The oxygen-binding properties of hemocyanin from the mollusk Concholepas concholepas

Gonzalez, Andrea F.; Nova, Esteban; Campo, Miguel del; Manubens, Augusto; Ioannes, Alfredo De; Ferreira, Jorge; Becker, María

doi:10.1016/j.bbapap.2017.08.017

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…This hypothesis is developed from consideration of the strong temperature dependence of the oxygen affinity of hemocyanins (Brix et al 1989 ; Burnett et al 1988 ; Mangum 1990 ). Various adaptations of this oxygen transport protein to different environmental conditions have been reported (González et al 2017 ; Melzner et al 2007 ; Oellermann et al 2015a , b ; Strobel et al 2012 ; Yesilyurt et al 2008 ; Zielinski et al 2001 ). These adaptations appear necessary to ensure a sufficient supply of oxygen and therefore are fundamental for molluscs to survive.…”

Section: Discussionmentioning

confidence: 99%

The Evolution of Hemocyanin Genes in Caenogastropoda: Gene Duplications and Intron Accumulation in Highly Diverse Gastropods

et al. 2021

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Hemocyanin is the oxygen transport protein of most molluscs and represents an important physiological factor that has to be well-adapted to their environments because of the strong influences of abiotic factors on its oxygen affinity. Multiple independent gene duplications and intron gains have been reported for hemocyanin genes of Tectipleura (Heterobranchia) and the caenogastropod species Pomacea canaliculata, which contrast with the uniform gene architectures of hemocyanins in Vetigastropoda. The goal of this study was to analyze hemocyanin gene evolution within the diverse group of Caenogastropoda in more detail. Our findings reveal multiple gene duplications and intron gains and imply that these represent general features of Apogastropoda hemocyanins. Whereas hemocyanin exon–intron structures are identical within different Tectipleura lineages, they differ strongly within Caenogastropoda among phylogenetic groups as well as between paralogous hemocyanin genes of the same species. Thus, intron accumulation took place more gradually within Caenogastropoda but finally led to a similar consequence, namely, a multitude of introns. Since both phenomena occurred independently within Heterobranchia and Caenogastropoda, the results support the hypothesis that introns may contribute to adaptive radiation by offering new opportunities for genetic variability (multiple paralogs that may evolve differently) and regulation (multiple introns). Our study indicates that adaptation of hemocyanin genes may be one of several factors that contributed to the evolution of the large diversity of Apogastropoda. While questions remain, this hypothesis is presented as a starting point for the further study of hemocyanin genes and possible correlations between hemocyanin diversity and adaptive radiation.

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Section: Discussionmentioning

confidence: 99%

The Evolution of Hemocyanin Genes in Caenogastropoda: Gene Duplications and Intron Accumulation in Highly Diverse Gastropods

et al. 2021

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“…Each FU involves one active site with a pair of copper atoms (Cu-A and Cu-B). These copper atoms reversibly capture O2 molecules, which bind as a peroxide ion (González et al, 2017). The native HlH is arranged by 20 subunits with 160 oxygen-binding active sites.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity of fractions isolated from hemolymph of garden snail Helix lucorum

Alexandrova¹,

Petrov²,

Dolashki³

et al. 2021

J Pharm Pharmacogn Res

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Context: The snail hemolymph is a multi-component mixture comprising various substances with pharmacological activity. Aims: To evaluate the antioxidant activity of hemocyanin and fractions isolated of hemolymph from garden snail Helix lucorum. Methods: The antioxidant activity was tested in chemical systems generating superoxide radicals (O2•–) and hydroxyl radicals (•OH) and was presented as percent inhibition. The fractions’ composition was analyzed by MALDI-TOF/TOF mass spectrometry. Results: At concentration of 90 µg/mL the hemolymph fraction with compounds with molecular weight (MW) <100kDa demonstrated the strongest O2•–– scavenger effect (82%), followed by the hemocyanin (58%), and hemolymph fraction with compounds with MW<1kDa (15%). The most powerful •OH scavenger effect at concentration of 90 µg/mL showed hemocyanin (43%) followed by MW<100kDa fraction (26%). No •OH scavenger effect was observed by the MW<1kDa fraction. The highest chelation properties (55%) were demonstrated by the MW<100kDa fraction at concentration of 130 µg/mL; both the hemocyanin and MW<1kDa hemolymph fraction showed 33% inhibitory effect at the same concentration. The analysis of the composition of the tested fractions revealed high content of hydrophobic surfaces in MW<1kDa fraction and presence of antioxidant enzymes-catalase, superoxide dismutase and glutathione peroxidase in MW<100kDa fraction that contribute to the overall antioxidant effect of H. lucorum hemolymph. Conclusions: The complex antioxidant effect of hemolymph from H. lucorum is thought to be related to the presence of low molecular weight peptides in the MW<1 kDa fraction including glutathione and mainly to the antioxidant enzymes in the MW<100 kDa fraction.

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“…The decamer is composed by self-assembly of ten subunits with molecular masses ranging from 350 kDa to 450 kDa depending on the species. The subunits contain seven to eight globular folded domains known as functional units (FUs), and each of them consists of α-helical domain that bears the active site and β-barrel domain [ 7 ]. The main recognized physiological functions of the hemocyanins are the binding, transportation, and delivery of oxygen from the respiratory organs to tissues.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model

et al. 2023

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Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the in vitro and in vivo anticancer activity of hemocyanins isolated from Helix aspersa, Helix lucorum, and Rapana venosa in the Graffi myeloid tumor model. The in vitro antitumor activity of the hemocyanins was determined by a MTT test and cytomorphological analysis by fluorescent and transmission electron microscopy. The in vivo effects of the hemocyanins were examined in hamsters transplanted with Graffi tumor. The serum antibody titers against the tested hemocyanins and tumor antigen were determined by ELISA. Histopathological assessment of the morphological features related to antitumor effect, immune system response, and toxicity in some internal organs was performed. The results of in vitro studies indicated that the tested hemocyanins induced significant antiproliferative and apoptogenic effects. The in vivo investigations demonstrated a protective antitumor effect, expressed in reduced transplantability, suppression of tumor growth and metastasis, reduced mortality, prolonged survival time, and absence of toxic side effects. The present study indicated that the antitumor activity of the studied hemocyanins was due to both immune stimulation and direct effects on the tumor cells, and they displayed their potential as therapeutic agents against hematological malignances.

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The oxygen-binding properties of hemocyanin from the mollusk Concholepas concholepas

Cited by 10 publications

References 44 publications

The Evolution of Hemocyanin Genes in Caenogastropoda: Gene Duplications and Intron Accumulation in Highly Diverse Gastropods

The Evolution of Hemocyanin Genes in Caenogastropoda: Gene Duplications and Intron Accumulation in Highly Diverse Gastropods

Antioxidant activity of fractions isolated from hemolymph of garden snail Helix lucorum

Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model

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