Background: A high prevalence of adolescent dating violence (ADV) has been documented in recent years. However, the majority of ADV studies have been conducted in North America and moreover, ADV studies have primarily focused on high school or college populations. Objective: To investigate victimization and perpetration of ADV and related gender differences in a sample of Danish seventh-grade students. Method: In total, 2934 seventh-grade students (M = 3.5, SD = 0.5) filled out questionnaires at school. Results: The prevalence of victimization of emotional, physical and sexual ADV was 32.2%, 11.2% and 10.6%, and the prevalence of perpetration of emotional, physical and sexual ADV was 20.6%, 6% and 2.1%. One out of five students reported both victimization and perpetration of one of the three ADV types and 14.3% and 6% reported multiple forms of ADV victimization and perpetration respectively. Moreover, gender differences in the prevalence of ADV and the co-occurrence of ADV victimization and perpetration were identified. Conclusion: The present study highlights that a large proportion of Danish seventh-grade students are experiencing ADV, and that ADV preventive programs are relevant already in early adolescence. Based on the high proportion of students reporting co-occurrence of victimization and perpetration experiences, comprehensive preventive programs that focus on both victimization and perpetration experiences and the transmission of violence are recommended.