The Partition of Tissue Phospholipides by Phosphorus Analysis

The alk-1-enyl group of 1-alk-1(1)-enyl-2-acyl-glycerophospholipids (plasmalogens) rapidly combines with mercuric chloride. At 0 C, there was a 1:1 stoichiometry for Hg binding to the reactive enol group of plasmalogens. Aldehydes were not released, indicating that the alkenyl ether bond was not cleaved. Hg binding to less reactive double bonds in unsaturated fatty acids was not significant. Quantitative estimation of bound Hg afforded a rapid and sensitive assay for alkenylacyl lipids and gave values similar to those obtained with other methods of analysis. The proportion of plasmalogens in bovine myelin glycerophosphatides and in ethanolamine glycerophosphatide was 35 and 75%, respectively. Plasmalogens account for 23.3% of the total glycerophospholipid of rat erythrocytes.

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The formation of cyclic acetals during the acid hydrolysis of lysoplasmalogens

Davenport

Dawson

1962

Biochemical Journal

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The Partition of Tissue Phospholipides by Phosphorus Analysis

Cited by 6 publications

References 27 publications

Revised procedure for the determination of „sphingomyelin” in fresh and formaldehyde-preserved tissue

Revised procedure for the determination of „sphingomyelin” in fresh and formaldehyde-preserved tissue

The quantitative determination of plasmalogen by its reaction with mercuric chloride

The formation of cyclic acetals during the acid hydrolysis of lysoplasmalogens

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