The pathogenesis of turkey origin reoviruses in turkeys and chickens

Spackman, Erica; Pantin‐Jackwood, Mary J.; Day, J. Michael; Sellers, Holly S.

doi:10.1080/03079450500178501

Cited by 43 publications

(37 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…15,39 These TRVs caused significant body weight decreases and moderate to severe bursal atrophy in specific pathogen free (SPF) poults and commercial poults. 44 In this study, we complement these findings by comparing the microscopic lesions induced by these TRVs in SPF poults and determining the viruses spread and replication and its relationship with the lesions observed. We accomplish this by studying the distribution of viral antigen using immunohistochemistry (IHC), viral RNA using in situ hybridization (ISH), and viral-induced apoptosis using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method.…”

mentioning

confidence: 77%

“…Infection status was confirmed by testing all intestinal tissues for virus by real-time reverse-transcriptase polymer chain reaction (RT-PCR). 44 …”

Section: Samplesmentioning

confidence: 99%

“…44 Negative controls consisted of tissues collected from sham-inoculated poults, for which infection status was confirmed to be negative by RT-PCR. The intensity of staining in each section was scored as follows: 2, no antigen staining; +/2, rare; +, infrequent; ++, common; +++, widespread staining.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Tissue samples were collected from SPF Small White Beltsville turkeys inoculated at 2 days of age with 1 of 4 different strains of TRV as described by Spackman et al 44 Briefly, 4 TRV isolates were evaluated: NC/85, TX/ 98, 39 50 ]/bird) by the intratracheal and oral routes (0.1 ml each route). Sham inoculated control birds were inoculated with sterile 50% DMEM/50% F12 cell culture medium.…”

Section: Samplesmentioning

confidence: 99%

“…5 Reovirus has been isolated from turkeys with PEMS 9,42 and its milder form, poult enteritis complex. 15,44 Recently, several TRVs collected from commercial turkey flocks affected with poult enteritis were shown to be genetically distinct from previously reported chicken reoviruses. 15,39 These TRVs caused significant body weight decreases and moderate to severe bursal atrophy in specific pathogen free (SPF) poults and commercial poults.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Pathology and Virus Tissue Distribution of Turkey Origin Reoviruses in Experimentally Infected Turkey Poults

2007

View full text Add to dashboard Cite

Abstract. The pathogenesis of 4 isolates of turkey-origin reovirus (NC/SEP-R44/03, NC/98, TX/98, and NC/85) and 1 chicken-origin reovirus (1733) was examined by infecting specific pathogen free (SPF) poults. These turkey-origin reovirus (TRV) isolates were collected from turkey flocks experiencing poult enteritis and are genetically distinct from previously reported avian reoviruses. Microscopic examination of the tissues collected from the TRV-infected poults revealed different degrees of bursal atrophy characterized by lymphoid depletion and increased fibroplasia between the bursal follicles. To understand the relationship between virus spread and replication, and the induction of lesions, immunohistochemical staining (IHC) for viral antigen, in situ hybridization (ISH) for the detection of viral RNA, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay for the detection of apoptosis in affected tissues was performed. Both IHC and ISH revealed viral antigen and RNA in the surface epithelial cells of the bursa, in macrophages in the interstitium of the bursa and, to lesser degree, in splenic red pulp macrophages and intestinal epithelial cells. Increased apoptosis of bursal lymphocytes and macrophages was observed at 2 and 5 days postinoculation. No lesions were found in tissues from poults inoculated with the virulent chicken-origin strain, however viral antigen was detected in the bursa and the intestine. Although all TRVs studied displayed similar tissue tropism, there were substantial differences in the severity of the lesions produced. Poults inoculated with NC/SEP-R44/03 or NC/98 had moderate to severe bursal atrophy, whereas poults inoculated with TX/ 98 or NC/85 presented a mild to moderate bursal lymphoid depletion. The lymphoid depletion observed in the bursa appears to be the effect of an indirectly induced apoptosis and would most likely result in immune dysfunction in poults infected with TRV.

show abstract

mentioning

confidence: 77%

“…Infection status was confirmed by testing all intestinal tissues for virus by real-time reverse-transcriptase polymer chain reaction (RT-PCR). 44 …”

Section: Samplesmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%