Abstract:Ectopically expressing viral suppressors of RNA silencing (VSR) in transgenic Arabidopsis overcome host range limitation and viral titer variation, and have similar VSR levels to investigate RNA silencing suppression. Therefore, identifying the insertion of the transgenic VSR gene and developing an endogenous AGO1-based in vitro RNA-inducing silencing complex (RISC) assay prompted further investigation into VSR-mediated suppression. Three P1/HC-Pro plants from turnip mosaic virus (TuMV) (P1/HC-ProTu), zucchini… Show more
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