The Oviduct: Functional Genomic and Proteomic Approach

Mondéjar, Irene; Acuña, OS; Izquierdo-Rico, M. J.; Coy, Pilar; Sánchez, Manuel A. Bueno

doi:10.1111/j.1439-0531.2012.02027.x

Cited by 46 publications

(44 citation statements)

References 69 publications

(160 reference statements)

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“…Thus, fucosidase activity, present in the oviductal fluid (Carrasco et al 2008b) could contribute to the regulation of the binding. Additionally, the presence of annexin in the oviductal fluid (Mondéjar et al 2012a) could also participate in such regulation, since the atypical secretion of this protein has been reported previously (Christmas et al 1991). …”

Section: The Spermatozoon In the Oviductmentioning

confidence: 82%

“…Due to the complex protein composition found in the oviductal fluid (Avilés et al 2010, Mondéjar et al 2012a), two additional mechanisms could contribute to the regulation of the sperm oviductal interaction. First, activities for different glycosidases have been detected in the oviductal fluid showing variations along the estrous cycle (Carrasco et al 2008a, Carrasco et al 2008b).…”

Section: The Spermatozoon In the Oviductmentioning

confidence: 99%

“…It seems clear that gametes modulate their own microenvironment and it can be anticipated that in the immediate future new molecules of oviductal origin participating in the fertilization process will be identified. Data from the whole oviductal transcriptome in different animal models and at the different phases of the estrous cycle would be very useful to complete the puzzle of the molecular pathways playing a role in the beginning of a new life (Mondéjar et al 2012a). …”

Section: Oocyte-sperm-oviduct Interactionsmentioning

confidence: 99%

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Roles of the oviduct in mammalian fertilization

et al. 2012

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The oviduct or Fallopian tube is the anatomical region where every new life begins in mammalian species. After a long journey, the spermatozoa meet the oocyte in the specific site of the oviduct named ampulla, and fertilization takes place. The successful fertilization depends on several biological processes which occur in the oviduct some hours before this rendezvous and affect both gametes. Estrogen and progesterone, released from the ovary, orchestrate a series of changes by genomic- and non-genomic pathways in the oviductal epithelium affecting gene expression, proteome and secretion of its cells into the fluid bathing the oviductal lumen. In addition, new regulatory molecules are being discovered playing important roles in oviductal physiology and fertilization. The present review tries to describe these processes, building a comprehensive map of the physiology of the oviduct, to better understand the importance of this organ in reproduction. With this purpose, gamete transport, sperm and oocyte changes in the oviductal environment and other interactions between gametes and oviduct are discussed in light of recent publications in the field.

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Section: The Spermatozoon In the Oviductmentioning

confidence: 82%

Section: The Spermatozoon In the Oviductmentioning

confidence: 99%

Section: Oocyte-sperm-oviduct Interactionsmentioning

confidence: 99%

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Roles of the oviduct in mammalian fertilization

et al. 2012

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“…The oviduct is an import part of the genital tract as it is the place for fertilization and fosters embryo during embryogenesis [27] . As reported by two previous studies [10,24] , the number of mast cells increases in the bovine oviduct mucosa especially in the isthmus during the luteal phase of the cycle.…”

Section: Discussionmentioning

confidence: 99%

Östral ve Luteal Dönemlerdeki Koyunların Oviduktunda Mast Hücrelerinin İncelenmesi

Kürüm¹,

Özen²,

Karahan³

et al. 2014

Kafkas Univ Vet Fak Derg

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Mast cells are heterogeneous cell populations that play significant roles in many organs and systems and involve various physiological processes. We aimed to evaluate mast cells in the ovine oviduct mucosa by means of their staining and ultrastructural characteristics. The ovine oviduct samples of Akkaraman breed were collected from the slaughterhouse and they are categorized as luteal and oestral phases. They were fixed either with 10% formalin or IFAA and stained with Toluidine blue and Alcian blue and Safranin O (Ab/SO). Mast cells were located near blood vessels and basal membrane. Compared to 10% formalin fixed tissues, the number of mast cells were higher in IFAA fixed tissues (P=0.003). Importantly all mast cells Ab(+) and SO(-) so that they were categorized as mucosal type. The number of mast cells did not differ between luteal and oestral phases (P>0.05). However, there were significant differences among different regions of the oviduct with a less count in the isthmus regions (P=0.006). Transmission electron microscopy revealed that the oviduct mast cells contained two types of granules: an electron lucent, electron dense. Some electron lucent granules contained an eccentrically located crystal-like structure. The significance of less mast cell counts in the isthmus and the eccentrically located single crystal-like structure should be further investigated in future studies. Keywords: Mast cell, Oviduct, Ovine, Sexual cycle, Electron microscopy Östral ve Luteal Dönemlerdeki Koyunların Oviduktunda Mast Hücrelerinin İncelenmesi ÖzetMast hücreleri birçok organ ve sistemdeki çeşitli fizyolojik süreçlerde önemli rolleri bulunan heterojen hücre topluluğudur. Bu çalışmada koyun ovidukt mukozasındaki mast hücrelerini boyanma özellikleri ve ince yapı düzeyinde incelemeyi amaçladık. Mezbahanede kesim sırasında östral ve luteal dönemleri Akkaraman koyunlarının oviduct örnekleri alındı. Alınan örnekler %10 formol ve IFAA ile tespit edilerek Toluidin blue ve Alcian blue-Safranin O (Ab/SO) ile boyandı. Mast hücrelerinin, kan damarlarının ve ovidukt epitelinin bazal membranına yakın olarak yerleştiği görüldü. IFAA ile tespit edilen dokulardaki mast hücre sayısının %10 formolle tespit edilen dokulara göre daha fazla olduğu belirlendi (P=0.003). Dikkat çekici olarak mast hücrelerinin tamamının Ab(+) ve SO(-) olduğu görüldü. Luteal ve östral dönemler arasında mast hücre sayısı yönünden fark bulunamadı (P>0.05). Fakat ovidukt bölgeleri arasında istatistiksel olarak önemli fark vardı ve mast hücre sayısı istmusta daha azdı (P=0.006). Elektron mikroskobik incelemelerde mast hücrelerinin elektron açık ve elektron koyu olmak üzere iki tip granül içerdiği ortaya konuldu. Bazı elektron açık granüllerde eksantrik yerleşimli elektron koyu kristal benzeri bir yapının varlığı gözlendi. İstmusta mast hücrelerinin daha az sayıda görülmesinin ve granüllerdeki eksantrik yerleşimli kristal benzeri yapının daha sonraki çalışmalarda araştırılması önemlidir.

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“…Gene expression changes in the pig oviduct have been reported after inseminating with X-or Y-bearing spermatozoa, which suggests that not only the entry of spermatozoa but also their chromosomal sex seems relevant, despite we do not know the mechanisms behind (Almiñana et al, 2014). Gene expression changes have also been reported in the internal genital tract of other species (Fazeli et al, 2004;Kodithuwakku et al 2007;Mondéjar et al, 2012). Although the composition of the SF/SP proteome of boars and chickens might be relevant to disclose eventual signal molecules placed by the male at mating, this area has so far been only partially studied.…”

Section: Entry Of Semen Induces Gene Expression Changes In Female Genmentioning

confidence: 99%

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Atikuzzaman¹

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Internal fertilization occurs in birds and eutherian mammals. Foetal development, however, is either extra-respectively intra-corpore (egg vs uterus). In these animal classes, the female genital tract stores ejaculated spermatozoa into a restricted oviductal segment; the functional pre-ovulatory sperm reservoir, where they survive until ovulation/s occur. Paradoxically, this immunologically foreign sperm suspension in seminal fluid/plasma, often microbiologically contaminated, ought to be promptly eliminated by the female local immune defence which, instead, tolerates its presence. The female immune tolerance is presumably signalled via a biochemical interplay of spermatozoa, as well as the peptides and proteins of the extracellular seminal fluid, with female epithelial and immune cells. Such interplay can result in gene expression shifts in the sperm reservoir in relation to variations in fertility. To further aid our understanding of the underlying mechanisms, this thesis studied the proteome of the seminal fluid (using 2D SDS-PAGE and mass spectrometry) including cytokine content (using Luminex and/or ELISA) of healthy, sexually mature and fertile boars and cocks. As well, gene expression changes (using cDNA microarray) in the oviductal sperm reservoirs of sexuallymature females, mated or artificially infused with homologous sperm-free seminal fluid/plasma were studied. Pigs were of commercial, fertility-selected modern breeds (Landrace), while chicken belonged to the ancestor Red Junglefowl (RJF, low egg laying-capacity), a selected egg-layer White Leghorn (WL) and of their Advanced Intercross Line (AIL). Ejaculates were manually collected as single sample in cocks or as the sperm-rich fraction [SRF] and the post-SRF fraction in boars to harvest seminal fluid/plasma for proteome/cytokine and infusionstudies. Oviducts were retrieved for gene-expression analyses via microarray immediately post-mortem (chicken) or at surgery (pig), 24 h after mating or genital infusion. In pigs, the protein-rich seminal plasma showed the highest amounts of cytokines [interferon-γ, interferon gamma-induced protein 10 (IP-10/CXCL10), macrophage derived chemokine (MDC/CCL22), growth-regulated oncogene (GRO/CXCL1), granulocyte-macrophage colony-stimulating factor (GM-CSF), monocyte chemo-attractant protein-1 (MCP-1/ CCL2), interleukin (IL)-6, IL-8/CXCL8, IL-10, IL-15, IL-17 and transforming growth factor (TGF)-β1-3) in the larger, protein-rich and sperm-poor post-SRF, indicating its main immune signalling influence. Chicken showed also a plethora of seminal fluid proteins with serum albumin and ovotransferrin being conserved through selection/evolution. However, they showed fewer cytokines than pigs, as the anti-inflammatory/immune-modulatory TGF-β2 or the proinflammatory CXCL10. The RJF contained fewer immune system process proteins and lacked TGF-β2 compared to WL and AIL, suggesting selection for increased fertility could be associated with higher expression of immune-regulating peptides/proteins. The oviductal sperm re...

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The Oviduct: Functional Genomic and Proteomic Approach

Cited by 46 publications

References 69 publications

Roles of the oviduct in mammalian fertilization

Roles of the oviduct in mammalian fertilization

Östral ve Luteal Dönemlerdeki Koyunların Oviduktunda Mast Hücrelerinin İncelenmesi

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

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