The pathophysiology of Fuchs' endothelial dystrophy – A review of molecular and cellular insights

Zhang, Jie; Patel, Dipika V.

doi:10.1016/j.exer.2014.10.023

Cited by 70 publications

(79 citation statements)

References 69 publications

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“…Therefore, the limiting effect of preoperative visual acuity seems to be on corneal dysfunction and no other eye disease. Although there was no significant difference between the grading groups according to the preoperative central corneal thickness and no significant correlation between the grade of difficulty and the preoperative central corneal thickness, there was a tendency towards a larger preoperative central corneal thickness and, therefore, a more edematous cornea in group grade IV compared to group I. Additionally, it should be taken into account that not only the edema has an influence on the degree of pre-operative corneal dysfunction and, therefore, the preoperative visual acuity in patients with Fuchs endothelial dystrophy and bullous keratopathy, but also the grade of ultrastructural matrix changes, such as formation of epithelial bullae, sub-epithelial fibroblast and collagen infiltration, reduction of sub-basal corneal nerve density, reduced anterior keratocyte density and fibroblastic transformation of stressed keratocytes in the stroma [14]. As reported, there was no significant correlation between difficulty of unfolding and attachment of the graft lamella and the indication for DMEK.…”

Section: Discussionmentioning

confidence: 99%

Influence of the difficulty of graft unfolding and attachment on the outcome in descemet membrane endothelial keratoplasty

Maier

Gundlach

Schroeter

et al. 2015

Graefes Arch Clin Exp Ophthalmol

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There is no correlation between corneal donor tissue characteristics and the degree of difficulty of unfolding using graft lamella older than 49 years. Therefore, it is not possible to select grafts best suited for DMEK surgery on the basis of donor characteristics when the donor age is above 50 years. Preoperative visual acuity influences the grade of difficulty. The rate of graft detachments and endothelial cell loss increases significantly with a more difficult graft unfolding. The proposed grading system may therefore be relevant for postoperative management.

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Section: Discussionmentioning

confidence: 99%

Influence of the difficulty of graft unfolding and attachment on the outcome in descemet membrane endothelial keratoplasty

Maier

Gundlach

Schroeter

et al. 2015

Graefes Arch Clin Exp Ophthalmol

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“…The cause of the disease is believed to involve hereditary and hormonal and environmental factors. 4 The corneal endothelium is continuously exposed to endogenous and exogenous oxidative stress. Its deturgescence activity induces a high aerobic metabolism.…”

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confidence: 99%

Restoration of Mitochondrial Integrity, Telomere Length, and Sensitivity to Oxidation by In Vitro Culture of Fuchs' Endothelial Corneal Dystrophy Cells

Gendron

Thériault

Proulx

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Citation: Gendron SP, Thériault M, Proulx S, Brunette I, Rochette PJ. Restoration of mitochondrial integrity, telomere length, and sensitivity to oxidation by in vitro culture of Fuchs' endothelial corneal dystrophy cells. Invest Ophthalmol Vis Sci. 2016;57:5926-5934. DOI:10.1167/ iovs.16-20551 PURPOSE. Fuchs' endothelial corneal dystrophy (FECD), a degenerative disease of the corneal endothelium that leads to vision loss, is a leading cause of corneal transplantation. The cause of this disease is still unknown, but the implication of oxidative stress is strongly suggested. In this study, we analyzed the impact of FECD on mitochondrial DNA (mtDNA) integrity and telomere length, both of which are affected by the oxidative status of the cell. METHODS.We compared the levels of total mtDNA, mtDNA common deletion (4977 bp), and relative telomere length in the corneal endothelial cells of fresh Descemet's membraneendothelium explants and cultured cells from healthy and late stage FECD subjects. Oxidantantioxidant gene expression and sensitivity to ultraviolet A (UVA)-and H 2 O 2 -induced cell death were assessed in cultured cells. RESULTS.Our results revealed increased mtDNA levels and telomere shortening in FECD explants. We also found that cell culture restores a normal phenotype in terms of mtDNA levels, telomere length, oxidant-antioxidant gene expression balance, and sensitivity to oxidative stress-induced cell death in the FECD cells compared with the healthy cells.CONCLUSIONS. Taken together, these results bring new evidence of the implication of oxidative stress in FECD. They also show that FECD does not evenly affect the integrity of corneal endothelial cells and that cell culture can rehabilitate the molecular phenotypes related to oxidative stress by selecting the more functional FECD cells.

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“…This finding is in agreement with the clinical finding of DM thickening in Fuchs endothelial corneal dystrophy. 17,18 Another observation was that the thickness of the thick membrane also considerably increased after exposure to the nutritional fluid, possibly further impeding dehydration of the stroma by the endothelial cells. As was expected, there was complete disorganization of the stromal layer in the absence of endothelial cells.…”

Section: Discussionmentioning

confidence: 97%

In Vitro Study of the Deturgescence Ability of Cultivated Human Corneal Endothelial Cells

Tsaousis

Kopsachilis

Tsinopoulos

et al. 2016

Cornea

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The pathophysiology of Fuchs' endothelial dystrophy – A review of molecular and cellular insights

Cited by 70 publications

References 69 publications

Influence of the difficulty of graft unfolding and attachment on the outcome in descemet membrane endothelial keratoplasty

Influence of the difficulty of graft unfolding and attachment on the outcome in descemet membrane endothelial keratoplasty

Restoration of Mitochondrial Integrity, Telomere Length, and Sensitivity to Oxidation by In Vitro Culture of Fuchs' Endothelial Corneal Dystrophy Cells

In Vitro Study of the Deturgescence Ability of Cultivated Human Corneal Endothelial Cells

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