The pancreatic duct epithelium in vitro: Bile acid injury and the effect of epidermal growth factor

Álvarez, Carlos; Nelms, Cynthia; D'Addio, Victor; Bass, Barbara L.

doi:10.1016/s0039-6060(97)90041-6

Cited by 13 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has also been reported that EGF promotes the growth of pancreatic duct cells in pigs [28] and mice [21]. On the other hand, an in vitro study has reported that EGF promotes the cell division of guinea pig [27], bovine [1] and canine pancreatic duct cells [31], isolated mouse pancreatic acinar cells [18], and isolated rat islet cells [4]. However, it has also been reported that in a low concentration (2.8 mM) of glucose, the action of EGF on isolated rat islets did not cause a change in the incorpora- tion of 3 H-thymidine [4].…”

Section: Discussionmentioning

confidence: 98%

Effects of Epidermal Growth Factor (EGF) on Fetal Islet B Cells in vitro.

Yasuda

Yamamoto

Arishima

et al. 2002

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. It has been reported that when the rat fetus is treated with streptozotocin (STZ) in vivo, islet B cells are destroyed but later recover. To investigate the process of the recovery of B cells after in vitro treatment of the fetal pancreas with STZ and the role of epidermal growth factor (EGF) in the recovery of B cells, we measured the level of insulin released from the cultured fetal pancreas and examined it histologically. As a result, we immunohistologically confirmed the regeneration of B cells in the pancreas that had been cultured for 48 hr after destruction of islet B cells by STZ treatment. An immunohistologic study using proliferating cell nuclear antigen (PCNA) showed that without the addition of EGF, the cell division index was significantly higher in the STZ-treated group (STZ group) than in the untreated group (intact group), whereas with the addition of EGF, the cell division index increased in both groups, but EGF did not have a significant cell division-promoting effect on the pancreas in the STZ group. The addition of EGF caused a significant decrease in the concentration of insulin in culture medium in both groups. These results indicate that EGF has a cell growth-promoting effect on intact fetal pancreas in vitro but has the effect of inhibiting the release of insulin, and thus suggest that EGF does not trigger the regeneration of islet B cells. KEY WORDS: epidermal growth factor (EGF), fetal islet B cell, insulin, proliferating cell nuclear antigen (PCNA), streptozotocin (STZ).J. Vet. Med. Sci. 64(2): 101-105, 2002 Epidermal growth factor (EGF) is a polypeptide of molecular weight 6,045 originally extracted from the submaxillary glands of mice and accelerates eyelid opening and incisor eruption in newborn mice [5]. EGF has also been reported to function in accelerating the growth of the gastrointestinal mucosal epithelium, inhibiting the secretion of gastric acid, and protecting the gastric mucosa [6,10].EGF has been shown to affect the growth and differentiation of cultured cells derived from various organs [3], and reportedly has in vivo and in vitro growth-accelerating effects on the pancreas [4]. However, these in vitro studies used the isolated exocrine part or pancreatic islets, and not intact pancreases in organ culture.EGF receptors are present in various cells [3], and their presence in the pancreas has also been confirmed [7,15,19]. From the observation that EGF occurs in pancreatic tissue and pancreatic juice [12][13][14], it is postulated that EGF has some effect on the pancreas itself.Streptozotocin (STZ) is an experimental diabetogenic agent that causes diabetes mellitus by specifically destroying islet B cells [23]. Its toxic action on islet B cells is irreversible. However, it has been reported that perinatal rats subjected to STZ treatment reversibly recover from STZinduced diabetes mellitus [2,22]. It has also been reported that the direct administration of STZ to the rat fetus immediately decreases the total mass of the islet B cells, which is markedly restored...

show abstract

Section: Discussionmentioning

confidence: 98%

Effects of Epidermal Growth Factor (EGF) on Fetal Islet B Cells in vitro.

Yasuda

Yamamoto

Arishima

et al. 2002

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…In humans, high concentrations of bile are toxic and increase the production of free radicals in the liver, which can cause inflammation and tissue damage (Ljubuncic et al, 2000). In cattle, high concentrations of bile cause tissue damage to bovine pancreatic duct epithelial cells in culture (Alvarez et al, 1997). Increased concentrations of free radicals in the liver of cows are indicated by elevated concentrations of malondialdehyde, one of the end products of lipid peroxidation, and decreased concentrations of α-tocopherol (Mudron et al, 1999), an antioxidant, in plasma (Table 3) and to a smaller extent in the liver.…”

Section: Histological and Metabolic Pathology Of Fatty Livermentioning

confidence: 99%

Invited Review: Pathology, Etiology, Prevention, and Treatment of Fatty Liver in Dairy Cows

Bobe

Young

Beitz

2004

Journal of Dairy Science

703

714

View full text Add to dashboard Cite

Fatty liver (i.e., hepatic lipidosis) is a major metabolic disorder of many dairy cows in early lactation and is associated with decreased health status and reproductive performance. In severe cases, milk production and feed intake are decreased. Therefore, a practical preventative or an efficacious treatment of fatty liver could save millions of dollars yearly in treatment, replacement, and production losses for dairy farmers. Fatty liver develops when the hepatic uptake of lipids exceeds the oxidation and secretion of lipids by the liver, which usually is preceded by high concentrations of plasma NEFA mobilized from adipose tissue. Excess lipids are stored as triacylglycerol in the liver and are associated with decreased metabolic functions of the liver. Liver can be categorized into normal liver or mild, moderate, or severe fatty liver; the latter can be subdivided further into nonencephalopathic severe fatty liver and hepatic encephalopathy. Insufficient or unbalanced dietary intake, obesity, and elevated estrogen concentrations are involved in the etiology of fatty liver, which is associated with greater incidence of dystocia, diseases, infections, and inflammations. Because even mild fatty liver is associated with decreased health status and reproductive performance of dairy cows, prevention of fatty liver by supplying cows with sufficient nutrients and a clean and health-promoting environment in the peripartal period would reduce production losses of cows more than would any treatment of fatty liver. This, however, might not be enough for cows that are obese or do not eat well, had calving difficulties or twins, have metabolic or infectious diseases, or are in severe negative energy balance because of high milk production immediately after calving. Potential and commonly used preventatives, as well as treatments, are discussed in the review. Currently, detection of fatty liver is possible only by minor surgery. Ultrasonic techniques offer a potential tool to noninvasively detect fatty liver. Future gene-array and proteomic studies may provide means to detect early molecular events in the etiology of fatty liver plus their connection with immune function and reproductive performance so that more effective treatments and preventatives of fatty liver can be developed. Such advances hopefully will make fatty liver a problem of the past.

show abstract

“…Contrary to the effects of low concentrations of bile acids, high concentrations of CDC lead to epithelial barrier damage, 606566 the secretory mechanisms of pancreatic ductal cells are inhibited and the ducts can no longer act as a defensive wall against the toxic bile. Notably, longer administration of high concentrations of bile acids can cause a detergent-like, uncontrolled increase in plasma membrane H + permeability 60 ; and if this occurs, cell injury and death cannot be prevented.…”

Section: Effects Of Bile Acids In the Exocrine Pancreasmentioning

confidence: 99%

The acinar-ductal tango in the pathogenesis of acute pancreatitis

Hegyi

Pandol

Venglovecz

et al. 2010

Gut

103

View full text Add to dashboard Cite

There is an unacceptably high mortality in acute pancreatitis, which is due to the lack of specific treatments for the disease. A major reason stated to account for the inability to develop effective treatments is that there are multiple pathobiologic pathways activated in the acinar cell mediating pancreatitis making it difficult to choose molecular targets for therapeutic strategies. However, this reasoning limits opportunities for therapeutic development because it does include another important participant in pancreatitis - the pancreatic duct cells. The most recent advance in pancreatitis research is that depletion of both glycolytic and oxidative ATP synthesis is a common event in both acinar and ductal cells. Although ATP has a very short half-life in the blood and is hydrolysed to ADP, there is clear evidence that encapsulating ATP into liposomes can effectively drive ATP into the cells which can be effective in protecting them from necrosis. In this review, we will examine the effects of different insults associated with pancreatitis on both the acinar and ductal components of the exocrine pancreas pointing out the role of the ductal epithelial responses in both attenuating and increasing the severity of pancreatitis. In addition, we propose that exogenous ATP administration may restore ductal and acinar function providing therapeutic benefit.

show abstract

The pancreatic duct epithelium in vitro: Bile acid injury and the effect of epidermal growth factor

Cited by 13 publications

References 22 publications

Effects of Epidermal Growth Factor (EGF) on Fetal Islet B Cells in vitro.

Effects of Epidermal Growth Factor (EGF) on Fetal Islet B Cells in vitro.

Invited Review: Pathology, Etiology, Prevention, and Treatment of Fatty Liver in Dairy Cows

The acinar-ductal tango in the pathogenesis of acute pancreatitis

Contact Info

Product

Resources

About