The parameters of the porcine eyeball

Sánchez, Irene; Martín, Raúl; Ussa, Fernando; Fernandez-Bueno, Iván

doi:10.1007/s00417-011-1617-9

Cited by 237 publications

(156 citation statements)

References 30 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…The application of the method developed during studies in pigs in human cases and its reliability can be explained by the similar anatomical structure and location of pig and human eyeballs [11] and therefore probably of similar thermodynamic properties. The additional advantages of the currently presented method is lack of significant influence of clothes and body mass on the cooling rate of the eyeballs.…”

Section: Resultsmentioning

confidence: 99%

First practical applications of eye temperature measurements for estimation of the time of death in casework. Report of three cases

Kaliszan

2012

Forensic Science International

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

First practical applications of eye temperature measurements for estimation of the time of death in casework. Report of three cases

Kaliszan

2012

Forensic Science International

View full text Add to dashboard Cite

“…In our previous work with pig eyes and the study presented here, we took advantage of the high tissue quality that is the result of only two hours from enucleation to culture, the consistency within a litter, and an outflow tract anatomy that matches several features in humans [15][16][17][18] . Notable differences are a thicker TM, Schlemm's canal-like segments instead of a mostly single lumen (angular aqueous plexus) 19 , and, in contrast to almost all other domestic animals and pets 20 , a paucity of naturally developing glaucoma or medically-induced ocular hypertension.…”

Section: Introductionmentioning

confidence: 99%

A Porcine Ex Vivo Model of Pigmentary Glaucoma

Dang¹,

Waxman²,

Wang³

et al. 2018

Preprint

View full text Add to dashboard Cite

Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo model that recreates several features of pigmentary glaucoma, including intraocular hypertension, accumulation of pigment in the trabecular meshwork, and declining phagocytosis. We found that trabecular meshwork cells regulate outflow, form actin stress fibers, and have a decreased phagocytic activity. Gene expression microarrays and a pathway analysis of TM monolayers as well as ex vivo anterior segment perfusion cultures indicated that RhoA plays a central role in regulating the cytoskeleton, motility, and phagocytosis in the trabecular meshwork, providing new insights and targets to investigate in pigmentary glaucoma.

show abstract

“…As the average axial length of porcine eyes is similar to that of humans, a flatter cornea and thicker lens imply a smaller average vitreous volume (3.5 vs. 4.5 mL) for porcine versus human eyes, respectively. 19,20 Some eyes were pretreated with V20I 24 hours before injection with iomeprol to mimic age-related syneresis in the human vitreous body. 21 …”

Section: Injected Solutionsmentioning

confidence: 99%

Intravitreally Injected Fluid Dispersion: Importance of Injection Technique

Willekens

Reyns²,

Diricx³

et al. 2017

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. The purpose of this study was to evaluate the dispersion of intravitreally injected solutions and investigate the influence of varying injection techniques.METHODS. This was a prospective study using enucleated porcine eyes and ultra-highresolution computed tomography (UHRCT) scanning to visualize iomeprol intravitreal dispersion. Sixty eyes were divided over 12 different groups according to the injection procedure: fast (2 seconds) or slow (10 seconds) injection speed and needle tip location (6-and 12-mm needle shaft insertion or premacular tip placement verified by indirect ophthalmoscopy). For each of these combinations, eyes were either injected with the combination of V20I (which is an analogue of ocriplasmin) and iomeprol or iomeprol alone. Distance to the macula and volume measurements were performed at 1, 2, 3, and 5 hours after injection.RESULTS. The measured contrast bolus volume increases slowly over time to an average of 0.70 (P ¼ 0.03), 1.04 (P ¼ 0.006), and 0.79 (P ¼ 0.0001) cm 3 5 hours after the injection for the 6-mm needle shaft insertion, 12-mm needle shaft insertion, and premacular needle tip placement, respectively. The distance to the macular marker was significantly lower for premacular needle tip placement injections compared with 6-and 12-mm needle shaft insertion depths.CONCLUSIONS. Ultra-high-resolution computed tomography with three-dimensional reconstruction offers the possibility to study the dispersion of intravitreally injected solutions in a noninvasive manner. Intravitreal premacular solution delivery is possible with an indirect ophthalmoscope-guided injection technique and significantly reduces the time to reach the posterior pole in respect to 6-and 12-mm needle insertion depths. The speed of injection does not influence dispersion significantly.

show abstract

The parameters of the porcine eyeball

Abstract: This paper describes the anatomy of the pig eyeball for easy use and interpretation by researchers who are considering their choice of animal model in vision sciences research.

Cited by 237 publications

References 30 publications

First practical applications of eye temperature measurements for estimation of the time of death in casework. Report of three cases

First practical applications of eye temperature measurements for estimation of the time of death in casework. Report of three cases

A Porcine Ex Vivo Model of Pigmentary Glaucoma

Intravitreally Injected Fluid Dispersion: Importance of Injection Technique

Contact Info

Product

Resources

About