The Pagetoid Variant of Urothelial Carcinoma In Situ of Urinary Bladder in a Cow

Abstract

A case of urothelial carcinoma in situ of urinary bladder is reported in a 10-year-old cow naturally grazing on bracken-infested land. The cow suffered from enzootic hematuria for more than 5 years. The presence of bovine papillomavirus type 2 (BPV-2) DNA sequences was detected by polymerase chain reaction. The carcinoma in situ was characterized by the presence of anaplastic cells with amphophilic cytoplasm and pleomorphic nuclei containing granular, irregularly dispersed chromatin. Focal areas within the tumor contained large isolated and/or clustered cells. These cells had pale acidophilic cytoplasm, large nuclei with single or multiple nucleoli, and well-defined borders resembling Paget's cells. Immunohistochemically, all malignant cells were negative for vimentin and S-100 and positive for cytokeratins. In addition, normal and neoplastic cells expressed fragile histidine triad (FHIT) protein; surprisingly, some pagetoid cells did not. FHIT, the tumor suppressor gene at 3p14.2, encodes a protein of 147 amino acids (16.8 kd) with diadenosine triphosphate hydrolase activity and is a common target of deletions in human cancers of epithelial origin. Antibody to laminin detected a continuous epithelial basement membrane, thus clearly showing that neoplastic changes were limited to urothelial cells without invading stromal tissue. To our knowledge, this is the first report of an unusual pattern of spread of urothelial carcinoma in situ in a cow.

Enfermidades determinadas pelo princípio radiomimético de Pteridium aquilinum (Polypodiaceae)

“…Dez por cento dos carcinomas metastizaram para os linfonodos ilíacos e, em três animais, também para o pulmão. Adicionalmente foram verificados carcinoma papilar, angios-sarcoma (Pamukcu 1955), hemangioendotelioma (Tsumura et al 1972) e carcinoma transicional variante pagetóide (Borzacchiello et al 2001).…”

Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows

“…A possible aetiopathogenic mechanism is that BPV-1/2 infect the epithelium of the bladder and produce an abortive or latent infection, as already hypothesized for BPV-4 in gastrointestinal tumours (Borzacchiello et al, 2003;Campo et al, 1994). The immunosuppressants and chemical carcinogens in bracken act synergistically with the virus to induce the formation of pre-neoplastic lesions.…”

“…The immunosuppressants and chemical carcinogens in bracken act synergistically with the virus to induce the formation of pre-neoplastic lesions. During neoplastic progression, the ras gene is activated (Campo et al, 1990) and expression of the tumour suppressor fragile histidine tetrads locus is downregulated (Borzacchiello et al, 2001).…”

“…PCR amplification of viral sequences using consensus primers was done according to methods described previously (Borzacchiello et al, 2001). These primers allow the amplification of a 307 bp DNA amplicon encompassing the BPV E5 sequence.…”

Análise filogenética de papilomavírus bovino associado com lesões cutâneas em rebanhos do Estado do Paraná

“…Todos os tipos de BPV já foram identificados em papilomas cutâneos presentes em várias partes do corpo, incluindo os tetos e úbere (Bloch et al 1997, Campo 1998, Jelínek & Tachezy, 2005. Lesões neoplásicas na bexiga e no trato digestório superior de bovinos também são associadas com a infecção pelo BPV-2 e BPV-4, respectivamente, em associação com cofatores oncogênicos presentes na samambaia (Pteridium aquilinum) (Campo et al 1992, Borzacchiello et al 2001.…”