The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Trichomonas vaginalisis a kind of flagellate parasite endemic all of the world. study compared the difference of genetic diversity and phylogenetic structure of 18S ribosomal RNA gene ofT. vaginalisfrom Hainan and Xinjiang Provinces, China. 20 samples and 47 samples from Hainan and Xinjiang respectively which confirmed for infection with T.vaginalis. The sequences were aligned using MEGA 11 software and phylogenetic trees were drawn by Neighbor-Joining method. The number of mutation, nucleotide diversity, and haploid diversity were analyzed using Dnaspv5 software. For Hainan samples, the analyses showed 31 polymorphisms, creat different haplotypes with a haplotype diversity of 0.589. Nucleotide diversity and average nucleotide different amongT.vaginaliswere estimated as 0.00679 and 3.447, respectively.Tajima’s D value in Tajiama’s neutrality test was -2.48147,which was significant (p<0.001). But for Xinjiang samples, the analyses showed 31 polymorphisms, creat different haplotypes with a haplotype diversity of 0.731. Nucleotide diversity and average nucleotide different among T.vaginalis were estimated as 0.00497 and 2.50435, respectively. Tajima’s D value in Tajiama’s neutrality test was -2.26284, which was significant (p<0.01). For all the sequence in this study, all the sequences form one phylogenetic tree, some of the sequence from two provinces showed a certain aggregating phenomenon. The differences between the samples from two province maybe for the local environment, ethnic, sample number and so on, it need further study for T.vaginalis.

show abstract

Growth Analysis ofTrichomonas vaginalisin Different Culture Media: Leveraging Large Language Models (LLMs) to Predict and OptimizeIn VitroGrowth Conditions

Ferguson,

Gordon

2024

Preprint

View full text Add to dashboard Cite

Trichomonas vagnalisis a tiny protozoan universally known to have one of the highest prevalance rates of any common sexually transmitted disease. Its popularity in HIV transmission and preterm labor highlights its importance in clinical, biological, and epidemiological investigations worldwide. Propagation of Tvaginalisin vitro uses modified Diamonds media in commercial and clinical culture environments. Several modifications of this medium exist, but a more recent modification proved the most efficient. Our study aimed to investigate media modifications that could optimize the growth ofT. vaginalisin routine laboratory cultivation. RSMT media enriched with quantitated media components was compared to modified Diamond’s, Oxoid, and In Pouch culture mediums. Several growth studies were employed to select eight isolates (n=8) ofT. vaginalis, including an ATCC reference isolate. These isolates were examined for several days at 37 degrees C in an anaerobic environment. Tests revealed that isolates in RSMT media had over 85% higher concentrations of Tvaginalisgrowth than our testing mediums, with a maximum growth increase of 250%. The composite counts ofT. vaginalisin RSMT showed a significant difference (p <0.05) from those ofT. vaginalisin the standard Diamonds media Oxoid or In Pouch mediums. This data suggests that more efficient culturing and growth ofT. vaginalisrequires more vitamins and other growth factors beyond what would conventionally be expended in standard Trichomonas culture mediums.Author’s SummaryIn this study, we explored the growth conditions ofTrichomonas vaginalis, a common sexually transmitted parasite, to find ways to improve its cultivation in the laboratory. By comparing a new growth medium we developed, called RSMT, with existing methods, we identified the best conditions for sustaining this parasite’s growth. Our results showed that RSMT, which includes higher concentrations of essential nutrients, significantly enhanced the growth ofTrichomonas vaginaliscompared to standard media. This advancement is crucial for developing more reliable lab cultures, essential for diagnosing infections and researching new treatments. Additionally, our work demonstrates how innovation in lab practices can optimize clinical procedures and potentially influence public health strategies by improving the management and treatment of infections caused by this parasite.

show abstract

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Cited by 4 publications

References 139 publications

Impact of postsurgical vaginal microbiome on high-risk HPV infection and recurrence risk in patients with cervical cancer and intraepithelial neoplasia: A retrospective study

Impact of postsurgical vaginal microbiome on high-risk HPV infection and recurrence risk in patients with cervical cancer and intraepithelial neoplasia: A retrospective study

comparison of genetic diversity and phylogenetic structure of 18S gene ofTrichomonas vaginalisin Hainan and Xinjiang provinces

Growth Analysis ofTrichomonas vaginalisin Different Culture Media: Leveraging Large Language Models (LLMs) to Predict and OptimizeIn VitroGrowth Conditions

Contact Info

Product

Resources

About