The oxidation of carbon monoxide by methane-oxidizing bacteria

Hubley, John H.; Mitton, John R.; Wilkinson, John F.

doi:10.1007/bf02451778

Cited by 32 publications

(10 citation statements)

References 8 publications

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“…There are a few examples of cometabolism, as redefined above, by methaneoxidizing bacteria in the literature, e.g. ethane, propane, n-butane [8] and carbon monoxide, [9] by Methylomonas methanica, and ethane [2] and carbon monoxide [10] by Methylomonas agile. In comparison many more examples of the fortuitous oxidation of non-growth substrates by methane-oxidizing bacteria have been reported e.g.…”

Section: Resultsmentioning

confidence: 99%

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The fortuitous oxidation and cometabolism of various carbon compounds by whole-cell suspensions of Methylococcus capsulatus (Bath)

Stirling

1979

FEMS Microbiology Letters

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Section: Resultsmentioning

confidence: 99%

“…methanica [11] andM. capsulatus (Bath) [12], ethane [13] and carbon monoxide [9,10] by Methylosinus trichosporium OB3B, ethanol and formate by M. capsulatus (Texas) [14] and dimethyl ether by various unidentified methaneoxidizing bacteria [15].…”

Section: Resultsmentioning

confidence: 99%

The fortuitous oxidation and cometabolism of various carbon compounds by whole-cell suspensions of Methylococcus capsulatus (Bath)

Stirling

1979

FEMS Microbiology Letters

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“…C0 oxidation to C02 has been reported for a variety of different bacteria, both anaerobic and aerobic, including sulfate-reducing bacteria (25, 41, 42), clostridia (12,32,34), photosynthetic bacteria (13,38), methylotrophic bacteria (7,14), and H2-oxidizing bacteria (17,18,23,24,27,28,43). A hydrogen-oxidizing bacterium (28) and a photosynthetic bacterium (38) have been reported to grow fairly well on C0.…”

Section: Discussionmentioning

confidence: 99%

Carbon Monoxide Oxidation by Methanogenic Bacteria

et al. 1977

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Different species of methanogenic bacteria growing on C02 and H2 were shown to remove CO added to the gas phase. Rates up to 0.2 ,umol of CO depleted/min per 10 ml of culture containing approximately 7 mg of cells (wet weight) were observed. Methanobacterium thermoautotrophicum was selected for further study based on its ability to grow rapidly on a completely mineral medium. This species used CO as the sole energy source by disproportionating CO to C02 dnd CH4 according to the following equation: 4CO + 2H20-. 1CH4 + 3CO2. However, growth was slight, and the growth rate on CO was only 1% of that observed on H2/CO2. Growth only occurred with CO concentrations in the gas phase of lower than 50%. Growth on CO agrees with the finding that cell-free extracts of M. thermoautotrophicum contained both an acitve factor 420 (F420)dependent hydrogenase (7.7 ,umol/min per mg of protein at 350C) and a COdehydrogenating enzyme (0.2 ,tmol/min per mg of protein at 350C) that catalyzed the reduction of F,20 with CO. The properties of the CO-dehydrogenating enzyme are described. In addition to F,,0, viologen dyes were effective electron acceptors for the enzyme. The apparent Km for CO was higher than 1 mM. The reaction rate increased with increasing pH and displayed an inflection point at pH 6.7. The temperature dependence of the reaction rate followed the Arrhenius equation with an activation energy (AHt) of 14.1 kcal/mol (59.0 kJ/mol). The CO dehydrogenase activity was reversibly inactivated by low concentrations of cyanide (2 AM) and was very sensitive to inactivation by oxygen. Carbon monoxide dehydrogenase of M. thermoautotrophicum exhibited several characteristic properties found for the enzyme of Clostridium pasteurianum but differed mainly in that the clostridial enzyme did not utilize F420 as the electron acceptor.

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“…Methanotrophs are among the microorganisms capable of oxidizing CO to carbon dioxide (2,17,29). CO oxidation has been demonstrated in whole-cell suspensions and cell extracts of several strains, including M. trichosporium OB3b (26), Methylomonas albus BG8 (26), Methylomonas methanica (10,11), and Methylococcus capsulatus (Bath) (43)(44)(45). Ferenci first proposed that CO was oxidized by MMO (10), and this was subsequently confirmed by studies with purified MMO from M. trichosporium OB3b (48), M. capsulatus (Bath) (6), and Methylobacterium sp.…”

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confidence: 99%

Inhibition of trichloroethylene oxidation by the transformation intermediate carbon monoxide

Henry

Grbić-Galić

1991

Appl Environ Microbiol

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Inhibition of trichloroethylene (TCE) oxidation by the transformation intermediate carbon monoxide (CO) was evaluated with the aquifer methanotroph Methylomonas sp. strain MM2. CO was a TCE transformation intermediate. During TCE oxidation, approximately 9 mol% of the TCE was transformed to CO. CO was oxidized by Methylomonas sp. strain MM2, and when formate was provided as an electron donor, the CO oxidation rate doubled. The rate of CO oxidation without formate was 4.6 liter mg (dry weight)-1 day-1, and the rate with formate was 10.2 liter mg (dry weight)-1 day-1. CO inhibited TCE oxidation, both by exerting a demand for reductant and through competitive inhibition. The Ki for CO inhibition of TCE oxidation, 4.2 microM, was much less than the Ki for methane inhibition of TCE oxidation, 116 microM. CO also inhibited methane oxidation, and the degree of inhibition increased with increasing CO concentration. When CO was present, formate amendment was necessary for methane oxidation to occur and both substrates were simultaneously oxidized. CO at a concentration greater than that used in the inhibition studies was not toxic to Methylomonas sp. strain MM2.

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The oxidation of carbon monoxide by methane-oxidizing bacteria

Cited by 32 publications

References 8 publications

The fortuitous oxidation and cometabolism of various carbon compounds by whole-cell suspensions of Methylococcus capsulatus (Bath)

The fortuitous oxidation and cometabolism of various carbon compounds by whole-cell suspensions of Methylococcus capsulatus (Bath)

Carbon Monoxide Oxidation by Methanogenic Bacteria

Inhibition of trichloroethylene oxidation by the transformation intermediate carbon monoxide

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