The Passage of Spermatozoa Through the Cervix of Ovariectomized Ewes Treated With Progesterone and Oestrogen

Croker, KP; Robinson, T. J.; Shelton, J. N.

doi:10.1530/jrf.0.0430405

Cited by 10 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recovery from the three regions of the cervix (caudal, middle and cranial) involves a shaking technique which is more efficient than flushing (see Croker, Robinson & Shelton, 1975). The sperm numbers in the flushings and in the stained cervical washings were estimated using the slide techniques described by Mattner & Braden (1963) and Allison (1970 fig.…”

Section: Experiments 1 Controlled Oestrusmentioning

confidence: 99%

“…(1) The authors (Croker, Robinson & Shelton, 1975) Gordon (1971) have shown that the dose of progestagen used to control the time of ovulation and oestrus has an effect on fertility. It follows that, since this lowered fertility is due to faulty sperm transport (Quinlivan & Robinson, 1967, 1969, progestagen insufficiency and faulty sperm transport are related.…”

Section: Experiments 1 Controlled Oestrusmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of Oestrogen Administered During the Progestational Phase of the Cycle on Transport of Spermatozoa in Ewes

Croker¹,

Robinson²,

Shelton³

1975

Reproduction

Self Cite

View full text Add to dashboard Cite

Summary. Two split-plot factorial experiments are described, the first with 72 entire cyclic ewes and the second with 80. The pattern of transport of spermatozoa through the reproductive tract was studied, following treatments with progestagen and oestrogen or with oestrogen alone during 2 weeks preceding insemination. A daily dose of 25 \ g=m\ g oestradiol-17\g=b\ administered to ewes for 14 days preceding oestrus had a deleterious effect on the passage of spermatozoa through the cervix into the uterus within the first 2 hr after insemination. The numbers of spermatozoa recoverable from the cranial region of the cervix 2 hr after insemination appeared to be related to the numbers in the oviducts at 24 hr. These numbers were related to fertility data from an earlier experiment using similar treatments. The data for log numbers of spermatozoa recoverable from the cervix formed a near-normal distribution and so were suitable for formal statistical analysis. There was an interaction between progestagen and oestrogen influence before mating on the pattern of sperm transport through the cervix.

show abstract

Section: Experiments 1 Controlled Oestrusmentioning

confidence: 99%

Section: Experiments 1 Controlled Oestrusmentioning

confidence: 99%

The Effect of Oestrogen Administered During the Progestational Phase of the Cycle on Transport of Spermatozoa in Ewes

Croker¹,

Robinson²,

Shelton³

1975

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…Estrogen is undoubtedly required for efficient sperm transport in the reproductive tract of the estrous ewe, as it is in the ovariectomized ewe (Allison and Robinson, 1972;Croker et al, 1975a). On the other hand, both grazing on pastures high in estrogen content and the administration of 25/ag of estradiol for 14 days before estrus have inhibited sperm transport through the cervix (Lightfoot et al, 1967;Croker et al, 1975b).…”

Section: Discussionmentioning

confidence: 99%

Sperm Death and Inhibition of Sperm Transport in the Cervix of Ewes in Estrus After Removal of the Corpus Luteum-Bearing Ovary

Hawk¹,

Cooper²,

Pursel³

1981

Journal of Animal Science

View full text Add to dashboard Cite

Regulation of estrus in the ewe with prostaglandin or progestogen causes death of sperm in the cervix and inhibits sperm transport through the cervix at the regulated estrus. Because removal of the corpus luteum (CL) alters the estrous cycle, experiments were conducted to determine the effect of removing the CLbearing ovary on sperm survival and transport in the cervix at the ensuing estrus. In the first experiment, the ovary bearing the CL was removed from each of 45 unilaterally ovulating ewes on day 3, 10 or 15 of the estrous cycle (day 0 = first day of estrus). Fifteen ewes from which the non-CL-bearing ovary was removed on day 15 served as controls. Time from surgery until estrus averaged 2.1 to 2.5 days per group. At estrus, each ewe was mated to two rams and necropsied 2 hr later. Sperm were washed from the oviducts, uterus and anterior, middle and posterior one-thirds of the cervix and counted. Removal of the CL-bearing ovary (CLX) decreased (P<.01) the number of sperm recovered, particularly in the anterior one-third of the cervix, where sperm numbers averaged 1.55 million in control ewes, 40,000 in ewes CLX on day 10 and 330,000 and 220,000 in ewes CLX on day 3 or 15. In the next experiment, the CL-bearing ovary was removed from each of 18 ewes on day 10 or 14 of the cycle. Ten ewes from which the non-CLbearing ovary was removed on day 15 served as controls. Time from surgery to estrus averaged 2.0 to 2.3 days per group. At estrus, ewes a Reproduction Lab., Animal Science Institute, AR, SEA, Beltsville Agricultural Research Center. 2 The authors express appreciation to Calrd E. Rexroad, Jr. for statistical analysis of the data; to Douglas Bolt and Rosemary Rollins for conducting the progesterone assays and to Leah Schulman, for evaluating acrosomal morphology. were mated to two rams and necropsied 2 hr later. Sperm were flushed from the uterine body, anterior one-third of the vagina, anterior, middle and posterior one-thirds of the cervix. Condition of sperm was assessed by motility estimates, live-dead staining and normality of apical ridges. CLX on day 10 or 14 significantly reduced the percentages of motile sperm, unstained (live) sperm and sperm with normal apical ridges (NAR); reductions were greater in the uterine body and the anterior one-third of the cervix than in the middle and posterior thirds of the cervix (P<.02, .0001 and .0003, respectively, for percentages of motile, unstained and NAR sperm). In the anterior third of the cervix, percentages of motile sperm for control and CLX ewes averaged 30 and 6, respectively; percentages of unstained sperm averaged 58 and 18, and percentages of sperm with normal apical ridges averaged 44 and 12. Immobilization and death of sperm in the reproductive tract were clearly associated with failure of sperm transport after CLX during the estrous cycle.

show abstract

“…There are a few reports on the use of E 2 in in vitro experiments with sheep. This is because the majority of studies concerning the effect of E 2 in sheep focused on the transfer of sperm in the female reproductive tract (Croker et al, 1975;Hawk & Cooper, 1975;Hawk et al, 1978). In bulls the majority of in vitro studies focused …”

Section: Introductionmentioning

confidence: 99%

The effect of oestradiol-17β on the motility, viability and the acrosomal status of bull sperm

Çiftci

Zülkadir

2010

SA J. An. Sci.

View full text Add to dashboard Cite

The aim of this study was to test the effect of oestradiol-17β (E 2 ) on the motility, viability and the acrosomal status of bull sperm in vitro. Pooled semen from Holstein bulls were incubated in the presence of 2, 4, and 8 μg E 2 /mL for 24 h. Semen was also incubated in media without E 2 . During the incubation, the number of motile, viable sperm and the number of sperm possessing lost/damaged acrosomes, cytoplasmic droplets and coiled tails were counted at 0, 4, 18 and 24 h of incubation. Addition of 2 μg E 2 /mL at 18 h of incubation increased the total motility over the control. The number of forward progressing sperm was increased by the supplementation of 2 and 8 μg E 2 /mL over the control group at the 4 h incubation. Lower doses of E 2 (2 and 4 μg/mL) did not affect viability of sperm, but a high dose of E 2 (8 μg/mL) caused reductions in viability at 4 and 24 h of incubation. The number of sperm cells with lost acrosomes was significantly high in control group at 24 h of incubation. The number of sperm cells possessing proximal and distal cytoplasmic droplets and the number of sperm cells bearing coiled tails were not altered by any of the treatments. A small dose of E 2 (2 μg/mL) had a beneficial effect on the motility and acrosome integrity of bull sperm in vitro. Higher dose of E 2 (8 μg/mL) had a detrimental effect on viability.

show abstract

The Passage of Spermatozoa Through the Cervix of Ovariectomized Ewes Treated With Progesterone and Oestrogen

Cited by 10 publications

References 9 publications

The Effect of Oestrogen Administered During the Progestational Phase of the Cycle on Transport of Spermatozoa in Ewes

The Effect of Oestrogen Administered During the Progestational Phase of the Cycle on Transport of Spermatozoa in Ewes

Sperm Death and Inhibition of Sperm Transport in the Cervix of Ewes in Estrus After Removal of the Corpus Luteum-Bearing Ovary

The effect of oestradiol-17β on the motility, viability and the acrosomal status of bull sperm

Contact Info

Product

Resources

About