The Oxidation of Glutamic Acid by Brucella Abortus

Marr, Allen G.; Olsen, Conner; Unger, H; Wilson, J. B.

doi:10.1128/jb.66.5.606-610.1953

Cited by 16 publications

(6 citation statements)

References 12 publications

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“…The rate of oxygen uptake was depressed 25 per cent. These results are similar to those reported by Marr et al (1953) on the oxidation of glutamate by Brucella abortus. No evidence for transamination was obtained in that no ninhydrin-positive spots (other than that of glutamate) were observed on paper chromatograms performed on reaction mixtures after varying time periods of incubation.…”

Section: Resultssupporting

confidence: 92%

Studies on the Metabolism of Shigella,

1958

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Section: Resultssupporting

confidence: 92%

Studies on the Metabolism of Shigella,

1958

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“…Those with strain 2308 ranged from 40 to 70, with a mean of 55. In only one instance was the Qo2(N) obtained with strain 2308 lower than that of strain 11. It has been proposed that, in strain 11, glutamate is oxidized via a-ketoglutarate through the tricarboxylic acid cycle (Marr et al, 1953). In-hibitors of some of the reactions in this sequence were used, and their effects on glutamate oxidation by cell-free extracts are given in Table 2 (van Eys et al., 1958;Sanadi, Langley, and White, 1959; Quastel and Whetman, 1924;Adelstein and Vallee, 1958).…”

Section: Resultsmentioning

confidence: 99%

GLUTAMATE METABOLISM IN BRUCELLA ABORTUS STRAINS OF LOW AND HIGH VIRULENCE

Dasinger

Wilson

1962

J Bacteriol

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Brucella abortus strains of low virulence oxidize glutamate at a high rate, whereas strains of high virulence oxidize glutamate at a relatively low rate. Results indicated that this observation was not related to differences in pathway of glutamate oxidation or to differences in total enzyme activity. Permeability studies showed that the maximal rates of glutamate accumulation were 2.8 Amoles per 2 min per g (wet wt) for a strain of high virulence and 6.2 ,moles per 2 min per g for a strain of low virulence, but equal intracellular steady-state concentrations were attained by both types of strains. Evidence

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“…Aspartase (EC 4.3.1.1) can also catalyze the de novo synthesis of a-amino groups, but this enzyme is generally repressed during cultivation in minimal media indicating that it is primarily associated with the catabolism of aspartate (13,34). Glutamate dehydrogenase can also function as a catabolic enzyme by catalyzing the oxidative deamination of glutamate, a reaction which serves as an important source of energy for a number of obligate and facultative intracellular bacterial parasites (2,5,19,21,22,29,31).…”

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confidence: 99%

Mutations Influencing the Assimilation of Nitrogen by Yersinia pestis

Brubaker

Sulen

1971

Infect Immun

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Cells of 20 isolates of Yersinia (Pasteurella) pestis exhibited an unusual nutritional requirement which could be fulfilled by glycine or L-threonine. Meiotrophic mutants which required neither of these amino acids (Gly/Thr+) were isolated from cultures of all 20 strains at a frequency of 10-7. Wild-type and Gly/Thr+ cells of 14 strains failed to utilize L-amino acids or urea (0.01 M) as primary sources of nitrogen and grew slowly in the presence of low concentrations of NH4+ (< 5 mM). Cells of six strains (termed N+) utilized certain L-amino acids and urea (0.01 M) as primary sources of nitrogen and grew rapidly in the presence of 5 mM NH4+. N+ but not Norganisms cultivated with NH4+ (0.01 M) as a primary source of nitrogen excreted a complete spectrum of naturally occurring amino acids; under this condition of growth the aspartase and particulate nicotinamide adenine dinucleotide phosphate transhydrogenase activities of N+ and N-cells were repressed. N+ meiotrophs arose at a frequency of 10t-in cultures of all 14 Nisolates, and urease-positive meiotrophs could be selected at a frequency of 10-7 from N+ but not N-cells of all 20 strains on a medium containing urea (0.01 M) as a primary source of nitrogen. These findings illustrate a reversible loss of genetic potential which has occurred during the evolution of Y. pestis as an obligate parasite and suggest that this organism is unable to efficiently remove dispensable deoxyribonucleic acid from its chromosome.

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The Oxidation of Glutamic Acid by Brucella Abortus

Cited by 16 publications

References 12 publications

Studies on the Metabolism of Shigella,

Studies on the Metabolism of Shigella,

GLUTAMATE METABOLISM IN BRUCELLA ABORTUS STRAINS OF LOW AND HIGH VIRULENCE

Mutations Influencing the Assimilation of Nitrogen by Yersinia pestis

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