The Pan African Vivax and Ovale Network (PAVON): Refocusing on Plasmodium vivax, ovale and asymptomatic malaria in sub-Saharan Africa

Quaye, Isaac; Aleksenko, Larysa; Oeuvray, Claude; Yewhalaw, Delenasaw; Duah, Nancy O.; Gyan, Ben; Haiyambo, Daniel H.; Dongho, Gryslaine Bruna Djeunang; Ayanful-Torgby, Ruth; Amoah, Linda Eva; Hamid, Mahdi Abdel; Worku, Solomon; Bahiti, Assefa Ashenafi; Pasquale, Harriet; Bitshi, Mimie; Troare, Isidore; Diarra, Amidou; Njunju, Eric M.; Cissé, Mamoudou; Soulama, Issiaka; Savadogo, Ragnessi Justin; Issifou, Saadou; Niangaly, Amadou; Dembélé, Laurent; Greco, Béatrice

doi:10.1016/j.parint.2021.102415

Cited by 11 publications

(12 citation statements)

References 59 publications

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“…; Obaldia et al., 2018 ; Silva-Filho et al., 2020 ; Kho et al., 2021 ). There are an increasing number of reports of P. vivax infections now being detected with sensitive tools in Sub-Saharan Africa where it was long thought to have been absent due to the high proportion of Duffy-negative phenotype in the population ( Culleton et al., 2009 ; Oboh et al., 2020 ; Quaye et al., 2021 ; Wilairatana et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, recent studies suggest a much more widespread reservoir of P. vivax transmission, with a potential role for extravascular niches where the parasite can survive while it is not detected (Dechavanne et al;Obaldia et al, 2018;Silva-Filho et al, 2020;Kho et al, 2021). There are an increasing number of reports of P. vivax infections now being detected with sensitive tools in Sub-Saharan Africa where it was long thought to have been absent due to the high proportion of Duffy-negative phenotype in the population (Culleton et al, 2009;Oboh et al, 2020;Quaye et al, 2021;Wilairatana et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Novel highly-multiplexed AmpliSeq targeted assay for Plasmodium vivax genetic surveillance use cases at multiple geographical scales

Kattenberg

Nguyen

et al. 2022

Front. Cell. Infect. Microbiol.

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Although the power of genetic surveillance tools has been acknowledged widely, there is an urgent need in malaria endemic countries for feasible and cost-effective tools to implement in national malaria control programs (NMCPs) that can generate evidence to guide malaria control and elimination strategies, especially in the case of Plasmodium vivax. Several genetic surveillance applications (‘use cases’) have been identified to align research, technology development, and public health efforts, requiring different types of molecular markers. Here we present a new highly-multiplexed deep sequencing assay (Pv AmpliSeq). The assay targets the 33-SNP vivaxGEN-geo panel for country-level classification, and a newly designed 42-SNP within-country barcode for analysis of parasite dynamics in Vietnam and 11 putative drug resistance genes in a highly multiplexed NGS protocol with easy workflow, applicable for many different genetic surveillance use cases. The Pv AmpliSeq assay was validated using: 1) isolates from travelers and migrants in Belgium, and 2) routine collections of the national malaria control program at sentinel sites in Vietnam. The assay targets 229 amplicons and achieved a high depth of coverage (mean 595.7 ± 481) and high accuracy (mean error-rate of 0.013 ± 0.007). P. vivax parasites could be characterized from dried blood spots with a minimum of 5 parasites/µL and 10% of minority-clones. The assay achieved good spatial specificity for between-country prediction of origin using the 33-SNP vivaxGEN-geo panel that targets rare alleles specific for certain countries and regions. A high resolution for within-country diversity in Vietnam was achieved using the designed 42-SNP within-country barcode that targets common alleles (median MAF 0.34, range 0.01-0.49. Many variants were detected in (putative) drug resistance genes, with different predominant haplotypes in the pvmdr1 and pvcrt genes in different provinces in Vietnam. The capacity of the assay for high resolution identity-by-descent (IBD) analysis was demonstrated and identified a high rate of shared ancestry within Gia Lai Province in the Central Highlands of Vietnam, as well as between the coastal province of Binh Thuan and Lam Dong. Our approach performed well in geographically differentiating isolates at multiple spatial scales, detecting variants in putative resistance genes, and can be easily adjusted to suit the needs in other settings in a country or region. We prioritize making this tool available to researchers and NMCPs in endemic countries to increase ownership and ensure data usage for decision-making and malaria policy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel highly-multiplexed AmpliSeq targeted assay for Plasmodium vivax genetic surveillance use cases at multiple geographical scales

Kattenberg

Nguyen

et al. 2022

Front. Cell. Infect. Microbiol.

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“…The prevalence of P. ovale spp. is probably underestimated due to low parasite densities and difficulties in detection by microscopy [2,3].…”

Section: Introductionmentioning

confidence: 99%

Disparate selection of mutations in the dihydrofolate reductase gene (dhfr) of Plasmodium ovale curtisi and P. o. wallikeri in Africa

Chen

Tang

et al. 2022

PLoS Negl Trop Dis

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Plasmodium ovale curtisi and P. ovale wallikeri are both endemic in sub-Saharan Africa, the Middle East and Southeast Asia. Molecular surveillance data for drug resistance in P. ovale spp. is limited at present. We analysed polymorphisms in the podhfr, pocrt and pocytb genes of P. ovale spp. in 147 samples collected from travelers returning to China from Africa. Two podhfr mutations, S58R and S113N/T were detected in P. ovale curtisi with high/moderate frequencies of 52.17% and 17.39%, respectively. Evidence of positive selection (dN/dS = 2.41) was found for podhfr in P. ovale curtisi and decreased diversity (He) of microsatellite markers flanking the mutant alleles suggests that selective sweeps have occurred for both. Mutations E34G (1.50%) and L43V (1.50%) in pocrt of P. ovale curtisi, and E34G (3.70%), I102M (1.80%) and V111F (1.80%) of P. ovale wallikeri were found at low frequencies. Mutations R66K (6.20%), R75K (11.63%) and R95K (3.88%) of pocytb were found in both P. ovale curtisi and P. ovale wallikeri. These results suggest that the podhfr gene of P. ovale curtisi may be subject to drug selection in Africa, warranting further attention. We observed significant differences in the prevalence and distribution of podhfr mutations between the two P. ovale species, suggestive of fundamental biological differences between them.

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“…Malaria caused by P. vivax is being diagnosed with increasing frequency in Africa. 1 Some southern countries where it has been detected are Angola, Botswana, Mozambique, Namibia, Zambia and Zimbabwe. 1 Knowing the parasite origin (mosquito or human tissue) of P. vivax infection recurrences (which can be reinfections, recrudescences or relapses) is important epidemiologically for malaria elimination in Africa.…”

mentioning

confidence: 99%

“…1 Some southern countries where it has been detected are Angola, Botswana, Mozambique, Namibia, Zambia and Zimbabwe. 1 Knowing the parasite origin (mosquito or human tissue) of P. vivax infection recurrences (which can be reinfections, recrudescences or relapses) is important epidemiologically for malaria elimination in Africa. A reason is that the efficacy of drugs against parasites might vary according to their location in the body.…”

mentioning

confidence: 99%

Theoretical origin of genetically homologous Plasmodium vivax malarial recurrences

Markus

2022

Southern African Journal of Infectious Diseases

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Malaria caused by Plasmodium vivax is being diagnosed with increasing frequency in Africa. Some southern countries where it has been detected are Angola, Botswana, Mozambique, Namibia, Zambia and Zimbabwe. Knowing the parasite origin of P. vivax infection recurrences (which can be reinfections, recrudescences or relapses) is important epidemiologically for malaria elimination in Africa. Although hypnozoites will no doubt be a source, we should try to determine how frequently the origin of non-reinfection recurrences of P. vivax malaria involving closely related parasites may be non-circulating merozoites rather than hypnozoites.

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The Pan African Vivax and Ovale Network (PAVON): Refocusing on Plasmodium vivax, ovale and asymptomatic malaria in sub-Saharan Africa

Cited by 11 publications

References 59 publications

Novel highly-multiplexed AmpliSeq targeted assay for Plasmodium vivax genetic surveillance use cases at multiple geographical scales

Novel highly-multiplexed AmpliSeq targeted assay for Plasmodium vivax genetic surveillance use cases at multiple geographical scales

Disparate selection of mutations in the dihydrofolate reductase gene (dhfr) of Plasmodium ovale curtisi and P. o. wallikeri in Africa

Theoretical origin of genetically homologous Plasmodium vivax malarial recurrences

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