The partial thromboplastin (cephalin) time test

Poller, L.; Thomson, Jean M.

doi:10.1136/jcp.25.12.1038

Cited by 27 publications

(14 citation statements)

References 6 publications

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“…The results were calculated from the respective calibration curves obtained using serial dilutions of a single batch of normal pooled plasma (lyophilized preparation prepared at the National (UK) Reference Laboratory for Anticoagulant Reagents and Control). Laboratory standardized method (Poller 8: Thomson, 1972).…”

Section: Methodsmentioning

confidence: 99%

An Evaluation of Chromogenic Substrates in the Control of Oral Anticoagulant Therapy

1981

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A comparison has been made between the prothrombin time test using British Comparative Thromboplastin (BCT) and four modified amidolytic assays in the assessment of laboratory control of oral anticoagulant administration in short-term and long-term patients. The results were also correlated with the APTT and specific assays for factors VII and X based on established coagulation techniques. The chromogenic substrate assays did not give as close agreement with the BCT as the clotting assays for factors VII and X. A combination of the amidolytic factor II and X results did not improve the correlation. Agreement with the BCT was better in long-term patients with the substrates but there was no obvious advantage between the factor II and X amidolytic methods in this group. The development of a specific amidolytic assay for factor VII should be considered based on the evidence of this study although its reliability for oral anticoagulant control would need to be validated by clinical trial. In the interim, it may be anticipated that thromboplastins showing less sensitivity than BCT to factor VII would give a better correlation with the amidolytic assays.

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Section: Methodsmentioning

confidence: 99%

An Evaluation of Chromogenic Substrates in the Control of Oral Anticoagulant Therapy

1981

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“…12886) prepared from human placenta (Behring Institute, Marburg, West Germany). Activating agents were used in the following concentrations according to directions (Poller and Thomson 1972) ; 0.17% kaolin (Wako Pure Chemical Industries, LTD., Osaka), 0.17% celite (Wako Pure Chemical Industries, LTD., Osaka) and 0.01% ellagic acid (bade Diagnostics, Inc., Miami USA).…”

Section: Methodsmentioning

confidence: 99%

“…They were also incubated only with 0.025 M CaC12 in some experiments. The amounts of plasma and these reagents used were twenty or eighty times the amounts indicated in the original directions (Poller and Thomson 1972). After incubation for 15 min at 37°C, the incubation mixture after coagulation was squeezed out of the clot formed with a glass rod.…”

Section: Methodsmentioning

confidence: 99%

Characteristics of phospholipids associated with fibrins in partial thromboplastin test.

Ogawa¹,

Kurokawa²,

Akino

1983

Tohoku J. Exp. Med.

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“…In addition, different activators, incubation times, concentrations of calcium chloride and test dilutions of plasma and phospholipid are recommended for routine laboratory use. The need to standardise both reagents and technique wxs reported in a preliminary survey (Poller & Thomson 1972). Reports by Sibley et al (1973), Babson & Babson (1974), Poller et al (1976), Shapiro et al (1977), Collen et al (1977), O'Brien et al…”

Section: The Need For a Standardmentioning

confidence: 99%

Standardization of the Aptt Test Current Status

Poller

1980

Scandinavian Journal of Haematology

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The partial thromboplastin time (PTT) which is used as an overall measure of the intrinsic clotting system, is the commonest coagulation test employed in routine laboratories apart from the prothrombin time. The main functions of the test are: -1.to screen intrinsic coagulation defects; 2. to control heparin administration; 3. in the control of oral anticoagulant treatment. Many different preparations of phospholipid of human, animal and vegetable origin are used as PTT reagents. In addition, different activators, incubation times, concentrations of calcium chloride and test dilutions of plasma and phospholipid are recommended for routine laboratory use. The need to standardise both reagents and technique has been emphasised in many reports. To meet the need for a reference preparation for the PTT test, a large batch of phospholipid material of human brain origin, designated 71/25, was prepared in the National (UK) Reference Laboratory in lyophilised form in 1971 and proposed as a provisional international reference material. This primary master preparation has been used to calibrate subsequent batches of secondary reference preparations distributed to hospitals in the UK and overseas. Results of a number of collaborative studies have demonstrated the greater reliability of the standardised PTT extract compared with commercial extracts used at the same centres in the three aspects of PTT testing. The conclusion to be drawn from these extensive studies of the laboratory and clinical application of the provisional international reference preparation (71/25) is that we may now be in a position to define an acceptable international standard for the APTT.

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The partial thromboplastin (cephalin) time test

Cited by 27 publications

References 6 publications

An Evaluation of Chromogenic Substrates in the Control of Oral Anticoagulant Therapy

An Evaluation of Chromogenic Substrates in the Control of Oral Anticoagulant Therapy

Characteristics of phospholipids associated with fibrins in partial thromboplastin test.

Standardization of the Aptt Test Current Status

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