The P gene of the procine paramyxovirus LPMV encodes three possible polypeptides P, V and C: the P protein mRNA is edited

Berg, Mikael; Hjertner, Bernt; Moreno‐López, J.; Linné, Tommy

doi:10.1099/0022-1317-73-5-1195

Cited by 44 publications

(18 citation statements)

References 29 publications

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“…The expression of other proteins from overlapping reading frames was first shown in Sendai virus (SeV) (26,55). SeV (15,29), and Human parainfluenza virus type 1 (hPIV1) (8) express a set of four carboxy-coterminal proteins (CЈ, C, Y 1 , and Y 2 ), whereas the morbilliviruses express only a single C protein (6), and the rubulaviruses (e.g., Mumps virus and Simian virus 5 [SV5]), with the possible exception of La-Piedad-Michoacan-Mexico virus (7), do not express a C protein at all. The C proteins of MV (6) and SeV (67) have been reported to associate with the N and P proteins in infected cells, and the SeV C is found in purified virions (67).…”

Section: Rinderpest Virus (Rpv) Belongs To the Morbillivirus Genus Ofmentioning

confidence: 99%

Rinderpest Viruses Lacking the C and V Proteins Show Specific Defects in Growth and Transcription of Viral RNAs

Baron

Barrett

2000

J Virol

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Rinderpest virus is a morbillivirus and the causative agent of an important disease of cattle and wild bovids. The P genes of all morbilliviruses give rise to two proteins in addition to the P protein itself: use of an alternate start translation site, in a second open reading frame, gives rise to the C protein, while cotranscriptional insertion of an extra base gives rise to the V protein, a fusion of the amino-terminal half of P to a short, highly conserved, cysteine-rich zinc binding domain. Little is known about the function of either of these two proteins in the rinderpest virus life cycle. We have constructed recombinant rinderpest viruses in which the expression of these proteins has been suppressed, individually and together, and studied the replication of these viruses in tissue culture. We show that the absence of the V protein has little effect on the replication rate of the virus but does lead to an increase in synthesis of genome and antigenome RNAs and a change in cytopathic effect to a more syncytium-forming phenotype. Virus that does not express the C protein, on the other hand, is clearly defective in growth in all cell lines tested, and this defect appears to be related to a decreased transcription of mRNA from viral genes. The phenotypes of both individual mutant virus types are both expressed in the double mutant expressing neither V nor C.Rinderpest virus (RPV) belongs to the Morbillivirus genus of the family Paramyxoviridae and is thus related to Measles virus (MV), Canine and Phocid (seal) distemper viruses, the cetacean morbillivirus, and Peste des petits ruminants virus. The disease it causes (rinderpest) has for decades been one of the most widespread and economically important diseases affecting cattle in Africa, the Middle East, and the Indian subcontinent. It has been eliminated from most of these areas in recent years, largely through the efforts of the European Union-and Food and Agriculture Organization-backed EMPRES program, with the aim of global eradication by 2010 (22). Like all of the morbilliviruses, there is only one serotype of RPV, yet wide variation can be found in the pathogenicity of field isolates, from those causing essentially 100% mortality to others in which infection causes barely detectable clinical signs (58, 64). The molecular basis of this variation is unknown.All of the Paramyxoviridae have single-stranded RNA genomes of negative sense, with six viral genes, which are transcribed in order from a single promoter at the 3Ј end of the genome. From the second of those genes (the P gene), through utilization of more than one translation initiation codon and/or the introduction of one or more nontemplated residues to allow access to alternate reading frames, more than one protein is always produced, though the exact number and type of extra proteins vary both between and within genera. The expression of other proteins from overlapping reading frames was first shown in Sendai virus (SeV) (26, 55). SeV (15, 29), and Human parainfluenza virus type 1 (hPIV1) (8) expre...

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Section: Rinderpest Virus (Rpv) Belongs To the Morbillivirus Genus Ofmentioning

confidence: 99%

Rinderpest Viruses Lacking the C and V Proteins Show Specific Defects in Growth and Transcription of Viral RNAs

Baron

Barrett

2000

J Virol

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“…virus, the porcine rubulavirus La-Piedad-Michoacan-Mexico virus (Berg et al, 1992). We used the putative functional homologues NP and P, which also interact with each other in GST-PD analysis (unpublished results).…”

Section: Mapping the P40 Amino Acids Required For Interaction With P23mentioning

confidence: 99%

“…As a control antigen, another GST fusion protein, GST-P (Berg et al, 1992), was used to coat the plates. After blocking and washing of the plates, p23 was added at two different concentrations.…”

Section: Introductionmentioning

confidence: 99%

Two domains of the Borna disease virus p40 protein are required for interaction with the p23 protein.

Berg

Ehrenborg

Blomberg

et al. 1998

Journal of General Virology

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“…This virus was originally isolated in Mexico from brain tissue of a pig exhibiting clinical signs typical of LPMV infection 10 and has been characterized at protein and genomic levels. 1,2,4,11,12 Following receipt of this isolate at the Veterinary Sciences Division, the virus was purified by 3 passages at limiting dilution in continuous pig kidney cell cultures (PK/ 15), and a working pool was prepared. The infectious titer of this working pool was calculated to be 10 7.50 TCID 50 /0.1 ml, following inoculation of serial 10-fold dilutions in PK/ 15 cell cultures.…”

Section: Virusmentioning

confidence: 99%

A Sequential Study of Experimental Porcine Paramyxovirus (LPMV) Infection of Pigs: Immunostaining of Cryostat Sections and Virus Isolation

Allan¹,

McNeilly²,

Walker³

et al. 1996

J VET Diagn Invest

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Abstract. La Piedad Michoacan Paramyxovirus (LPMV) is a recently recognized paramyxovirus infecting pigs throughout Mexico. Disease syndromes observed in field cases associated with LPMV infection include neurologic, respiratory, and reproductive disorders. Clinical signs and the distribution of LPMV virus and antigen in tissue samples from pigs experimentally infected with LPMV by natural routes were studied. Severe neurologic disease and death occurred following experimental inoculation of 3-and 17-day-old pigs. All of the pigs inoculated at 3 days of age were either dead or moribund by 8 days after inoculation, whereas 30% of the pigs inoculated at 17 days of age were affected. Virus was consistently recovered from or demonstrated in tissues from the respiratory tract of both groups of pigs. LPMV and antigen were also demonstrated in central nervous system (CNS) tissues from these pigs; however, differences in virus distribution within the CNS were demonstrated in the 2 groups. In the pigs inoculated at 17 days of age, isolation of LPMV was restricted to the olfactory bulb and midbrain. In contrast, in the pigs inoculated at 3 days of age, isolation of LPMV was more widespread throughout the CNS tissue examined. Virus excretion studies indicated that nasal spread of LPMV was more important than fecal spread. Comparatively large quantities of infectious LPMV were consistently recovered from urine samples of experimentally infected pigs.

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The P gene of the procine paramyxovirus LPMV encodes three possible polypeptides P, V and C: the P protein mRNA is edited

Cited by 44 publications

References 29 publications

Rinderpest Viruses Lacking the C and V Proteins Show Specific Defects in Growth and Transcription of Viral RNAs

Rinderpest Viruses Lacking the C and V Proteins Show Specific Defects in Growth and Transcription of Viral RNAs

Two domains of the Borna disease virus p40 protein are required for interaction with the p23 protein.

A Sequential Study of Experimental Porcine Paramyxovirus (LPMV) Infection of Pigs: Immunostaining of Cryostat Sections and Virus Isolation

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