these protein kinases with the abscisic acid (ABA) stress hormone signalling system has not been studied in detail. In Arabidopsis, AtCPK1 plays an important role in the acclimation of plants to environmental stresses. Phylogenetic and molecular analyses showed that, among 50 isoforms of Glycine max (L.) Merrill CDPKs, the GmCDPK27/GmCDPK48, GmCDPK5/GmCDPK24, and GmCDPK10/GmCDPK46 paralogous pairs were the isoforms most related to AtCDPK1. We investigated the expression of the corresponding six GmCDPKs genes during treatment with cold, heat, and salt stress. Wild soybean was the most resistant to stresses, and among the three cultivars studied (Sfera, Hodgson, and Hefeng25), Sfera was close to the wild type in terms of resistance. GmCDPK5 and GmCDPK10 were the most responsive to stress treatments, especially in wild soybean, compared with cultivars. Among the studied GmCDPK isoforms, only GmCDPK5 expression increased after treatment with abscisic acid (ABA) in a dose- and time-dependent manner. Targeted LC-MS/MS analysis of endogenous ABA levels showed that wild soybean and Sfera had nearly twice the ABA content of Hodgson and Hefeng25. An analysis of the expression of marker genes involved in ABA biosynthesis showed that GmNCED1-gene-encoding 9-cis-epoxycarotenoid dioxygenase 1 is induced to the greatest extent in wild soybean and Sfera under salt, cold, and heat exposure. Our data established a correlation between the induction of GmCDPK5 and ABA biosynthesis genes. GmCDPK5 is an interesting target for genetic and bioengineering purposes and can be used for genetic editing, overexpression, or as a marker gene in soybean varieties growing under unfavourable conditions.

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“…This slow rate of evolution might play a role in the functional divergence of NCED subfamilies across different tissues. For example, NCED subfamilies may have different expression patterns and activity levels in different plant tissues, which could contribute to regulating ABA biosynthesis and plant responses to environmental cues (Nakashima et al, 2022). In investigating ABA biosynthesis and its function in plant growth and development, identifying the NCED gene is a signi cant turning point.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and In-silico Expression Analysis of CCO Gene Family in Sunflower (Helianthus annnus)

Sami,

Haider,

Shafiq

et al. 2023

Preprint

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Carotenoid cleavage oxygenases (CCOs) enzymes play an important role in plant growth and development by producing a wide array of apocarotenoids and their derivatives. These compounds are vital for colouring flowers and fruits and synthesizing plant hormones such as abscisic acid and strigolactones. Despite their importance, the gene family responsible for CCOs in sunflowers has not been studied. In this study, we identify the CCO genes of the sunflower plant to fill this knowledge gap. Phylogenetic and synteny analysis indicated that the Helianthus annnus CCO (HaCCO) proteins are conserved in different plant species and they can be divided into three subgroups based on their conserved domains. MEME analysis and multiple sequence alignment showed that conserved motifs exist in the sequence of HaCCO genes. Cis-regulatory elements (CREs) analysis of the HaCCO genes revealed the presence of multiple plant hormones and abiotic and biotic responsive elements in their promoters, suggesting that these genes might be responsive to plant hormones, developmental and drought stress, which could be utilized to develop more resilient crops. Genes belonging to the 9-cis-epoxy carotenoid dioxygenases (NCED) subgroups predominantly exhibit chloroplast localization, whereas the genes found in other groups are primarily localized in the cytoplasm. These 21 identified HaCCOs were regulated by 60 miRNAs indicating the crucial role of microRNAs in gene regulation in sunflowers. Gene expression analysis under drought stress revealed significant up-regulation of HaNCED16 and HaNCED19 genes that provide pivotal role in ABA hormone biosynthesis. While during organ-specific gene expression analysis HaCCD12 and HaCCD20 genes exhibit higher activity in leaves, indicating a potential role in leaf pigmentation. These studies provide a foundation for future research on the regulation and functions of this gene family. It could potentially be utilized to develop molecular markers that could be used in breeding programs to develop new sunflower lines.

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The overexpression of NCED results in waterlogging sensitivity in soybean

Cited by 12 publications

References 76 publications

The intervention of classical and molecular breeding approaches to enhance flooding stress tolerance in soybean – An review

The intervention of classical and molecular breeding approaches to enhance flooding stress tolerance in soybean – An review

ABA-Dependent Regulation of Calcium-Dependent Protein Kinase Gene GmCDPK5 in Cultivated and Wild Soybeans

Genome-Wide Identification and In-silico Expression Analysis of CCO Gene Family in Sunflower (Helianthus annnus)

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