The pathogenic Yersiniae—advances in the understanding of physiology and virulence

Francis, Matthew S.

doi:10.3389/fcimb.2013.00051

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…The enteropathogen Yersinia enterocolitica is a member of the genus Yersinia , which includes two other human pathogens: the plague bacillus Y. pestis and Y . pseudotuberculosis , a gastrointestinal pathogen (Bottone, ; Francis, ). Yersinia enterocolitica is a heterogeneous species classified into 60 serotypes and six biotypes that vary in pathogenicity (Thomson et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Impact of OmpR on the membrane proteome of Yersinia enterocolitica in different environments: repression of major adhesin YadA and heme receptor HemR

Nieckarz

Raczkowska

Dębski

et al. 2016

Environmental Microbiology

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Enteropathogenic Yersinia enterocolitica is able to grow within or outside the mammalian host. Previous transcriptomic studies have indicated that the regulator OmpR plays a role in the expression of hundreds of genes in enterobacteria. Here, we have examined the impact of OmpR on the production of Y. enterocolitica membrane proteins upon changes in temperature, osmolarity and pH. Proteomic analysis indicated that the loss of OmpR affects the production of 120 proteins, a third of which are involved in uptake/transport, including several that participate in iron or heme acquisition. A set of proteins associated with virulence was also affected. The influence of OmpR on the abundance of adhesin YadA and heme receptor HemR was examined in more detail. OmpR was found to repress YadA production and bind to the yadA promoter, suggesting a direct regulatory effect. In contrast, the repression of hemR expression by OmpR appears to be indirect. These findings provide new insights into the role of OmpR in remodelling the cell surface and the adaptation of Y. enterocolitica to different environmental niches, including the host.

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Section: Introductionmentioning

confidence: 99%

Impact of OmpR on the membrane proteome of Yersinia enterocolitica in different environments: repression of major adhesin YadA and heme receptor HemR

Nieckarz

Raczkowska

Dębski

et al. 2016

Environmental Microbiology

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“…The genus Yersinia includes three human pathogenic species: the plague causative agent Y. pestis , along with Y. enterocolitica and Y. pseudotuberculosis , which cause food‐borne gastrointestinal disease (Francis, ). All share a common Ysc‐Yop T3SS virulence mechanism, which is encoded by a conserved ∼70 kb virulence plasmid.…”

Section: Introductionmentioning

confidence: 99%

RcsB positively regulates the Yersinia Ysc‐Yop type III secretion system by activating expression of the master transcriptional regulator LcrF

Francis

et al. 2014

Environmental Microbiology

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The Rcs phosphorelay is a complex signaling pathway used by the family Enterobacteriaceae to sense, respond and adapt to environmental changes during free-living or host-associated lifestyles. In this study, we show that the Rcs phosphorelay pathway positively regulates the virulence plasmid encoded Ysc-Yop type III secretion system (T3SS) in the enteropathogen Yesinia pseudotuberculosis. Both the overexpression of the wild-type Rcs regulator RcsB or the constitutive active RscB(D56E) variant triggered more abundant Ysc-Yop synthesis and secretion, whereas the non-phosphorylatable mutant RcsB(D56Q) negated this. Congruently, enhanced Yops expression and secretion occurred in an in cis rscB(D56E) mutant but not in an isogenic rscB(D56Q) mutant. Screening for regulatory targets of RcsB identified the virG-lcrF operon that encodes for LcrF, the Ysc-Yop T3SS master regulator. Protein-DNA binding assays confirmed that RcsB directly bound to this operon promoter, which subsequently caused stimulated lcrF transcription. Moreover, active RcsB enhanced the ability of bacteria to deliver Yop effectors into immune cells during cell contact, and this promoted an increase in bacterial viability. Taken together, our study demonstrates the role of the Rcs system in regulating the Ysc-Yop T3SS in Yersinia and reports on RcsB being the first transcriptional activator known to directly control lcrF transcription.

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“…For humans, pathogenic Y. pseudotuberculosis has a wide range of pathogenicity factors determined by chromosomal and plasmid genes [ 1 , 47 , 48 , 49 ]. By introducing new data on genetic diversity within the Yersinia pseudotuberculosis complex, authors [ 50 , 51 ] have reported a monomorphic clade that caused FESLF in the USSR in 1959, which often undergoes recombination possibly due to the conjugative plasmid pVM82 and contains the TcpI virulence protein.…”

Section: Plasmid-associated Virulence Of Y Pseudotubercmentioning

confidence: 99%

Far Eastern Scarlet-Like Fever is a Special Clinical and Epidemic Manifestation of Yersinia pseudotuberculosis Infection in Russia

Сомова¹,

Antonenko

Тимченко³

et al. 2020

Pathogens

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Pseudotuberculosis in humans until the 1950s was found in different countries of the world as a rare sporadic disease that occurred in the form of acute appendicitis and mesenteric lymphadenitis. In Russia and Japan, the Yersinia pseudotuberculosis (Y. pseudotuberculosis) infection often causes outbreaks of the disease with serious systemic inflammatory symptoms, and this variant of the disease has been known since 1959 as Far Eastern Scarlet-like Fever (FESLF). Russian researchers have proven that the FESLF pathogen is associated with a concrete clonal line of Y. pseudotuberculosis, characterized by a specific plasmid profile (pVM82, pYV 48 MDa), sequence (2ST) and yadA gene allele (1st allele). This review summarized the most important achievements in the study of FESLF since its discovery in the Far East. It has been established that the FESLF causative agent is characterized by a unique phenomenon of psychrophilicity, which consists of its ability to reproduce in the environment with its biologically low and variable temperature (4–12 °C), at which the pathogen multiplies and accumulates while maintaining or increasing its virulence, which ensures the emergence and development of the epidemic process. The key genetic and biochemical mechanisms of Y. pseudotuberculosis adaptation to changing environmental conditions were characterized, and the morphological manifestations of the adaptive variability of these bacteria in different conditions of their habitat were revealed. The main features of the pathogenesis and morphogenesis of FESLF, including those associated with the Y. pseudotuberculosis toxigenicity, were presented. The pathogenetic value of the plasmid PVM82, found only in the FESLF pathogen, was shown.

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The pathogenic Yersiniae—advances in the understanding of physiology and virulence

Cited by 7 publications

References 22 publications

Impact of OmpR on the membrane proteome of Yersinia enterocolitica in different environments: repression of major adhesin YadA and heme receptor HemR

Impact of OmpR on the membrane proteome of Yersinia enterocolitica in different environments: repression of major adhesin YadA and heme receptor HemR

RcsB positively regulates the Yersinia Ysc‐Yop type III secretion system by activating expression of the master transcriptional regulator LcrF

Far Eastern Scarlet-Like Fever is a Special Clinical and Epidemic Manifestation of Yersinia pseudotuberculosis Infection in Russia

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