The pathogenic role of different Blastocystis hominis genotypes isolated from patients with irritable bowel syndrome

Fouad, S.S.; Basyoni, Maha M. A.; Fahmy, Reham; Kobaisi, Mohamed H.

doi:10.1016/j.ajg.2011.11.005

Cited by 64 publications

(53 citation statements)

References 32 publications

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“…However, the results are very inconsistent. In Pakistan (Yakoob et al, 2010) and Egypt (Fouad et al, 2011) significant differences in subtype distribution were found, with ST1 being much more common in IBS patients than controls. A study in the UK found ST4 to be more common and ST1 less so in patients from IBS clinics than in other diagnostic laboratory samples, but not reaching statistical significance (Alfellani et al, 2013).…”

Section: Links To Irritable Bowel Syndromementioning

confidence: 92%

Recent Developments in Blastocystis Research

Clark

Giezen

Alfellani

et al. 2013

Advances in Parasitology

251

216

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Abstract:Blastocystis is a common parasite of the human large intestine but has an uncertain role in disease. In this review, we appraise the published evidence addressing this and its weaknesses. Genetic diversity studies have led to the identification of numerous subtypes within the genus Blastocystis and, recently, methods for studying variation within subtypes have been developed, with implications for our understanding of host specificity. The geographic distribution of subtypes is summarised and the impact this may have on investigations into the role of the organism in disease is discussed. Finally, we describe the organelle and nuclear genome characteristics and look to future developments in the field.

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Section: Links To Irritable Bowel Syndromementioning

confidence: 92%

Recent Developments in Blastocystis Research

Clark

Giezen

Alfellani

et al. 2013

Advances in Parasitology

251

216

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“…Regarding ST4, it was detected in only one individual in our Tunisian cohort and still unidentified in Egypt and Libya, thus confirming the virtual absence of ST4 in north African countries. [19][20][21][22] More globally, ST4 is much less frequently detected or absent in Africa, Middle East, Asia, and North America while it is commonly found in Europe. 5,11 The recently proposed ST4 emergence in the European population might explain such heterogeneous geographical distribution of this ST. 2 Finally, ST7 was identified in only one case in our Tunisian cohort.…”

mentioning

confidence: 99%

“…19 On the other hand, ST3 was largely predominant in the three studies conducted in different Egyptian areas. [20][21][22] Since ST3 is rarely identified in animals that may be in contact with humans, a large-scale interhuman transmission likely explains the predominance of this ST worldwide. 1,2,11 As stated above, ST1 represented 29.6% of the subtyped isolates colonizing Tunisian food handlers.…”

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confidence: 99%

“…The ST distribution in Tunisia can be compared with those obtained in the few studies conducted in other Maghreb countries such as Libya 11,19 and in neighboring countries including Egypt. [20][21][22] It is worth noting that those molecular data were obtained after Blastocystis culturing in liquid medium, which could result in a selection bias of some STs. In Libya, ST1 showed the highest frequency (more than 50% of the isolates), followed by either ST3 11 or ST2.…”

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confidence: 99%

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Prevalence and Subtype Identification of Blastocystis sp. in Healthy Individuals in the Tunis Area, Tunisia

Abda¹,

Maatoug²,

Romdhane³

et al. 2017

The American Society of Tropical Medicine and Hygiene

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Abstract. Blastocystis sp. is currently the most common eukaryotic parasite found in humans. Despite its potential public health impact, epidemiological data regarding its prevalence and molecular subtype (ST) distribution in Maghreb are rarely reported. Therefore, the aim of this study was to determine the prevalence of the parasite in a cohort of healthy food handler Tunisian individuals and to acquire the first molecular data regarding the distribution of Blastocystis sp. STs in this country. Therefore, 524 fecal samples were collected, and 68 of them (13%) were identified as positive for the parasite by direct-light microscopy of smears. Seventeen samples of 100 negative by microscopy were also shown to be positive by real-time quantitative polymerase chain reaction. Among all the positive samples, 61 Blastocystis isolates were subtyped using partial small subunit ribosomal RNA gene analysis. ST3 was the most abundant (51%) followed by ST1 (30%), ST2 (16%), and ST4 and ST7 (both 1.6%).Blastocystis sp. is a parasite inhabiting the gastrointestinal tract of humans and a wide range of animals. This singlecelled eukaryote is currently the most prevalent protozoa found in human fecal samples with a worldwide distribution.

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“…Organisms of the subtype 1, 2 or 5 were isolated from pigs. Of these, the subtype 1, which is cross-infective between animals and birds, has been frequently isolated from patients exhibiting symptoms of irritable bowel syndrome (Fouad et al 2011). Subtype 2, which is also the most commonly isolated subtype from monkeys (Yoshikawa et al 2009), has also been associated with human infection.…”

Section: Resultsmentioning

confidence: 99%

Blastocystis sp. from food animals in India

et al. 2013

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Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai.The 'central vacuole forms' of the parasite, with number of nuclei ranging from 1 to 12 were identified. The intensity of infection was low, with less than one organism per oil immersion field, indicating that their presence was unconnected to the cause of death. Caecal scraping was found to be more ideal than duodenal scraping for the diagnosis of Blastocystis, and can be a potential specimen for definitive diagnosis. Identical organisms were also detected in the dung samples of a buffalo calf which showed clinical signs of diarrhoea The presence of Blastocystis in food animals acquires public health significance, as many subtypes of the parasite from poultry and pigs are transmissible to humans.

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The pathogenic role of different Blastocystis hominis genotypes isolated from patients with irritable bowel syndrome

Cited by 64 publications

References 32 publications

Recent Developments in Blastocystis Research

Recent Developments in Blastocystis Research

Prevalence and Subtype Identification of Blastocystis sp. in Healthy Individuals in the Tunis Area, Tunisia

Blastocystis sp. from food animals in India

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