THE P<sup>k</sup>ANTIGEN AS RECEPTOR FOR THE HAEMAGGLUTININ OF PYELONEPHRITIC<i>ESCHERICHIA COLI</i>

Källenius, Gunilla; Möllby, R.; Svenson, Stefan B.; Winberg, Jan; Lundblad, Arne; Svensson, Sigfrid; Cedergren, B.

doi:10.1111/j.1574-6941.1980.tb01608.x

Cited by 380 publications

(57 citation statements)

References 14 publications

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“…Pyelonephritogenic E. coli may express various fimbriaecontaining adhesins that have been found to recognize different renal receptors and structures (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). P fimbriae associated with acute pyelonephritis provide an attachment mechanism to ␣ D-Gal-(1,4)-␤ -D-Gal-containing receptors residing on the luminal apical surface of the renal tubules (7,10,12,13).…”

Section: Introductionmentioning

confidence: 99%

“…P fimbriae associated with acute pyelonephritis provide an attachment mechanism to ␣ D-Gal-(1,4)-␤ -D-Gal-containing receptors residing on the luminal apical surface of the renal tubules (7,10,12,13). In contrast to P fimbriae, Dr fimbriae mediating the recognition of the receptors decay accelerating factor (DAF) 1 and collagen type IV, located in the tubular basement membranes and Bowman's capsule, may account for the renal interstitial tropism of E. coli (3,(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

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Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

et al. 1997

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

et al. 1997

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“…The saccharide chains show a cell type-specific distribution and constitute differentiation and tumor antigens (1,2) and receptors for microbial ligands. For instance, members of the globo-series GSLs [characterized by a Gal(al-4)Gal saccharide linkage] have been identified as stage-specific embryogenic antigens (3), as tumor antigens in Burkitt lymphoma (4), as receptors for Shiga toxin (5), and as receptors for uropathogenic Escherichia coli (6)(7)(8). The adherence ofE.…”

mentioning

confidence: 99%

Saccharide orientation at the cell surface affects glycolipid receptor function.

Strömberg

Nyholm

Pascher

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

174

114

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Three allelic variants of P-pilus-associated G-adheslns (ectins) with different cell-binding properties were recently described. Here we have analyzed Eschenchia cofl HB101 strains expressing the recombinant G-adhesin variants for their ability to agglutinate erythrocytes from various species as this relates to the glycosphingolipid (GSL) MATERIALS AND METHODSBacteria, Growth Conditions, and Labeling. E. coli strain HB101 was used to express the P pilus-associated G-adhesins PapGJ96, PrsGJ96, and PapGAD1LO carried by the recombinant plasmids pPAP5, pPAP601, and pPIL110-35, respectively (10). Plasmid pPAP5 consists of the vector pBR322 and a 9.7-kilobase (kb) EcoRI/BamHI chromosomal fragment derived from E. coli J96 and encodes F13 P pili with the PapGJ96 adhesin (10). Plasmid pPAP601 is a pACYC184 derivative carrying another E. coli J96 chromosomal fragment encoding serologically identical F13 pili but having the PrsGJ% adhesin (10). Plasmid pPIL110-35 is a pACYC184 derivative containing a 16-kb EcoRI fragment of E. coli AD110 and encoding F72 P pili with the PapGAD110 adhesin (10).For the glycolipid binding assay, the bacteria were cultivated overnight at 37TC on Luria agar plates (10) supplemented with carbenicillin, chloramphenicol, and tetracycline (100, 20, and 10 jig/ml, respectively). Metabolically labeled bacteria were obtained by addition of [35S]methionine (600 Ci/mmol; 150-200 ,uCi per plate; 1 Ci = 37 GBq; Amersham). After growth, the bacteria were harvested by centrifugation, washed twice in phosphate-buffered saline (PBS) (pH 7.3), and resuspended in PBS.Hemagglutination Assay. For hemagglutination, 10 of fresh bacteria (109 cells per ml) and 10 of erythrocyte suspension (4%), both suspended in PBS at pH 7.3, were mixed on a glass slide. During gentle mixing for 5 min at room temperature, agglutination was visualized and scored (see Table 1).Erythrocyte Glycolipids. For the preparation of total acidic and neutral GSLs (10), fresh erythrocytes were washed twice in saline and 100 ml of erythrocyte concentrate was shaken continuously together with toluene and water (65 ml and 30 ml, respectively). After centrifugation (10,000 x g for 5 min), the purified membranes (10 g) at the toluene/water interface were extracted twice with methanol (100 ml), once with chloroform/methanol (2:1) (100 ml), and once with methanol (100 ml) for 25 min at 70TC. 9340The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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“…E. coli P-fimbriae associated with urinary tract infections can be subdivided into serologically distinct groups termed F7-Fl4 [1][2][3]. All of them recognize as receptor the a-o-Gal-(1-4)-ß-oGal region of globotetraosylceramide and trihexosyl-ceramide, which are antigens of the human blood group P system [4]. E. coli exhibiting P fimbriae produce a mannose-resistant hemagglutination (MRH) with human erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal mapping of genes encoding mannose-sensitive (type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli O18:K5:H5

Krallmann-Wenzel¹

1989

FEMS Microbiology Letters

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THE P^kANTIGEN AS RECEPTOR FOR THE HAEMAGGLUTININ OF PYELONEPHRITICESCHERICHIA COLI

Cited by 380 publications

References 14 publications

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

Saccharide orientation at the cell surface affects glycolipid receptor function.

Chromosomal mapping of genes encoding mannose-sensitive (type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli O18:K5:H5

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THE PkANTIGEN AS RECEPTOR FOR THE HAEMAGGLUTININ OF PYELONEPHRITICESCHERICHIA COLI

Cited by 380 publications

References 14 publications

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

Saccharide orientation at the cell surface affects glycolipid receptor function.

Chromosomal mapping of genes encoding mannose-sensitive (type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli O18:K5:H5

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THE P^kANTIGEN AS RECEPTOR FOR THE HAEMAGGLUTININ OF PYELONEPHRITICESCHERICHIA COLI