The oxidation of aqueous ferrous sulphate by thiobacillus ferrooxidans

MacDonald, Drew; Clark, Robert H.

doi:10.1002/cjce.5450480604

Cited by 78 publications

(25 citation statements)

References 21 publications

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“…No prominent adsorption of bacterial cells was observed on the eroded surfaces of the pyrite even though the dissolution of iron from pyrite and iron oxidation were markedly enhanced in the presence of a great number of T, ferrooxidans (109-1010 cells/ml). T. ferrooxidans apparently attaches to solid surfaces such as sulfur, sulfide minerals, aluminium, glass wall of culture flask, and hydrated ferric precipitates (4)(5)(6)(7)(8)(9). However, with a few exceptions (S, 7-9), quantitative information has not been available on the bacterial attachment to solid surfaces.…”

Section: Bacterial Growth and Iron Dissolution In M9k Pyrite Mediummentioning

confidence: 99%

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Wakao

Mishina

Sakurai

et al. 1984

J. Gen. Appl. Microbiol.

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The cells of 'Thiobacillus ferrooxidans were rapidly adsorbed on the solid surfaces of an agitated flask containing 1 % pulp density of pyrite particles. More than ca. 99 % of the inoculated cells were adsorbed. However, considerably fewer cells were adsorbed on pyrite particles than on the glass wall of the flask. Scanning electron microscope observation revealed that T. ferrooxidans cells were adsorbed aggregatively on restricted areas of the pyrite particles. The surfaces of the pyrite particles were characteristically eroded to show etched polyhedral pits, but without prominent cell adsorption of the extensively eroded surfaces during markedly enhanced leaching. When T. ferrooxidans cells were adsorbed on the solid surfaces, the iron-oxidizing activity of the bacteria was strongly inhibited, resulting in a failure to enhance pyrite oxidation. Adsorbed cells did not proliferate on the solid surfaces. When the adsorbed cells were released into an aqueous phase by the addition of the surface active agent Tween 20, the bacterial iron-oxidizing activity inhibited by adsorption was recovered and, as a result, pyrite oxidation was markedly promoted. Significant enhancement of pyrite oxidation by T, ferrooxidans was ascribed to the development and iron-oxidizing activity of the freely dispersed cells in an aqueous phase. The function of the surface active agent is to prevent tenacious adsorption of the bacterial cells to solid surfaces and organic substances examined, such as protein, nucleic acid, yeast extract, peptone, and cell free extracts, operate in the same way as the surface active agent. The enhancement of the bacterial pyrite oxidation by the intact cells of Thiobacillus thiooxidans is thought to be attributable to the organic substances excreted from T. thiooxidans cells and/or to the exchange adsorption of cells of both thiobacilli. The present results indicate that bacterial concentration 63

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Section: Bacterial Growth and Iron Dissolution In M9k Pyrite Mediummentioning

confidence: 99%

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Wakao

Mishina

Sakurai

et al. 1984

J. Gen. Appl. Microbiol.

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“…To improve Fe 2+ oxidation rate, many efforts have been made to maintain a high density of biomass through the immobilization of A. ferrooxidans onto solid matrices (Grishin and Tuovinen, 1988;Umita, 1996). The natural tendency of A. ferrooxidans to grow on surfaces makes it an ideal organism for cell immobilization (MacDonald and Clark, 1970;Nemati et al, 1998). Various immobilization matrices (glass beads, activated carbon, ion-exchange resin, polystyrene, polyurethane foam, etc.)…”

Section: Introductionmentioning

confidence: 99%

Simultaneous oxidation and precipitation of iron using jarosite immobilized Acidithiobacillus ferrooxidans and its relevance to acid mine drainage

Wang

Zhou

2012

Hydrometallurgy

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“…8) In the literature, the optimum temperature of Acidithiobacillus ferrooxidans is reported to be 306 K at pH 2.5 and 303 K at pH 1.5. 9) In this paper, the effect of physico-chemical parameters on bacterial iron oxidation rate is reported. The bacteria were isolated from effluent pond water of Dalsung Tungsten and Copper Mines, South Korea.…”

Section: )mentioning

confidence: 99%

Effect of pH and Temperature on Iron Oxidation by Mesophilic Mixed Iron Oxidizing Microflora

et al. 2008

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The aim of the work described in this paper was to establish the effect of physical parameters such as pH and temperature on the microbial growth and iron oxidation rate of an isolated iron oxidizing bacterial strain from mine effluent. The bacterial lag phase was reduced to zero by repeated subculturing in fresh nutrient media. The rate of iron oxidation was considered as the growth rate of the microorganism in its logarithmic phase. Variation of pH showed an inadequate effect within the range from 1.5 to 2.5 ensuing growth flexibility in acidic medium. With variation of temperature from 293 to 313 K, the rate increased up to 303 K and gradually decreased beyond this temperature. At the optimized values of pH 2.0 and temperature 303 K, the iron oxidation rate was 2:17 Â 10 À4 kgÁm À3 Ás À1 .

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The oxidation of aqueous ferrous sulphate by thiobacillus ferrooxidans

Cited by 78 publications

References 21 publications

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Bacterial pyrite oxidation III. Adsorption of Thiobacillus ferrooxidans cells on solid surfaces and its effect on iron release from pyrite.

Simultaneous oxidation and precipitation of iron using jarosite immobilized Acidithiobacillus ferrooxidans and its relevance to acid mine drainage

Effect of pH and Temperature on Iron Oxidation by Mesophilic Mixed Iron Oxidizing Microflora

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