The partial purification of parathyroid hormone by means of ultrafiltration and displacement chromatography

Rasmussen, Howard; Westall, R. G.

doi:10.1042/bj0670658

Cited by 25 publications

(9 citation statements)

References 5 publications

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“…Years of relative neglect of fundamental PTH research ended with a report by Rasmussen and Westall [9] that highly purified biologically active material could be obtained by extracting glands in hot acetic acid, but this preparation was not completely stable. In 1959, Aurbach [10] first reported that phenol extraction of bovine parathyroid glands yielded a biologically active preparation of reasonable potency.…”

Section: Modern Historymentioning

confidence: 98%

Present at the beginning: a personal reminiscence on the history of teriparatide

Marcus

2011

Osteoporos Int

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The ability of parathyroid glandular extracts to stimulate bone acquisition in rodents was established in the 1920s, but interest in this action lay dormant for almost 50 years until application of contemporary laboratory methods permitted the large-scale production of an amino-terminal fragment of PTH, (1-34) hPTH (teriparatide), which was capable of carrying out all known actions of the full-length (1-84) PTH molecule. In the 1970s, largely stimulated by the efforts of a British pharmacologist, Dr. John Parsons, the scientific community began to revisit these anabolic actions and showed that single daily injections of teriparatide dramatically increased bone mass in several mammalian species and restored bone in oöphorectomized rats. Shortly thereafter, human studies confirmed a striking increase in trabecular bone mass and showed also that an important part of teriparatide's action is to increase cortical bone. Eli Lilly and Company conducted a formal registration trial in postmenopausal women with osteoporosis. The unexpected occurrence of osteosarcomas in Fisher 344 rats treated long-term with teriparatide provoked an abrupt cessation of that trial, but ambiguity concerning the relevance of this rat finding to human disease, combined with significant anti-fracture efficacy, led to FDA approval of teriparatide for men and postmenopausal women with osteoporosis "at high risk for fracture" in 2002. Subsequently, teriparatide has been approved also for treatment of patients with glucocorticoid-associated osteoporosis, and papers indicating utility of this agent for dental and orthopedic applications have begun to appear.

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Section: Modern Historymentioning

confidence: 98%

Present at the beginning: a personal reminiscence on the history of teriparatide

Marcus

2011

Osteoporos Int

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“…Preparation and test of extracts of bovine superior parathyroid glands for hypocalcaemic potency Dried, defatted bovine parathyroid powder was prepared according to the method of Rasmussen & Westall (1957). This powder was then treated with 8M-urea and 0-1 M-cysteine in 0-2N-hydrochloric acid (Rasmussen, Sze & Young, 1964).…”

Section: (B) Thyroid Lobe With and Without Inferior Parathyroid Glandmentioning

confidence: 99%

An Investigation Into the Parathyroid Origin of Calcitonin

Care¹,

Keynes²,

Duncan³

1966

Journal of Endocrinology

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To investigate the part played by the parathyroids as a source of calcitonin under controlled conditions, conscious, thyroidectomized animals have been used, in which a superior parathyroid was perfused with blood of variable composition and parathyroid venous blood could be collected at will.Hypercalcaemic perfusion of such a parathyroid gland usually resulted in a prompt systemic hypocalcaemic response but it was impossible to conclude with certainty that this was not merely due to the cessation of parathyroid hormone secretion. Although no effect was observed in acute experiments, cross-transfusion of parathyroid venous plasma during hypercalcaemic perfusion of the gland in conscious sheep was followed by a small hypocalcaemic response in the two recipient lambs. The time-course of this response was similar to that obtained in sheep following the intravenous injection of extracts of bovine superior parathyroid glands. However, pure parathyroid hormone, injected intravenously into a sheep, was also found to cause an initial hypocalcaemia, followed later by hypercalcaemia.In general, similar changes in plasma magnesium concentration accompanied those of plasma calcium concentration as a result of hypercalcaemic perfusion of a parathyroid gland or the intravenous injection of bovine parathyroid extracts.It is impossible to conclude with certainty that, in the sheep, the parathyroid glands secrete calcitonin in response to the stimulus of hypercalcaemia, although the possibility cannot be entirely eliminated.

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“…Displacement chromatography is a technique that has been successfully utilized for the enrichment and isolation of trace variants in peptide digests [9][10][11][12], as well as high-resolution separation of proteins [13][14][15][16], oligonucleotides [17,18] and small organic molecules [19,20]. As such, displacement chromatography provides a powerful but underutilized tool in the process development and characterization of complex biological therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of therapeutic antibody charge variants using cation exchange displacement chromatography

Zhang

Bourret

Cano³

2011

Journal of Chromatography A

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The partial purification of parathyroid hormone by means of ultrafiltration and displacement chromatography

Cited by 25 publications

References 5 publications

Present at the beginning: a personal reminiscence on the history of teriparatide

Present at the beginning: a personal reminiscence on the history of teriparatide

An Investigation Into the Parathyroid Origin of Calcitonin

Isolation and characterization of therapeutic antibody charge variants using cation exchange displacement chromatography

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